Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
core +2 more sources
Characterization of Mauritius parakeet (Psittacula eques) microsatellite loci and their cross-utility in other parrots (Psittacidae, Aves). [PDF]
, 2009 We characterized 21 polymorphic microsatellite loci in the endangered Mauritius parakeet (Psittacula eques). Loci were isolated from a Mauritius parakeet genomic library that had been enriched separately for eight different repeat motifs.
Dawson, Deborah A. +4 more
core +1 more source
CRISPR SWAPnDROP -- A multifunctional system for genome editing and large-scale interspecies gene transfer [PDF]
, 2021 The need for diverse chromosomal modifications in biotechnology, synthetic biology and basic research requires the development of new technologies. With CRISPR SWAPnDROP, we extend the limits of genome editing to large-scale in-vivo DNA transfer between bacterial species.
arxiv +1 more source
Preparation of Genomic Overexpression Library
Bio-Protocol, 2012 This protocol is used to identify/select for Escherichia coli genes that, when overexpressed in Vibrio cholerae Hsp33 (hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage.
Wei-Yun Wholey
doaj +1 more source
EGC: a format for expressing prokaryotic genomes content expectations [PDF]
arXiv, 2023 The number of available genomes of prokaryotic organisms is rapidly growing enabling comparative genomics studies. The comparison of genomes of organisms with a common phenotype, habitat or phylogeny often shows that these genomes share some common contents.
arxiv
Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]
PLoS ONE, 2010 BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann +4 more
doaj +1 more source
Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp [PDF]
, 2010 Bacterial artificial chromosome (BAC) library is an important tool in genomic research. We constructed two libraries from the genomic DNA of grass carp (Ctenopharyngodon idellus) as a crucial part of the grass carp genome project.
Dong, Feng +8 more
core +1 more source
Construction of Gene Targeting Vectors from λKOS Genomic Libraries
BioTechniques, 1999 We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors.
Sigrid Wattler +2 more
doaj +1 more source
Scientific Reports, 2022 The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
doaj +1 more source
Comparing Three Approaches [PDF]
, 2014 Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii ...
Amstislavskiy, Vyacheslav S. +5 more
core +1 more source