Results 11 to 20 of about 143,498 (285)

Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses

The Scientific World Journal, 2014
Background. Ex vivo culture of intact embryonic kidney has become a powerful system for studying renal development. However, few methods have been available for gene manipulation and have impeded the identification and investigation of genes in this ...
Tie-Lin Chen, Hong-Lian Wang, Yun-Hong Liu, Yin Fang, Rui-Zhi Tan, Pu-Hui Zhou, Qin Zhou, Xiao-Yan Lv   +7 more
doaj   +1 more source

Genetically Engineered Plasmonic Nanoarrays [PDF]

Nano Letters, 2012
In the present Letter, we demonstrate how the design of metallic nanoparticle arrays with large electric field enhancement can be performed using the basic paradigm of engineering, namely the optimization of a well-defined objective function. Such optimization is carried out by coupling a genetic algorithm with the analytical multiparticle Mie theory ...
Carlo Forestiere, Alyssa J. Pasquale, Antonio Capretti, Giovanni Miano, TAMBURRINO, Antonello, Sylvanus Y. Lee, Björn M. Reinhard, Luca Dal Negro   +7 more
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Development and Adoption of Genetically Engineered Plants for Virus Resistance: Advances, Opportunities and Challenges

Plants, 2021
Plant viruses cause yield losses to crops of agronomic and economic significance and are a challenge to the achievement of global food security. Although conventional plant breeding has played an important role in managing plant viral diseases, it will ...
Prakash M. Niraula, Vincent N. Fondong
doaj   +1 more source

Encapsulated and ‘free’ pig islet xenotransplantation: recent experience and clinical progress* [PDF]

CellR4, 2019
Genetically-engineered pigs offer a possible alternative to deceased human donors as a source of isolated islets for transplantation into patients with life-threatening diabetes.
D. Cooper, R. Bottino
doaj   +1 more source

First Year Course-Based Undergraduate Research Experience (CURE) Using the CRISPR/Cas9 Genome Engineering Technology in Zebrafish

Journal of Microbiology & Biology Education, 2018
Genetic analysis in model systems can provide a rich context for conceptual understanding of gene structure, regulation, and function. With an intent to create a rich learning experience in molecular genetics, we developed a semester-long course-based ...
Jay M. Bhatt, Anil Kumar Challa
doaj   +1 more source

Engineering Switches, Genetically [PDF]

Chemistry & Biology, 2004
Ostermeier, Guntas, and Mitchel describe a new approach to design enzymes that are allosterically regulated by an unrelated ligand . The resulting protein, constructed by nonhomologous recombination and genetic screens, displays switch-like behavior.
openaire   +2 more sources

CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity

Nature Communications, 2019
Genetically engineered T cells expressing a chimeric antigen receptor (CAR-T cells) are a promising new treatment for cancer, but are associated with unique toxicities. Here, the authors test CAR-T-cell-derived exosomes as a surrogate for CAR-T cells and
Wenyan Fu, Changhai Lei, Shuowu Liu, Yingshu Cui, Chuqi Wang, Kewen Qian, Tian Li, Yafeng Shen, Xiaoyan Fan, Fangxing Lin, Min Ding, Mingzhu Pan, Xuting Ye, Yongji Yang, Shi Hu   +14 more
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Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells

iScience
Summary: The insulin receptor (INSR, IR) has two isoforms, IRA and IRB, through alternative splicing. However, their distinct functions in vivo remain unclear. Here we generated β cell-specific IRB knockout (KO) mice (βIRBKO).
Mingchao Jiang, Ning Wang, Yuqin Zhang, Jinjin Zhang, Youwei Li, Xiu Yan, Honghao Zhang, Chengbin Li, Youfei Guan, Bin Liang, Weiping Zhang, Yingjie Wu   +11 more
doaj   +1 more source

Identification of diosgenin isolated from genetically engineered tissues cultures of Trigonella foenum-graecum L.

مجلة مركز بحوث التقنيات الاحيائية, 2014
Thediosgenin compound was separated, one of the secondary metabolic product in Trigonella foenum-graecum L. plants. Hairy roots cultures transformed by Agrobacterium rhizogenes R1601, and their spontaneously formed callus contained "diosgenin".
M. M. Al-Mahdawe, M. K. Al-Mallah, A. O. Al-Attrakchii   +2 more
doaj   +1 more source

Forecasting SARS-CoV-2 spike protein evolution from small data by deep learning and regression

Frontiers in Systems Biology
The emergence of SARS-CoV-2 variants during the COVID-19 pandemic caused frequent global outbreaks that confounded public health efforts across many jurisdictions, highlighting the need for better understanding and prediction of viral evolution ...
Samuel King, Samuel King, Samuel King, Xinyi E. Chen, Xinyi E. Chen, Xinyi E. Chen, Sarah W. S. Ng, Sarah W. S. Ng, Sarah W. S. Ng, Kimia Rostin, Kimia Rostin, Kimia Rostin, Samuel V. Hahn, Samuel V. Hahn, Tylo Roberts, Tylo Roberts, Janella C. Schwab, Janella C. Schwab, Parneet Sekhon, Parneet Sekhon, Madina Kagieva, Madina Kagieva, Madina Kagieva, Taylor Reilly, Taylor Reilly, Taylor Reilly, Ruo Chen Qi, Ruo Chen Qi, Paarsa Salman, Paarsa Salman, Paarsa Salman, Ryan J. Hong, Ryan J. Hong, Eric J. Ma, Steven J. Hallam, Steven J. Hallam, Steven J. Hallam, Steven J. Hallam, Steven J. Hallam, Steven J. Hallam   +39 more
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