Results 1 to 10 of about 1,136,601 (292)

Viral Genomic DNA Packaging Machinery. [PDF]

Subcell Biochem
Tailed double-stranded DNA bacteriophage employs a protein terminase motor to package their genome into a preformed protein shell-a system shared with eukaryotic dsDNA viruses such as herpesviruses. DNA packaging motor proteins represent excellent targets for antiviral therapy, with Letermovir, which binds Cytomegalovirus terminase, already licensed as
Hawkins DEDP, Godwin OC, Antson AA.
europepmc   +4 more sources

Microfluidic purification of genomic DNA. [PDF]

Proc Natl Acad Sci U S A
We describe a microfluidic device to extract DNA from a cell lysate, without the need for centrifuges, magnetic beads, or gels. Instead, separation is driven by transverse migration of DNA, which occurs when a polyelectrolyte solution flowing through a microfluidic channel is subjected to an electric field.
Wang J, Butler JE, Ladd AJC.
europepmc   +3 more sources

Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing [PDF]

Microorganisms
Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation of ultra-high-molecular-weight genomic DNA of sufficient quality and length for error-free third-generation sequencing remains challenging.
Ayşe Öykü Ova, Enrique Joffre, Reza Zandi Shafagh, Mariana F. G. Assunção, Roman Y. Sidorov, Lilia M. A. Santos, Volker M. Lauschke, Ute Römling   +7 more
doaj   +2 more sources

Sequencing of Genomic DNA [PDF]

BioTechniques, 1999
Ulrich Mahlknecht, Dieter Hoelzer, Richard Bucala   +2 more
doaj   +3 more sources

Whole-Exome Sequencing Identifies a Novel POLG Frameshift Variant in an Adult Patient Presenting with Progressive External Ophthalmoplegia and Mitochondrial DNA Depletion

Case Reports in Genetics, 2021
Mitochondrial DNA (mtDNA) depletion syndromes are a group of autosomal recessive disorders associated with a spectrum of clinical diseases, which include progressive external ophthalmoplegia (PEO).
Justin Kurtz, Joseph Americo Fernandes, Mahesh Mansukhani, William C. Copeland, Ali B. Naini   +4 more
doaj   +1 more source

In Vitro Isolation and Molecular Characterization of Genomic DNA from Ancient Human Long and Hip Bones

MAMC Journal of Medical Sciences, 2023
Background: A simple and effective modified ethanol precipitation-based protocol was described for the extraction of genomic DNA from ancient human bones.
Dinesh Kumar, Lalit Mohan Jeena, Devendra Shekhawat, Ira Verma, Sidd Sana   +4 more
doaj   +1 more source

Analysis of DNA isolated from different oil sources: problems and solution [PDF]

Iranian Journal of Veterinary Medicine, 2017
Background: One of the major aspects of traceability in food authenticity assessment is to explore practical methods to find the origin of food. Objective: The aim of the present study was to find a DNA based method for authentication and traceability ...
Parviz shayan, Ghazal Nemati, Abolfazl Kamkar, Brigitte Eckert, Afshin Akhondzadeh Basti, Negin Nouri, Iraj Ashrafi Tamai1   +6 more
doaj   +1 more source

An alternative application of some SSR DNA markers in experimental mycology

Ukrainian Botanical Journal, 2023
The expediency of using unique SSR DNA-markers of Schizophyllum commune for population genetic assays in various fungal species has been demonstrated.
Boiko S.M.
doaj   +1 more source

Novel compound heterozygous stop‐gain mutations of LRBA in a Vietnamese patient with Common Variable Immune Deficiency

Molecular Genetics & Genomic Medicine, 2020
Background Lipopolysaccharide‐responsive and beige‐like anchor (LRBA) deficiency is a rare autosomal recessive common variable immunodeficiency (CVID), affecting 1:25,000–1:50,000 people worldwide.
Anh N. L. Phan, Thuy T. T. Pham, Nghia Huynh, Tuan M. Nguyen, Cuc T. T. Cao, Duong T. Nguyen, Duc T. Le, Chi‐Bao Bui   +7 more
doaj   +1 more source

Metal-Binding Antimicrobial Peptide SIF4 Kills Escherichia coli by Targeting Cytoplasmic Biomacromolecules without Cytoplasmic Membrane Damage: A Mechanistic Study [PDF]

Shipin Kexue, 2023
To explore how metal-binding antimicrobial peptide SIF4 kills foodborne Escherichia coli by targeting nucleic acid and protein in the cytoplasmic membrane without cytoplasmic membrane damage, the effect of SIF4 on intracellular nucleic acid biosynthesis ...
LI Yuzhen, XIAO Huaiqiu, LIU Miao, WANG Lin, ZENG Mengqi, ZHAO Mouming
doaj   +1 more source