Results 161 to 170 of about 1,533,401 (302)

MiniSdd7‐Derived Cytosine Base Editor Promotes Efficient Multiplex Gene Knockout in Porcine Primary Fibroblasts

Animal Research and One Health, EarlyView.
The miniSdd7‐BE4max‐SpG system enables highly efficient multiplex C‐to‐T base editing of APN, CD163, and MSTN in porcine fibroblasts, providing a robust platform for future development of multiplex gene‐edited livestock. ABSTRACT Efficient multiplex genome editing is essential for improving complex traits in livestock that are governed by multiple ...
Shaoshuai Wang, Yu Wang, Dengfeng Bi, Jianguo Zhao, Yanfang Wang   +4 more
wiley   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Genome mining of amylases and amylase inhibitors from <i>Streptomyces</i>. [PDF]

Microb Genom
Lawaetz AC, Gannon A, Hoskisson PA.
europepmc   +1 more source

Genetic and population analyses implicate thyroid‐related regulation of RNF144B in chondrocalcinosis

Arthritis &Rheumatology, Accepted Article.
Objectives Chondrocalcinosis, characterized by calcium crystal deposition within articular cartilage, affects 5–15% of the general population and has recently been identified as an osteoarthritis risk factor. However, Its biological pathways remain unclear.
Yahong Wu, Yanning Xu, Paul C. Okoro, Sirine Saafi, Jard de Vries, Xiaoyi Qi, Edwin H.G. Oei, Sita Bierma‐Zeinstra, Trudy Voortman, David Felson, Joyce BJ van Meurs, Layal Chaker, J. Mark Wilkinson, Michelle S. Yau, Jessica Bertrand, Cindy G. Boer   +15 more
wiley   +1 more source

DiscERN: an automated genome mining tool for the discovery of evolutionarily related natural products. [PDF]

Nat Commun
Owen JG, Woolly EF, Lai HE, Woolner VH, Little RF.   +4 more
europepmc   +1 more source

Early synovial regulatory T cell engagement in posttraumatic osteoarthritis

Arthritis &Rheumatology, Accepted Article.
Objective Posttraumatic osteoarthritis (PTOA) develops despite restoration of joint biomechanics, suggesting that early post‐injury biological processes within the synovium shape disease trajectories. We investigated whether synovial regulatory T (Treg) cells are engaged during the early phase following joint injury and whether experimental enrichment ...
Xueyou Zhang, Mingde Cao, Jianting Li, Bruma Sai‐Chuen Fu, Michael Tim‐Yun Ong, Patrick Shu‐Hang Yung   +5 more
wiley   +1 more source

Antifungal Activity and Genome Mining Reveal Potentiality of Streptomyces sp. VNUA24 as a Biocontrol Agent. [PDF]

Plant Pathol J
Dang TTT, Nguyen TT, Nguyen GV, Dinh ST, Tran DT, Nguyen CX.   +5 more
europepmc   +1 more source

Immunoinformatics‐Based Design of a Multi‐Epitope Vaccine Targeting a Conserved Copper–Associated Protein in Neisseria gonorrhoeae

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Neisseria gonorrhoeae poses an urgent public health challenge due to rapidly increasing antimicrobial resistance and the absence of an effective vaccine. Targeting conserved bacterial pathways involved in essential physiological processes may provide new opportunities for vaccine antigen discovery.
Sinethemba H. Yakobi, Uchechukwu U. Nwodo   +1 more
wiley   +1 more source

A Machine Learning-Based Genome Mining Approach Reveals Unprecedented Biarylitide Diversity. [PDF]

JACS Au
Padva L, Gullick J, Biermann F, Coe LJ, Zhao Y, Tucker S, Zimmer L, Tailhades J, Kehraus S, Schittenhelm RB, De Voss JJ, Helfrich EJN, Cryle MJ, Crüsemann M.   +13 more
europepmc   +1 more source

Limestone Mining - Is It Noisy Or Not? [PDF]

National Institute for Occupational Safety and Health (NIOSH) researchers are conducting a cross-sectional survey of equipment noise and worker noise exposures in the mining industry.

core