Results 111 to 120 of about 633,728 (345)

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Heteroduplex Resolution Using T7 Endonuclease I in Microbial Community Analyses

BioTechniques, 2000
Microbial community analyses using molecular techniques, such as PCR followed by genomic library construction, have been helpful in better understanding microbial communities.
Jennifer L. Lowell, Donald A. Klein
doaj   +1 more source

Are personalities genetically determined? Inferences from subsocial spiders. [PDF]

, 2019
BACKGROUND:Recent research has revealed that polymorphic behavioral strategies shape intra-and interspecific interactions and contribute to fitness in many animal species.
Pruitt, Jonathan N, Purcell, Jessica
core  

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

High-throughput PacBio library preparation and sequencing techniques for genomic DNA and TNA

Frontiers in Genetics
PacBio Sequencing is an effective tool for achieving long read lengths to close gaps in whole genome assemblies. Limited information is available for automated, high-throughput library preparation workflows for PacBio sequencing. This paper describes two
Meghan L. Bentz, Cristina Clines, Jasmine Padilla, Christopher Horn, Justin Lee, Mili Sheth   +5 more
doaj   +1 more source

The highest-copy repeats are methylated in the small genome of the early divergent vascular plant Selaginella moellendorffii

BMC Genomics, 2008
Background The lycophyte Selaginella moellendorffii is a vascular plant that diverged from the fern/seed plant lineage at least 400 million years ago. Although genomic information for S.
Quan Hui, Buckley Stephanie, O'Brien Kimberly, Melake-Berhan Admasu, Chan Agnes P, Chen Dan, Lewis Matthew, Banks Jo, Rabinowicz Pablo D   +8 more
doaj   +1 more source

Mapping Genomic Library Clones Using Oligonucleotide Arrays

Genomics, 1996
We have developed a high-density DNA probe array and accompanying biochemical and informatic methods to order clones from genomic libraries. This approach involves a series of enzymatic steps for capturing a set of short dispersed sequence markers scattered throughout a high-molecular-weight DNA.
R J, Sapolsky, R J, Lipshutz
openaire   +2 more sources

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species

Biologia Plantarum, 2011
Jatropha curcas L. (physic nut) is native to Central America and now naturalized widely in many tropical and subtropical areas. Microsatellite markers were isolated and characterized.
C. Phumichai, T. Phumichai, N. Kongsiri, A. Wongkaew, P. Sripichit, R. Kaveeta   +5 more
doaj   +1 more source

RGC1/RGC2 deletions cause increased sensitivity to oxidative stress in Saccharomyces cerevisiae, which can be overcome by constitutive nuclear Yap1 expression [PDF]

, 2016
Oxidative stress mechanism in yeast presents an innovative pathway to understand in creating the next generation of antifungal drugs. Rgc1 and Rgc2 are paralogous proteins that regulate the Fps1 glycerol channel in hyperosmotic stress.
Tsai, Michelle
core   +1 more source