Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Nature, 2016 Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target locus as the first step to gene correction. Although most genetic diseases arise from point mutations, current approaches to point mutation correction are ...
Alexis C. Komor +4 more
semanticscholar +1 more source
Genomic instability in human cancer: molecular insights and opportunities for therapeutic attack and prevention through diet and nutrition [PDF]
, 2015 Genomic instability can initiate cancer, augment progression, and influence the overall prognosis of the affected patient. Genomic instability arises from many different pathways, such as telomere damage, centrosome amplification, epigenetic ...
Amedei, Amedeo +34 more
core +1 more source
Prediction of complete gene structures in human genomic DNA.
Journal of Molecular Biology, 1997 We introduce a general probabilistic model of the gene structure of human genomic sequences which incorporates descriptions of the basic transcriptional, translational and splicing signals, as well as length distributions and compositional features of ...
C. Burge, S. Karlin
semanticscholar +1 more source
DNA Checkpoint and Repair Factors Are Nuclear Sensors for Intracellular Organelle Stresses-Inflammations and Cancers Can Have High Genomic Risks. [PDF]
, 2018 Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability.
Chen, Hongping +21 more
core +2 more sources
Not seeing the genomes for the DNA [PDF]
Briefings in Functional Genomics, 2012 This Short Communication highlights the diversity of 'secondary' genome data (like mitochondrial and plastid genomes) that can be gleaned from next-generation sequencing projects, and encourages researchers to be mindful that these data are often as informative and useful as the 'primary' genome data.
openaire +3 more sources
The complete mitochondrial genome of Cepola schlegelii from the East China Sea
Mitochondrial DNA. Part B. Resources, 2022 Cepola schlegelii (Bleeker 1854) belongs to the genus Cepola in the family Cepolidae and order Priacanthiformes. The complete mitochondrial genome of C. schlegelii was sequenced and analyzed by a high-throughput sequencing approach.
Panjiao Liang +5 more
doaj +1 more source
Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.
Nucleic Acids Research, 1997 A very simple, fast, universally applicable and reproducible method to extract high quality megabase genomic DNA from different organisms is described. We applied the same method to extract high quality complex genomic DNA from different tissues (wheat ...
S. Aljanabi, Iciar Martinez
semanticscholar +1 more source
Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage. [PDF]
, 2016 The hormone estrogen (E2) binds the estrogen receptor to promote transcription of E2-responsive genes in the breast and other tissues. E2 also has links to genomic instability, and elevated E2 levels are tied to breast cancer.
Bocek, Michael +7 more
core +3 more sources
eLife, 2014 Homologous recombination (HR)-mediated repair of DNA double-strand break (DSB)s is restricted to the post-replicative phases of the cell cycle. Initiation of HR in the G1 phase blocks non-homologous end joining (NHEJ) impairing DSB repair.
Young Eun Choi +6 more
doaj +1 more source
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.
Nucleic Acids Research, 1991 The polymerase chain reaction (PCR) has revolutionised the rapid analysis of mammalian genomic DNA (1). However, PCR is less useful in the analysis of plant DNA due to the difficulties in extracting nucleic acids from limited amounts of plant tissue.
K. Edwards, C. Johnstone, C. Thompson
semanticscholar +1 more source