Pooled library screening with multiplexed Cpf1 library
Nature Communications, 2019 AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.
Jintan Liu +11 more
doaj +1 more source
SlopMap: a software application tool for quick and flexible identification of similar sequences using exact k-mer matching [PDF]
arXiv, 2013 With the advent of Next-Generation (NG) sequencing, it has become possible to sequence an entire genome quickly and inexpensively. However, in some experiments one only needs to extract and assembly a portion of the sequence reads, for example when performing transcriptome studies, sequencing mitochondrial genomes, or characterizing exomes.
arxiv
Analysis of a genomic DNA expression library of Mycobacterium tuberculosis using tuberculosis patient sera: evidence for modulation of host immune response [PDF]
, 1996 Rama Rao Amara, Vijaya Satchidanandam
openalex +1 more source
FGF14 GAA Intronic Expansion in Unsolved Adult‐Onset Ataxia in the Care4Rare Canada Consortium
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Background and Objectives Spinocerebellar ataxias (SCA) represent a clinically and genetically heterogeneous group of progressive neurodegenerative diseases with prominent cerebellar atrophy. Recently, a novel pathogenic repeat expansion in intron 1 of FGF14 was identified, causing adult‐onset SCA (SCA27B). We aimed to determine the proportion
Alexanne Cuillerier +20 more
wiley +1 more source
Unleashing the Power of Multiomics: Unraveling the Molecular Landscape of Peripheral Neuropathy
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Peripheral neuropathies (PNs) affect over 20 million individuals in the United States, manifesting as a wide range of sensory, motor, and autonomic nerve symptoms. While various conditions such as diabetes, metabolic disorders, trauma, autoimmune disease, and chemotherapy‐induced neurotoxicity have been linked to PN, approximately one‐third of
Julie Choi +7 more
wiley +1 more source
Construction of male and female PAC genomic libraries suitable for identification of Y‐chromosome‐specific clones from the liverwort, Marchantia polymorpha [PDF]
, 2000 Sachiko Okada +13 more
openalex +1 more source
Ontology‐Based Digital Infrastructure for Data‐Driven Glass Development
Advanced Engineering Materials, EarlyView.This work addresses the inefficiencies in traditional glass development by implementing an ontology‐based digital infrastructure coupled with a high‐throughput robotic melting system. The approach integrates machine learning models, predictive tools, and a semantic database.
Ya‐Fan Chen +20 more
wiley +1 more source
Differentiation of strains of Giardia intestinalis by identification of restriction fragment length polymorphims (RFLP's) and the construction of a gene library [microform] : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University [PDF]
, 1993 Irregular Pagination – Page 26 Vault copy checked. Misnumbered.Giardia intestinalis is a flagellate protozoan which infects the gastrointestinal tract of humans and other mammals such as cats, dogs and farm animals.
Campbell, Shalome Anitta
core
Rapid cloning ofmetKencoding methionine adenosyltransferase fromCorynebacterium glutamicumby screening a genomic library on a high density colony-array [PDF]
, 2000 Kerstin GroÃmann +2 more
openalex +1 more source
Advanced Functional Materials, EarlyView.Designer DNA Architecture‐templated Drug Conjugates (DDA‐DCs) are comprised of a DNA nanomaterial decorated with multiple drug‐loaded DNA aptamers. DDA‐DCs use a combination of multiple aptamer types, each recognizing a different membrane protein to achieve precise targeting of cancer cells.
Abhisek Dwivedy +19 more
wiley +1 more source