Results 241 to 250 of about 4,243,841 (306)

Mutagenic and physiological impacts of colchicine on growth, flowering behavior, and ISSR-based genetic variability in Calendula officinalis L. [PDF]

BMC Plant Biol
Hassan MA, Saudy HS, Hewidy M, El-Sammak H.   +3 more
europepmc   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

A new highly discriminatory typing scheme for <i>Treponema pallidum</i> reveals similar levels of genetic variability across lineages. [PDF]

mBio
Pla-Díaz M, Giacani L, Tantalo LC, Bose M, Reid TB, Marra CM, Šmajs D, Pospíšilová P, Janečková K, Kawahata T, Banno F, Vilfort K, Cao W, Pillay A, Noda A, Bosshard PP, Chen M, Mitjà O, Schuenemann VJ, Hackl S, Nieselt K, Hernández-Bel P, Ocete MD, Arora N, González-Candelas F.   +24 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

APOE genetic variability in an Egyptian cohort of PD. [PDF]

Front Neurosci
Khedr EM, William MB, Elhosseiny AA, Shalash A, Fawi G, Yousef MH, El-Jaafary S, Lee H, Jama A, Koraym M, Helmy A, Salah Y, George P, Haridy NA, Nabhan S, Atputhavadivel A, Elfarrash S, Ragab G, Hegazy MT, Elsaid Y, Gabr AS, Shebl N, Aly L, Abdelwahhab N, Belal TM, Elsayed NAB, El-Gamal M, Elgamal S, Ragab S, Mekky J, Houlden H, Rizig M, Salama M.   +32 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Tissue-Specific Anthocyanin and Polyphenol Content in Sweet Cherry (<i>Prunus avium</i> L.): Effects of Freezing and Association with MYB-Based Genetic Variability. [PDF]

Molecules
Mihályfi C, Bedő J, Halász GE, Daood HG, Mendel Á, Tarekegn MM, Tóth-Lencsés AK, Kovács Z, Neményi A, Veres A.   +9 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Associations Between OPN-CD44 Axis Genetic Variability, Plasma Osteopontin, and Treatment Outcomes in Head and Neck Squamous Cell Carcinoma. [PDF]

Int J Mol Sci
Gdowicz-Kłosok A, Deja R, Rutkowski T, Bugowska M, Mrochem-Kwarciak J, Składowski K, Butkiewicz D.   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source