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DNA clustering and genome complexity
Computational Biology and Chemistry, 2014Early global measures of genome complexity (power spectra, the analysis of fluctuations in DNA walks or compositional segmentation) uncovered a high degree of complexity in eukaryotic genome sequences. The main evolutionary mechanisms leading to increases in genome complexity (i.e.
Guillermo Barturen +5 more
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Genome: twisting stories with DNA
Endeavour, 2013In 1920, the German botanist Hans Winkler coined the concept of the 'genome'. This paper explores the history of a concept that has developed in parallel with advances in biology and supports novel and powerful heuristic biological research in the 21st century.
Ricardo Noguera-Solano +2 more
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1998
1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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The Genomics of Plant Satellite DNA
2021The twenty-first century began with a certain indifference to the research of satellite DNA (satDNA). Neither genome sequencing projects were able to accurately encompass the study of satDNA nor classic methodologies were able to go further in undertaking a better comprehensive study of the whole set of satDNA sequences of a genome.
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1997
It has been claimed that there exist at least as many methods of isolating nudeic acids as there are molecular biology laboratories (Jowett, 1986). This is surely an understatement, since my experience tells me that every graduate student will tend to introduce a new, usually irrelevant modification that, he or she hopes, will bring him to the top of ...
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It has been claimed that there exist at least as many methods of isolating nudeic acids as there are molecular biology laboratories (Jowett, 1986). This is surely an understatement, since my experience tells me that every graduate student will tend to introduce a new, usually irrelevant modification that, he or she hopes, will bring him to the top of ...
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Current Biology, 1994
The extremely high AT content of bat DNA complicates the reconstruction of bat phylogeny from DNA sequence data, but may help throw light on genomic evolution.
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The extremely high AT content of bat DNA complicates the reconstruction of bat phylogeny from DNA sequence data, but may help throw light on genomic evolution.
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DNA Methylation and Mycoplasma Genomes
Journal of Molecular Evolution, 2003DNA methylation is one of the many hypotheses proposed to explain the observed deficiency in CpG dinucleotides in a variety of genomes covering a wide taxonomic distribution. Recent studies challenged the methylation hypothesis on empirical grounds. First, it cannot explain why the Mycoplasma genitalium genome exhibits strong CpG deficiency without DNA
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1999
The genome of plant DNA viruses can be either single-stranded or double-stranded. Family Geminiviridae (Mayo and Pringle, 1998; geminiviruses) and the proposed genus Nanovirus (Pringle, 1998; Nanoviruses) contain single-stranded DNA (ssDNA) genome while family Caulimoviridae (Mayo and Pringle, 1998) contains double-stranded DNA (dsDNA) genome ...
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The genome of plant DNA viruses can be either single-stranded or double-stranded. Family Geminiviridae (Mayo and Pringle, 1998; geminiviruses) and the proposed genus Nanovirus (Pringle, 1998; Nanoviruses) contain single-stranded DNA (ssDNA) genome while family Caulimoviridae (Mayo and Pringle, 1998) contains double-stranded DNA (dsDNA) genome ...
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The origin of DNA genomes and DNA replication proteins
Current Opinion in Microbiology, 2002In recent years, it has became clear that most proteins involved in cellular DNA precursor synthesis or DNA replication have been 'invented' more than once, indicating that the transition from RNA to DNA genomes was more complex than previously thought.
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Amplification of Genomic DNA by PCR
2003The polymerase chain reaction (PCR) is used to amplify a segment of DNA that lies between two regions of known sequence (1-3). It requires two oligonucleotide primers that flank the DNA fragment to be amplified and employs repeated cycles of heat denaturation of the DNA, annealing of the primers to their complementary sequences, and extension of the ...
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