Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
Molecular Oncology, EarlyView.We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Measuring the loss of duplicated genes in plant genomes assembled by means of short reads
, 2015 Introduction The assembly of a genome is a complex task, whose hardest step is the resolution of repeats. As these regions are usually considered of minor concern for the description of the features of the genome, they are often poorly characterized in a
De Pascale Fabio +2 more
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The complete chloroplast genome of <i>Epimedium grandiflorum</i> C.Morren 1834 (Berberidaceae), a medicinal plant endemic to Japan. [PDF]
Mitochondrial DNA B ResourZhang BB, Yu FM.
europepmc +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Integrative genomic and phenotypic analyses reveal broad-spectrum antifungal activity and biocontrol potential in Bacillus ayatagriensis. [PDF]
Funct Integr Genomicsde Souza V +7 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The complete chloroplast genome of <i>Lamium purpureum</i> L. var. <i>purpureum</i> (Lamiaceae) and phylogenetic analysis. [PDF]
Mitochondrial DNA B ResourKim YK, Jeon YJ, Song JH.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
, 2017 This internet resource contains reports throughout the yearKansas Cooperative Plant Disease Survey Report Preliminary 2017 Kansas Wheat Loss Estimates -- Highlights ...
Kansas. Plant Protection and Weed Control Section
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Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source