Results 371 to 380 of about 1,963,187 (422)
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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Nature Biotechnology, 2020Andrew V Anzalone +2 more
exaly +2 more sources
CRISPR technology: A decade of genome editing is only the beginning
Science, 2023The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era in which genetic diseases and individual disease susceptibilities are ...
Joy Y. Wang, J. Doudna
semanticscholar +1 more source
Advanced Materials, 2021
The potential of the cluster regularly interspaced short palindromic repeat (CRISPR)‐associated protein 9 (Cas9)‐based therapeutic genome editing is severely hampered by the difficulties in precise regulation of the in vivo activity of the CRISPR‐Cas9 ...
Haohao Yin, Yu Chen, Yanling Zheng
exaly +2 more sources
The potential of the cluster regularly interspaced short palindromic repeat (CRISPR)‐associated protein 9 (Cas9)‐based therapeutic genome editing is severely hampered by the difficulties in precise regulation of the in vivo activity of the CRISPR‐Cas9 ...
Haohao Yin, Yu Chen, Yanling Zheng
exaly +2 more sources
2022
Defined as a condition of body fat excess leading to significant morbidity, obesity is a chronic metabolic illness associated with a significant number of diseases. The incidence of obesity does not solely depend on dietary habits, with energy balance being regulated by the complex interactions between genetic, behavioural and environmental factors ...
Masi D., Tozzi R., Watanabe M.
openaire +3 more sources
Defined as a condition of body fat excess leading to significant morbidity, obesity is a chronic metabolic illness associated with a significant number of diseases. The incidence of obesity does not solely depend on dietary habits, with energy balance being regulated by the complex interactions between genetic, behavioural and environmental factors ...
Masi D., Tozzi R., Watanabe M.
openaire +3 more sources
2021
Developed over thousands of years largely through human intervention, the modern maize genome can now be precisely modified for agricultural improvement and scientific research. This chapter focuses on progress made in recent decades utilizing site-specific nuclease (SSN) technologies in maize genome engineering.
Jacob D. Zobrist +5 more
openaire +2 more sources
Developed over thousands of years largely through human intervention, the modern maize genome can now be precisely modified for agricultural improvement and scientific research. This chapter focuses on progress made in recent decades utilizing site-specific nuclease (SSN) technologies in maize genome engineering.
Jacob D. Zobrist +5 more
openaire +2 more sources
2017
Pigs are important livestock for food and have been used in various biomedical studies, particularly translational research, as experimental animals because of their anatomical and physiological similarity to humans. The recent development of genome editing techniques, such as ZFN, TALEN, and CRISPR/Cas9, has rapidly expanded the use of genome editing ...
Masahito Watanabe +3 more
openaire +3 more sources
Pigs are important livestock for food and have been used in various biomedical studies, particularly translational research, as experimental animals because of their anatomical and physiological similarity to humans. The recent development of genome editing techniques, such as ZFN, TALEN, and CRISPR/Cas9, has rapidly expanded the use of genome editing ...
Masahito Watanabe +3 more
openaire +3 more sources
2021
Genetic engineering plays a key role in plant functional research and genetic improvement. A novel and powerful gene editing technique, CRISPR/Cas9, which was developed from a type II bacterial immune system, opened up a new era in precision genetic engineering in plants. This technique is based on a non-permanent transgene system and is starting to be
David R. Holding, Aixia Li
openaire +2 more sources
Genetic engineering plays a key role in plant functional research and genetic improvement. A novel and powerful gene editing technique, CRISPR/Cas9, which was developed from a type II bacterial immune system, opened up a new era in precision genetic engineering in plants. This technique is based on a non-permanent transgene system and is starting to be
David R. Holding, Aixia Li
openaire +2 more sources
Current Opinion in Obstetrics & Gynecology, 2023
Purpose of review The development of modern gene editing tools alongside promising innovations in gene sequencing and prenatal diagnostics as well as a shifting regulatory climate around targeted therapeutics offer an opportunity to address monogenic diseases prior to the onset of pathology. In this review, we seek to highlight
Sourav K, Bose +2 more
openaire +2 more sources
Purpose of review The development of modern gene editing tools alongside promising innovations in gene sequencing and prenatal diagnostics as well as a shifting regulatory climate around targeted therapeutics offer an opportunity to address monogenic diseases prior to the onset of pathology. In this review, we seek to highlight
Sourav K, Bose +2 more
openaire +2 more sources
2021
There are a multitude of different methods to intervene in the genetic makeup of living beings in an undirected or directed manner. Currently, the CRISPR/Cas system, molecular gene scissors, are causing a sensation, as they make it possible to specifically edit genetic information.
openaire +2 more sources
There are a multitude of different methods to intervene in the genetic makeup of living beings in an undirected or directed manner. Currently, the CRISPR/Cas system, molecular gene scissors, are causing a sensation, as they make it possible to specifically edit genetic information.
openaire +2 more sources
2017
Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target ...
Masato Kinoshita +2 more
openaire +3 more sources
Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target ...
Masato Kinoshita +2 more
openaire +3 more sources