Results 261 to 270 of about 1,136,601 (292)
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Orthopoxvirus pan-genomic DNA assay
Journal of Virological Methods, 2007A genome-spanning assay is described that enables laboratory confirmation of infections with orthopoxviruses (OPVs), particularly Vaccinia, Monkeypox, and Variola viruses, which can cause vesiculo-pustular rash illnesses in humans. The assay uses a series of polymerase chain reaction (PCR) amplicons that overlap to span the approximately 200kilobase ...
Yu, Li +4 more
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Repetitive DNA in eukaryotic genomes
Chromosome Research, 2015Repetitive DNA--sequence motifs repeated hundreds or thousands of times in the genome--makes up the major proportion of all the nuclear DNA in most eukaryotic genomes. However, the significance of repetitive DNA in the genome is not completely understood, and it has been considered to have both structural and functional roles, or perhaps even no ...
Maria Assunta, Biscotti +2 more
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1997
Abstract There are several methods for extracting genomic DNA from tissue culture cells. Presented here are two methods: a relatively new method which is’very simple, quick and convenient (no phenol) and a reliable, although perhaps outdated (i.e., phenol/chloroform), method. Genomic DNA can be extracted from either tissue culture cells (
Raul M Torres, Ralf Kühn
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Abstract There are several methods for extracting genomic DNA from tissue culture cells. Presented here are two methods: a relatively new method which is’very simple, quick and convenient (no phenol) and a reliable, although perhaps outdated (i.e., phenol/chloroform), method. Genomic DNA can be extracted from either tissue culture cells (
Raul M Torres, Ralf Kühn
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Genome‐wide DNA methylation profiling
WIREs Systems Biology and Medicine, 2009AbstractDNA methylation plays a critical role in the regulation of gene expression. The ability to access the methylation status for a large number of genes or the entire genome should greatly facilitate the understanding of the nature of gene regulation in cells, and epigenetic mechanism of interactions between cells and environment.
Marina, Bibikova, Jian-Bing, Fan
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Secret Signatures Inside Genomic DNA
Biotechnology Progress, 2004A simple, practical method to watermark short trademarks or signatures into genomic DNA is introduced. Since the marking method is biologically innocuous, it can be applied to all commercialized bacteria to help establish brand names for the engineered strains and to resolve legal disputes regarding gene-related patents.
Masanori, Arita, Yoshiaki, Ohashi
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Genome: twisting stories with DNA
Endeavour, 2013In 1920, the German botanist Hans Winkler coined the concept of the 'genome'. This paper explores the history of a concept that has developed in parallel with advances in biology and supports novel and powerful heuristic biological research in the 21st century.
Ricardo, Noguera-Solano +2 more
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Genome Size of Mycoplasmal DNA
Nature, 1969ELECTRON microscopic studies of the contour length of DNA from a mycoplasma species, Mycoplasma hominis (H 39)1, have shown that the DNA in this organism is organized in a single circular chromosome, 262 microns long, corresponding to a molecular weight of 5.0 × 108 daltons.
A L, Bak +3 more
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Symbiotic DNA in eukaryotic genomes
Trends in Ecology & Evolution, 1996The recent explosive growth of molecular genetic databases has yielded increasingly detailed insights into the evolutionary dynamics of eukaryotic genomes. DNA sequences with the self-encoded ability to transpose and replicate are unexpectedly abundant and widespread in eukaryotic genomes. They seem to be sexual parasites.
C, Zeyl, G, Bell
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1998
1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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DNA clustering and genome complexity
Computational Biology and Chemistry, 2014Early global measures of genome complexity (power spectra, the analysis of fluctuations in DNA walks or compositional segmentation) uncovered a high degree of complexity in eukaryotic genome sequences. The main evolutionary mechanisms leading to increases in genome complexity (i.e.
Francisco, Dios +5 more
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