Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
core +2 more sources
Exome sequencing from nanogram amounts of starting DNA: comparing three approaches. [PDF]
PLoS ONE, 2014 Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii ...
Vera N Rykalina +5 more
doaj +1 more source
Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]
PLoS ONE, 2010 BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann +4 more
doaj +1 more source
2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]
, 2017 Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn +10 more
core +4 more sources
Using partial genomic fosmid libraries for sequencing complete organellar genomes [PDF]
BioTechniques, 2006 Organellar genome sequences provide numerous phylogenetic markers and yield insight into organellar function and molecular evolution. These genomes are much smaller in size than their nuclear counterparts; thus, their complete sequencing is much less expensive than total nuclear genome sequencing, making broader phylogenetic sampling feasible. However;
McNeal, Joel R. +5 more
openaire +4 more sources
The Human Pregnancy-Specific Glycoprotein Genes are Tightly Linked on the Long Arm of Chromosome 19 and are Coordinately Expressed [PDF]
, 1990 The pregnancy-specific glycoprotein (PSG) genes encode a group of proteins which are found in large amounts in placenta and maternal serum. In situ hybridization analyses of metaphase chromosomes reveal that all the human pregnancy-specific glycoprotein (
Barnett +40 more
core +1 more source
Construction of Gene Targeting Vectors from λKOS Genomic Libraries
BioTechniques, 1999 We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors.
Sigrid Wattler +2 more
doaj +1 more source
Scientific Reports, 2022 The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
doaj +1 more source
Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP. [PDF]
, 2009 Sequence analysis of a 9-kb genomic fragment of the actinobacterium Rhodococcus opacus 1CP led to identification of an open reading frame encoding a novel fusion protein, StyA2B, with a putative function in styrene metabolism via styrene oxide and ...
Berkel, W.J.H., van +5 more
core +4 more sources
Biases in the SMART-DNA library preparation method associated with genomic poly dA/dT sequences.
PLoS ONE, 2017 Avoiding biases in next generation sequencing (NGS) library preparation is crucial for obtaining reliable sequencing data. Recently, a new library preparation method has been introduced which has eliminated the need for the ligation step.
Oriya Vardi +4 more
doaj +1 more source