Results 1 to 10 of about 4,220,889 (382)

Microfluidic purification of genomic DNA. [PDF]

Proc Natl Acad Sci U S A
We describe a microfluidic device to extract DNA from a cell lysate, without the need for centrifuges, magnetic beads, or gels. Instead, separation is driven by transverse migration of DNA, which occurs when a polyelectrolyte solution flowing through a microfluidic channel is subjected to an electric field.
Wang J, Butler JE, Ladd AJC.
europepmc   +3 more sources

Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing [PDF]

Microorganisms
Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation of ultra-high-molecular-weight genomic DNA of sufficient quality and length for error-free third-generation sequencing remains challenging.
Ayşe Öykü Ova, Enrique Joffre, Reza Zandi Shafagh, Mariana F. G. Assunção, Roman Y. Sidorov, Lilia M. A. Santos, Volker M. Lauschke, Ute Römling   +7 more
doaj   +2 more sources

Sequencing of Genomic DNA [PDF]

BioTechniques, 1999
Ulrich Mahlknecht, Dieter Hoelzer, Richard Bucala   +2 more
doaj   +4 more sources

A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

Proceedings of the National Academy of Sciences of the United States of America, 1992
Marianne Frommer, Louise E. McDonald, David Millar, Christina M. Collis, Fujiko Watt, G. W. GRIGG, Peter L. Molloy, Cheryl Paul   +7 more
openalex   +2 more sources

Whole-Exome Sequencing Identifies a Novel POLG Frameshift Variant in an Adult Patient Presenting with Progressive External Ophthalmoplegia and Mitochondrial DNA Depletion

Case Reports in Genetics, 2021
Mitochondrial DNA (mtDNA) depletion syndromes are a group of autosomal recessive disorders associated with a spectrum of clinical diseases, which include progressive external ophthalmoplegia (PEO).
Justin Kurtz, Joseph Americo Fernandes, Mahesh Mansukhani, William C. Copeland, Ali B. Naini   +4 more
doaj   +1 more source

In Vitro Isolation and Molecular Characterization of Genomic DNA from Ancient Human Long and Hip Bones

MAMC Journal of Medical Sciences, 2023
Background: A simple and effective modified ethanol precipitation-based protocol was described for the extraction of genomic DNA from ancient human bones.
Dinesh Kumar, Lalit Mohan Jeena, Devendra Shekhawat, Ira Verma, Sidd Sana   +4 more
doaj   +1 more source

Remodeling the genome with DNA twists [PDF]

Science, 2019
Intricate protein machines repackage DNA to turn genes on and ...
Bowman, Gregory D., Deindl, Sebastian
openaire   +3 more sources

Inhibition of the PCR by genomic DNA

PLOS ONE, 2023
Aims qPCR, is widely used for quantifying minimal residual disease (MRD) and is conventionally performed according to guidelines proposed by the EuroMRD consortium. However it often fails when quantifying MRD levels below 10−4. By contrast, HAT-PCR, a recent modification designed to minimise false-positive results, can quantify MRD down to 10−6 ...
Sue Latham, Elizabeth Hughes, Bradley Budgen, Alexander Morley   +3 more
openaire   +3 more sources

G4 DNA: at risk in the genome [PDF]

The EMBO Journal, 2011
Regions bearing G‐quadruplex (G4) DNA motifs can be sites of genomic instability and are frequently depleted in streamlined genomes, but are nevertheless maintained in many other genomes. Whether G4 secondary structures form in vivo , and how they are maintained or eliminated, remains little known.
Nancy Maizels, Luther Davis
openaire   +3 more sources

An alternative application of some SSR DNA markers in experimental mycology

Ukrainian Botanical Journal, 2023
The expediency of using unique SSR DNA-markers of Schizophyllum commune for population genetic assays in various fungal species has been demonstrated.
Boiko S.M.
doaj   +1 more source