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Poxviruses as Genetic Vectors

1995
Since the pioneering studies of Edward Jenner on using live virus vaccines during the early 1800s (Baxby 1981), a considerable amount of information has been obtained on the biology and replication of poxviruses. Vaccinia virus, the agent used to vaccinate humans against smallpox and eradicate this disease, has been widely studied in the laboratory due
William I. Cox   +2 more
openaire   +3 more sources

Genetic transformation of Drosophila with transposable element vectors.

Science, 1982
Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 ...
G. Rubin, A. Spradling
semanticscholar   +1 more source

Genetic technologies for disease vectors

Current Opinion in Insect Science, 2015
The first genetic technologies for insect vectors of disease were introduced 20 years ago. As of today there are 12 classes of genetic technologies used as functional genomic tools for insect vectors of important diseases. Although the applications of genetic technologies in insect disease vectors have been conducted primarily in mosquitoes, other ...
William Reid   +2 more
openaire   +3 more sources

Exosome-mediated delivery of gene vectors for gene therapy.

Nanoscale, 2020
Gene vectors are nucleic acids that carry genetic materials or gene editing devices into cells to exert the sustained production of therapeutic proteins or to correct erroneous genes of the cells.
Li Duan   +7 more
semanticscholar   +1 more source

Genetic and Physical Map of the pLAFR1 Vector

DNA Sequence, 2004
This paper presents the complete sequencing and annotation of the pLAFR1 vector. pLAFR is a tetracycline-resistant "cosmid" cloning vector, which is derived from the 20 kb plasmid pRK290, a RK2-derivative. Due to its broad host range, the pLAFR1 vector has been widely used in the genetic analysis of a broad number of gram-negative bacterial species ...
Jozef Vanderleyden   +2 more
openaire   +3 more sources

Viral Vectors for Gene Transfer

Current Protocols in Mouse Biology, 2018
Viral vectors are a promising tool for effective delivery of genetic material into cells. They take advantage of the natural ability of a virus to deliver a genetic payload into cells while being genetically modified such that their ability to replicate ...
Yong Hong Chen   +2 more
semanticscholar   +1 more source

A review of food-grade vectors in lactic acid bacteria: from the laboratory to their application.

Critical Reviews in Biotechnology, 2017
Lactic acid bacteria (LAB) have a long history of use in fermented foods and as probiotics. Genetic manipulation of these microorganisms has great potential for new applications in food safety, as well as in the development of improved food products and ...
J. M. Landete
semanticscholar   +1 more source

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.

ACS Synthetic Biology, 2017
Mammalian plasmid expression vectors are critical reagents underpinning many facets of research across biology, biomedical research, and the biotechnology industry.
A. Martella   +4 more
semanticscholar   +1 more source

Vector Genetics in Malaria Control

2006
Anopheles culicifacies is the major vector of rural malaria in the plains of India. Cytogenetic analysis of polytene chromosomes revealed that A. culicifacies is a complex of five sibling species designated as A, B, C, D, and E. Each sibling species has its own distribution pattern which is influenced by the environment. Sibling species differ in their
openaire   +2 more sources

Genetic Modification of Chondrocytes Using Viral Vectors

2010
The use of isolated cells to construct engineered tissues provides the opportunity to genetically modify those cells prior to the formation of tissue. This should make it possible to create transgenic human model tissues that can be used to determine gene function as well as to identify or validate potential therapeutic targets.
Aileen Crawford   +3 more
openaire   +3 more sources

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