Results 271 to 280 of about 473,506 (307)
Some of the next articles are maybe not open access.
2019
Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.
Lisbeth Ahm, Hansen +1 more
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Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.
Lisbeth Ahm, Hansen +1 more
openaire +2 more sources
2023
The generation of genetically engineered (GE) pigs for disease modeling and xenotransplantation has been massively facilitated by the discovery of the CRISPR/Cas9 system. For livestock, genome editing is a powerful tool when used in combination with either somatic cell nuclear transfer (SCNT) or microinjection (MI) into fertilized oocytes.
David, Preisinger +4 more
openaire +2 more sources
The generation of genetically engineered (GE) pigs for disease modeling and xenotransplantation has been massively facilitated by the discovery of the CRISPR/Cas9 system. For livestock, genome editing is a powerful tool when used in combination with either somatic cell nuclear transfer (SCNT) or microinjection (MI) into fertilized oocytes.
David, Preisinger +4 more
openaire +2 more sources
Prime Editing: A New Way for Genome Editing
Trends in Cell Biology, 2020Precise and efficient use of genome editing tools are hampered by the introduction of DNA double-strand breaks, donor DNA templates, or homology-directed repair. A recent study expands the genome editing toolbox with the introduction of prime editing, which overcomes previous challenges and introduces insertions, deletions, and all putative 12 types of
Hensel, Goetz +2 more
openaire +3 more sources
2017
Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target ...
Satoshi, Ansai, Masato, Kinoshita
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Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target ...
Satoshi, Ansai, Masato, Kinoshita
openaire +2 more sources
2017
Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available and it is possible to cause transgenesis as well as target gene disruption via the genome editing technique. TALEN-mediated knock-out is especially effective in this species.
Takuya, Tsubota, Hideki, Sezutsu
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Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available and it is possible to cause transgenesis as well as target gene disruption via the genome editing technique. TALEN-mediated knock-out is especially effective in this species.
Takuya, Tsubota, Hideki, Sezutsu
openaire +2 more sources
2017
Pigs are important livestock for food and have been used in various biomedical studies, particularly translational research, as experimental animals because of their anatomical and physiological similarity to humans. The recent development of genome editing techniques, such as ZFN, TALEN, and CRISPR/Cas9, has rapidly expanded the use of genome editing ...
Masahito, Watanabe, Hiroshi, Nagashima
openaire +3 more sources
Pigs are important livestock for food and have been used in various biomedical studies, particularly translational research, as experimental animals because of their anatomical and physiological similarity to humans. The recent development of genome editing techniques, such as ZFN, TALEN, and CRISPR/Cas9, has rapidly expanded the use of genome editing ...
Masahito, Watanabe, Hiroshi, Nagashima
openaire +3 more sources
2017
Many genetically engineered rat strains have been produced for biomedical research. The simple and quick production of knock-out and knock-in rats is currently possible using genome editing techniques incorporating zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic ...
openaire +3 more sources
Many genetically engineered rat strains have been produced for biomedical research. The simple and quick production of knock-out and knock-in rats is currently possible using genome editing techniques incorporating zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic ...
openaire +3 more sources
Science, 2009
![Figure][1] CREDIT: ROBERT HAMMERSMITH The ciliate Oxytricha trifallax has an unusual genome with the coding regions of genes (the exons) scattered through the genome. The exons are then somehow knitted together following transcription prior to their translation into proteins.
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![Figure][1] CREDIT: ROBERT HAMMERSMITH The ciliate Oxytricha trifallax has an unusual genome with the coding regions of genes (the exons) scattered through the genome. The exons are then somehow knitted together following transcription prior to their translation into proteins.
openaire +1 more source
2021
There are a multitude of different methods to intervene in the genetic makeup of living beings in an undirected or directed manner. Currently, the CRISPR/Cas system, molecular gene scissors, are causing a sensation, as they make it possible to specifically edit genetic information.
openaire +1 more source
There are a multitude of different methods to intervene in the genetic makeup of living beings in an undirected or directed manner. Currently, the CRISPR/Cas system, molecular gene scissors, are causing a sensation, as they make it possible to specifically edit genetic information.
openaire +1 more source
Regulate genome-edited products, not genome editing itself
Nature Biotechnology, 2016Dana Carroll +4 more
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