Results 101 to 110 of about 1,279,027 (330)

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Nature Communications, 2018
Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.
Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels   +14 more
doaj   +1 more source

Inflammation-associated enterotypes, host genotype, cage and inter-individual effects drive gut microbiota variation in common laboratory mice [PDF]

, 2013
Background: Murine models are a crucial component of gut microbiome research. Unfortunately, a multitude of genetic backgrounds and experimental setups, together with inter-individual variation, complicates cross-study comparisons and a global ...
Brinkman, Brigitta, Cauwe, Benedicte, Garcia Yunta, Roberto, Hildebrand, Falk, Liston, Adrian, Nguyen, (Thi Loan Anh, Raes, Jeroen, Vandenabeele, Peter   +7 more
core   +3 more sources

Effect of host genetics on the gut microbiome in 7,738 participants of the Dutch Microbiome Project

bioRxiv, 2020
Host genetics are known to influence the gut microbiome, yet their role remains poorly understood. To robustly characterize these effects, we performed a genome-wide association study of 207 taxa and 205 pathways representing microbial composition and ...
Lopera Maya, Kurilshikov, van der Ferdinand Graaf, Hu, Andreu-Sanchez, Chen, Vich Villa, Gacesa, Sinha, Collij, Klassen, Bolte, Brandao Gois, Neerincx, Swertz, Harmsen, Wijmenga, Fu, Weersma, Zhernakova, Sanna   +20 more
semanticscholar   +1 more source

Clinical Practice Guideline for Evaluation and Management of Peripheral Nervous System Manifestations in Sjögren's Disease

Arthritis Care &Research, Accepted Article.
Objectives Sjögren's disease is an autoimmune disorder that can impact multiple organ systems, including the peripheral nervous system (PNS). PNS manifestations, which can exist concurrently, include mononeuropathies, polyneuropathies, and autonomic nervous system neuropathies. To help patients and providers in the decision‐making process, we developed
Anahita Deboo, Robert Fox, Katherine M. Hammitt, Julie Frantsve‐Hawley, Matthew C. Baker, Stamatina Danielides, Eduardo De Sousa, Brent P. Goodman, Jennifer K. King, Steven Mandel, Ghaith Noaiseh, Pantelis P. Pavlakis, George Sarka, R. Hal Scofield, Arun Varadhachary, Daniel J. Wallace, Matt Makara, Nancy Carteron, Steven Carsons, In collaboration with the Consensus Expert Panel (CEP) members   +19 more
wiley   +1 more source

Clinical metagenomics. [PDF]

, 2019
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing
Chiu, Charles Y, Miller, Steven A
core  

The biotechnology of higher fungi - current state and perspectives [PDF]

, 2014
This review article concisely describes methodology of biotechnological processes with the use of cultures of higher fungi, their application in bioremediation and to obtain biologically active preparations.
Turło, Jadwiga
core   +1 more source

Diet, Gut Microbiota, and Obesity: Links with Host Genetics and Epigenetics and Potential Applications.

Advances in Nutrition, 2019
Diverse evidence suggests that the gut microbiota is involved in the development of obesity and associated comorbidities. It has been reported that the composition of the gut microbiota differs in obese and lean subjects, suggesting that microbiota ...
A. Cuevas-Sierra, O. Ramos-López, J. Riezu-Boj, F. Milagro, F. Milagro, J. Martínez   +5 more
semanticscholar   +1 more source

Advancing Research on Biomaterials and Biological Materials with Scanning Electron Microscopy under Environmental and Low Vacuum Conditions

Advanced Engineering Materials, EarlyView.
Herein, environmental scanning electron microscopy (ESEM) is discussed as a powerful extension of conventional SEM for life sciences. By combining high‐resolution imaging with variable pressure and humidity, ESEM allows the analysis of untreated biological materials, supports in situ monitoring of hydration‐driven changes, and advances the functional ...
Jendrian Riedel, Lea Dierker, Antoine Eyram Kwame, Kim Nora Wagner, Jonas J. Unterholzner, Jan‐Henning Dirks, Dorothea Brüggemann   +6 more
wiley   +1 more source

Microbial Nitrogen Cycling in Antarctic Soils

Microorganisms, 2020
The Antarctic continent is widely considered to be one of the most hostile biological habitats on Earth. Despite extreme environmental conditions, the ice-free areas of the continent, which constitute some 0.44% of the total continental land area ...
Max Ortiz, Jason Bosch, Clément Coclet, Jenny Johnson, Pedro Lebre, Adeola Salawu-Rotimi, Surendra Vikram, Thulani Makhalanyane, Don Cowan   +8 more
doaj   +1 more source

Microbial metabolism marvels: a comprehensive review of microbial drug transformation capabilities

Gut microbes
This comprehensive review elucidates the pivotal role of microbes in drug metabolism, synthesizing insights from an exhaustive analysis of over two hundred papers. Employing a structural classification system grounded in drug atom involvement, the review
Filippo Martinelli, Ines Thiele
semanticscholar   +1 more source