Results 311 to 320 of about 526,413 (332)
Some of the next articles are maybe not open access.
IEEE Engineering in Medicine and Biology Magazine, 1999
This work addresses two principles that will be integral to the post-genomic or proteomic era (i.e., after sequencing). The first is that any analysis of data from or related to the Human Genome Project will need to be designed with high-throughput in mind.
M.V. Boland, R.F. Murphy
openaire +1 more source
This work addresses two principles that will be integral to the post-genomic or proteomic era (i.e., after sequencing). The first is that any analysis of data from or related to the Human Genome Project will need to be designed with high-throughput in mind.
M.V. Boland, R.F. Murphy
openaire +1 more source
Genome Engineering Using TALENs
2018Genome engineering involves methods of genetic modification of cells at predefined genomic sites. Here, we used transcription activator-like effector nucleases (TALENs) for the site-directed mutagenesis in barley. Target gene-specific TALEN-encoding expression units were designed and delivered to totipotent cells of either cultivated embryogenic pollen
Goetz, Hensel, Jochen, Kumlehn
openaire +2 more sources
Engineering three-dimensional genome folding
Nature Genetics, 2021Animal genomes are partitioned and folded at various scales that contribute distinctly to nuclear processes. While structural features have been disrupted either globally or at select loci in loss-of-function studies, gain-of-function studies that probe the role of genome architecture have lagged behind.
Di Zhang, Jessica Lam, Gerd A. Blobel
openaire +2 more sources
Multiplexed Genome Engineering with Cas12a
2021Genome engineering technologies based on CRISPR-Cas systems are fueling efforts to study genotype-phenotype relationships in a high-throughput and multiplexed fashion. While many genome engineering technologies exist and provide a means to efficiently manipulate one or a few genes in a singular context-knockout, inhibition, or activation in a ...
Niels R, Weisbach +2 more
openaire +2 more sources
Evolutionary engineering by genome shuffling
Applied Microbiology and Biotechnology, 2014An upsurge in the bioeconomy drives the need for engineering microorganisms with increasingly complex phenotypes. Gains in productivity of industrial microbes depend on the development of improved strains. Classical strain improvement programmes for the generation, screening and isolation of such mutant strains have existed for several decades.
Damien, Biot-Pelletier +1 more
openaire +2 more sources
Programmable genome engineering
Science, 2019Synthetic Biology The model bacterium Escherichia coli has a single circular chromosome. Wang et al. created a method to fragment the E. coli genome into independent chromosomes that can be modified, rearranged, and recombined. The efficient fission of the unmodified E. coli genome into two defined, stable pairs of synthetic chromosomes provides common
openaire +1 more source
1998
• DNA transformation has removed most of the barriers that limit the introduction of specific genes from one organism to another. • The use of Agrobacteria and modified Ti plasmids to transform dicotyledons, and the use of microinjection to transfect the nuclei of animal cells, are being superseded by particle-bombardment biolistic procedures ...
Rudi Appels +3 more
openaire +1 more source
• DNA transformation has removed most of the barriers that limit the introduction of specific genes from one organism to another. • The use of Agrobacteria and modified Ti plasmids to transform dicotyledons, and the use of microinjection to transfect the nuclei of animal cells, are being superseded by particle-bombardment biolistic procedures ...
Rudi Appels +3 more
openaire +1 more source
Genomics and Genetic Engineering
2011The hurricane growth of genomics and genetic engineering poses challenging ethical questions pertaining to the technological application in human life. Many secular and religious bioethicists observe that the new proposals of genetic engineering are described as “playing God.” The metaphor has evoked both optimistic and pessimistic perspectives among ...
openaire +1 more source
Genome Engineering with Custom Recombinases
2014Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications ...
Thomas, Gaj, Carlos F, Barbas
openaire +2 more sources
Genome Engineering in Human Cells
2014Genome editing in human cells is of great value in research, medicine, and biotechnology. Programmable nucleases including zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases recognize a specific target sequence and make a double-strand break at that site, which can result in gene disruption, gene
Minjung, Song +3 more
openaire +2 more sources