Results 151 to 160 of about 4,784,986 (369)
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
pMAGs: A Versatile and Efficient Vector System for Multi-Gene Studies in Plants
In molecular biology studies, suitable vectors are fundamental tools; however, most vectors can only express one target gene, which limits the ability to study multiple genes simultaneously within the same plant tissue.
Mengyue Zhang +5 more
doaj +1 more source
Potent Vaccine Therapy with Dendritic Cells Genetically Modified by the Gene-Silencing-Resistant Retroviral Vector GCDNsap [PDF]
Tsukasa Nabekura +4 more
openalex +1 more source
Population genetic structure of the malaria vector Anopheles minimus in Thailand based on mitochondrial DNA markers [PDF]
Kamonchanok Bunmee +7 more
openalex +1 more source
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama +7 more
wiley +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
High rate of genetic rearrangement during replication of a Moloney murine leukemia virus-based vector [PDF]
Alfredo Varela‐Echavarría +3 more
openalex +1 more source

