Results 91 to 100 of about 279,987 (320)

Inhibition of in vitro fertilizing capacity of cryopreserved mouse sperm by factors released by damaged sperm, and stimulation by glutathione. [PDF]

PLoS ONE, 2010
BACKGROUND: In vitro fertilization (IVF) of eggs by frozen and thawed C57BL/6J mouse sperm is inhibited by dead sperm and enhanced by preincubation of the sperm in calcium-free medium.
Mary L Bath
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse

MethodsX, 2019
Genetically modified model organisms are valuable tools for probing gene function, dissecting complex signaling networks, studying human disease, and more.
Kyriel M. Pineault, Ana Novoa, Anastasiia Lozovska, Deneen M. Wellik, Moises Mallo   +4 more
doaj   +1 more source

Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously Infected with HIV-1. [PDF]

, 2018
Investigations of anti-HIV-1 human hematopoietic stem/progenitor cell (HSPC)-based gene therapy have been performed by HIV-1 challenge after the engraftment of gene-modified HSPCs in humanized mouse models.
An, Dong Sung, Arokium, Hubert, Chen, Irvin SY, Cortado, Ruth, Jung, Yujin, Kamata, Masakazu, Khamaikawin, Wannisa, Kim, Patrick, Kim, Sanggu, Lam, Jennifer, Presson, Angela P, Shimizu, Saki, Wen, Jing, Xie, Yiming   +13 more
core   +2 more sources

Genetically modified mouse models in studies of luteinising hormone action

Molecular and Cellular Endocrinology, 2006
Numerous genetically modified mouse models have recently been developed for the study of the pituitary-gonadal interactions. They include spontaneous or engineered knockouts (KO) of the gonadotrophin-releasing hormone (GnRH) and its receptor, the gonadotrophin common-alpha(Calpha), luteinising hormone (LH) beta and follicle-stimulating hormone (FSH ...
Huhtaniemi, Ilpo, Ahtiainen, Petteri, Pakarainen, Tomi, Rulli, Susana Beatriz, Zhang, Fu Ping, Poutanen, Mattu   +5 more
openaire   +3 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Specific transfection of inflamed brain by macrophages: a new therapeutic strategy for neurodegenerative diseases. [PDF]

PLoS ONE, 2013
The ability to precisely upregulate genes in inflamed brain holds great therapeutic promise. Here we report a novel class of vectors, genetically modified macrophages that carry reporter and therapeutic genes to neural cells.
Matthew J Haney, Yuling Zhao, Emily B Harrison, Vivek Mahajan, Shaheen Ahmed, Zhijian He, Poornima Suresh, Shawn D Hingtgen, Natalia L Klyachko, R Lee Mosley, Howard E Gendelman, Alexander V Kabanov, Elena V Batrakova   +12 more
doaj   +1 more source

Approaches for timeline reductions in pathogenesis studies using genetically modified mice

Microbiology Spectrum, 2023
Although genetically modified mouse models have long been a powerful tool for microbiology research, the manipulation of the mouse genome is expensive, time consuming, and has historically remained the domain of dedicated animal facilities.
Samantha Skavicus, Nicholas S. Heaton
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Simultaneous Detection of Multiple Transgenes for Genetically-Modified Mouse Strains

Experimental Animals, 2009
Recent advances in the genetic manipulation of mice have enabled us to generate transgenic and knockout mice. However, it is not easy to maintain these genetically-modified mice with the high-quality necessary to meet both scientific and legal requirements. RIKEN BRC has collected various transgenic, knockout, and conditional knockout mice.
Hatsumi, Nakata, Tomomi, Hashimoto, Yukiko, Seki, Kazuyuki, Mekada, Yuichi, Obata, Atsushi, Yoshiki   +5 more
openaire   +3 more sources