Results 91 to 100 of about 596,095 (346)

Plant Sequence Capture Optimised for Illumina Sequencing

open access: yesBio-Protocol, 2014
Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013).
Axel Himmelbach   +2 more
doaj   +1 more source

A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in Mycobacterium abscessus

open access: yesmSystems, 2020
We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and ...
Mark Foreman   +2 more
doaj   +1 more source

A PCR-based method for isolation of genomic DNA flanking a known DNA sequence [PDF]

open access: yes, 1999
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known DNA sequence. The method is based on the directional cloning of digested genomic DNA into the multiple cloning site of a pUC-based plasmid to generate a ...
Boulter, CA, Natarajan, D
core  

Cell‐free DNA aneuploidy score as a dynamic early response marker in prostate cancer

open access: yesMolecular Oncology, EarlyView.
mFast‐SeqS‐based genome‐wide aneuploidy scores are concordant with aneuploidy scores obtained by whole genome sequencing from tumor tissue and can predict response to ARSI treatment at baseline and, at an early time point, to ARSI and taxanes. This assay can be easily performed at low cost and requires little input of cfDNA. Cell‐free circulating tumor
Khrystany T. Isebia   +17 more
wiley   +1 more source

Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

open access: yesBMC Genomics, 2007
Background Giant panda is rare and endangered species endemic to China. The low rates of reproductive success and infectious disease resistance have severely hampered the development of captive and wild populations of the giant panda.
Pan Hui-Juan   +5 more
doaj   +1 more source

Theoretical Bounds on Mate-Pair Information for Accurate Genome Assembly [PDF]

open access: yesarXiv, 2013
Over the past two decades, a series of works have aimed at studying the problem of genome assembly: the process of reconstructing a genome from sequence reads. An early formulation of the genome assembly problem showed that genome reconstruction is NP-hard when framed as finding the shortest sequence that contains all observed reads.
arxiv  

100K Pathogen Genome Project. [PDF]

open access: yes, 2017
The 100K Pathogen Genome Project is producing draft and closed genome sequences from diverse pathogens. This project expanded globally to include a snapshot of global bacterial genome diversity.
Weimer, Bart C
core   +1 more source

Adaptation of a commercial robot for genome library replication [PDF]

open access: yes, 1994
This report describes tools and fixtures developed at the Human Genome Center at Lawrence Berkeley Laboratory for the Hewlett-Packard ORCA{trademark} (Optimized Robot for Chemical Analysis) to replicate large genome libraries. Photographs and engineering drawings of the various custom-designed components are included.
Uber, D.C., Searles, W.L.
openaire   +3 more sources

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

open access: yesMolecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin   +14 more
wiley   +1 more source

Screening of Genomic Library of Soybean Cultivar Lumut by Using Peroxidase Gene from Arabidopsis thaliana as Probe

open access: yesJurnal Ilmu Dasar, 2010
Screening to genomic library of soybean cv. Lumut by using gene encoding for peroxidase (per) from A. thaliana as a probe has an objective isolate the whole gene of per from soybean.
Suharsono Suharsono   +2 more
doaj  

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