Results 301 to 310 of about 633,728 (345)
Some of the next articles are maybe not open access.
Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries
2011Large insert genome libraries have been a core resource required to sequence genomes, analyze haplotypes, and aid gene discovery. While next generation sequencing technologies are revolutionizing the field of genomics, traditional genome libraries will still be required for accurate genome assembly.
Mike A, Quail +5 more
openaire +2 more sources
Current Protocols in Molecular Biology, 1987
AbstractGenomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The
openaire +2 more sources
AbstractGenomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The
openaire +2 more sources
Rapid Expression of Functional Genomic Libraries
Journal of Proteome Research, 2006Genomic-scale analysis of protein function is currently limited by the ability to rapidly express the enormous diversity of protein targets in their active form. We describe a method to construct transcriptionally active expression templates (ETs) in parallel using a single PCR step wherein the overlap-extension reaction for addition of transcription ...
Kim A, Woodrow +2 more
openaire +2 more sources
Whole-Genome Sequencing: Automated, Nonindexed Library Preparation
Cold Spring Harbor Protocols, 2016This protocol describes an automated procedure for constructing a nonindexed Illumina DNA library and relies on the use of a CyBi-SELMA automated pipetting machine, the Covaris E210 shearing instrument, and the epMotion 5075. With this method, genomic DNA fragments are produced by sonication, using high-frequency acoustic energy to shear DNA.
Elaine, Mardis, W Richard, McCombie
openaire +3 more sources
OBTAINING GENOMIC DNA FOR CONSTRUCTION OF GENOMIC LIBRARY
Acta Horticulturae, 2010The isolation of pure genomic DNA from the white and red dragon fruits, Hylocereus undatus and Hylocereus polyrhizus (Cactaceae) for the construction of a genomic library is described. These vine cacti originate from northern South America, Central America and Mexico, and are cultivated as new exotic fruit crops in SE Asia.
L.K.P. Daniel, Z.X. Loo, S. Chandran
openaire +1 more source
Genetic engineering: Lassoing genomic libraries
Nature Biomedical Engineering, 2017Long single-stranded DNA probes enable the capture and multiplexed cloning of DNA at the megabase scale.
Nathan B. Lubock, Sriram Kosuri
openaire +1 more source
1998
1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
openaire +1 more source
1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
openaire +1 more source
Fosmid Libraries for Genomic Structural Variation Detection
Current Protocols in Human Genetics, 2007AbstractFosmid libraries have demonstrated their utility for a number of applications. These include filling gaps between BACs and small insert libraries in sequence assemblies, performing hybridization/screening studies to isolate functional elements within the genome (Vergin et al., 1998), and detecting insertions, deletions, and rearrangements in ...
William F, Donahue, Heather M, Ebling
openaire +2 more sources
Plant Genomic Sequencing Using Gene‐Enriched Libraries
ChemInform, 2007AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
openaire +2 more sources
Library Instruction in Genome Informatics
Science & Technology Libraries, 1996The bibliographic instruction program at the Library of the University of California, San Francisco, has added a new class that introduces faculty and students to the use of the Intemet for accessing human genome databases. The class includes an overview of the organization and information content of the major genomic databases such as GenBank, PIR and
openaire +1 more source