BMC Genomics, 2008 Background Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its first generation linkage map has been applied to map QTL for growth traits.
Lin Grace +7 more
doaj +1 more source
Deionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli. [PDF]
Microorganisms, 2023 Yang SK +5 more
europepmc +1 more source
Plasmid Library Construction From Genomic DNA
Current ProtocolsAbstractFunctional genomic approaches have been effective at uncovering the function of uncharacterized genes and identifying new functions for known genes. Often these approaches rely on an in vivo screen or selection to associate genes with a phenotype of interest.
Valeria Florez‐Cardona +4 more
openaire +3 more sources
Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. [PDF]
, 2019 Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated.
Aradhana +20 more
core
ATF4‐mediated stress response as a therapeutic vulnerability in chordoma
Molecular Oncology, EarlyView.We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone +11 more
wiley +1 more source
Construction of Genomic Library and Screening of Edwardsiella tarda Immunogenic Proteins for Their Protective Efficacy Against Edwardsiellosis. [PDF]
Front Immunol, 2021 Bothammal P +6 more
europepmc +1 more source
Molecular Oncology, EarlyView.This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris +10 more
wiley +1 more source
Analysis of genomic regions of Trichoderma harzianum IOC-3844 related to biomass degradation.
PLoS ONE, 2015 Trichoderma harzianum IOC-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications in second-generation bioethanol production. However, the T.
Aline Crucello +9 more
doaj +1 more source
Making genome browsers portable and personal
Genome Biology, 2018 GIVE is a framework and library for creating portable and personalized genome browsers. It makes visualizing genomic data as easy as building a laboratory homepage.
Aziz Khan, Xuegong Zhang
doaj +1 more source
A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA. [PDF]
J Hered, 2021 Kapp JD, Green RE, Shapiro B.
europepmc +1 more source