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Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing [PDF]

Microorganisms
Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation of ultra-high-molecular-weight genomic DNA of sufficient quality and length for error-free third-generation sequencing remains challenging.
Ayşe Öykü Ova, Enrique Joffre, Reza Zandi Shafagh, Mariana F. G. Assunção, Roman Y. Sidorov, Lilia M. A. Santos, Volker M. Lauschke, Ute Römling   +7 more
doaj   +2 more sources

A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

Proceedings of the National Academy of Sciences of the United States of America, 1992
Marianne Frommer, Louise E. McDonald, David Millar, Christina M. Collis, Fujiko Watt, G. W. GRIGG, Peter L. Molloy, Cheryl Paul   +7 more
openalex   +2 more sources

Whole-Exome Sequencing Identifies a Novel POLG Frameshift Variant in an Adult Patient Presenting with Progressive External Ophthalmoplegia and Mitochondrial DNA Depletion

Case Reports in Genetics, 2021
Mitochondrial DNA (mtDNA) depletion syndromes are a group of autosomal recessive disorders associated with a spectrum of clinical diseases, which include progressive external ophthalmoplegia (PEO).
Justin Kurtz, Joseph Americo Fernandes, Mahesh Mansukhani, William C. Copeland, Ali B. Naini   +4 more
doaj   +1 more source

In Vitro Isolation and Molecular Characterization of Genomic DNA from Ancient Human Long and Hip Bones

MAMC Journal of Medical Sciences, 2023
Background: A simple and effective modified ethanol precipitation-based protocol was described for the extraction of genomic DNA from ancient human bones.
Dinesh Kumar, Lalit Mohan Jeena, Devendra Shekhawat, Ira Verma, Sidd Sana   +4 more
doaj   +1 more source

The Adaptive Significance of Natural Genetic Variation in the DNA Damage Response of Drosophila melanogaster. [PDF]

, 2016
Despite decades of work, our understanding of the distribution of fitness effects of segregating genetic variants in natural populations remains largely incomplete. One form of selection that can maintain genetic variation is spatially varying selection,
Begun, David J, Cridland, Julie M, Svetec, Nicolas, Zhao, Li   +3 more
core   +12 more sources

An alternative application of some SSR DNA markers in experimental mycology

Ukrainian Botanical Journal, 2023
The expediency of using unique SSR DNA-markers of Schizophyllum commune for population genetic assays in various fungal species has been demonstrated.
Boiko S.M.
doaj   +1 more source

Analysis of DNA isolated from different oil sources: problems and solution [PDF]

Iranian Journal of Veterinary Medicine, 2017
Background: One of the major aspects of traceability in food authenticity assessment is to explore practical methods to find the origin of food. Objective: The aim of the present study was to find a DNA based method for authentication and traceability ...
Parviz shayan, Ghazal Nemati, Abolfazl Kamkar, Brigitte Eckert, Afshin Akhondzadeh Basti, Negin Nouri, Iraj Ashrafi Tamai1   +6 more
doaj   +1 more source

Preparation of enteroinvasive Escherichia coli reference materials for food analysis

Zhongguo shipin weisheng zazhi, 2021
ObjectiveTo satisfy the demands of laboratory quality control and proficiency testing for the detection of enteroinvasive Escherichia coli (EIEC), a stable EIEC reference material was developed with independent intellectual property rights, good ...
ZHAO Linna, WANG Xueshuo, LIU Na, CUI Shenghui, LU Yong   +4 more
doaj   +1 more source

Preliminary study of familial nonsyndromic tooth agenesis caused by ectodysplasin A mutation

口腔疾病防治, 2023
Objective To explore the pathogenic genes in a Chinese family affected by nonsyndromic tooth agenesis so as to study the pathogenesis of oligodontia. Methods Hospital ethical approval and informed consent of the patients and family members were obtained.
WANG Huihui, WU Qing , XU Bin, LING Qi , WU Yiqun   +4 more
doaj   +1 more source

Novel compound heterozygous stop‐gain mutations of LRBA in a Vietnamese patient with Common Variable Immune Deficiency

Molecular Genetics & Genomic Medicine, 2020
Background Lipopolysaccharide‐responsive and beige‐like anchor (LRBA) deficiency is a rare autosomal recessive common variable immunodeficiency (CVID), affecting 1:25,000–1:50,000 people worldwide.
Anh N. L. Phan, Thuy T. T. Pham, Nghia Huynh, Tuan M. Nguyen, Cuc T. T. Cao, Duong T. Nguyen, Duc T. Le, Chi‐Bao Bui   +7 more
doaj   +1 more source