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Fosmid Libraries for Genomic Structural Variation Detection
Current Protocols in Human Genetics, 2007AbstractFosmid libraries have demonstrated their utility for a number of applications. These include filling gaps between BACs and small insert libraries in sequence assemblies, performing hybridization/screening studies to isolate functional elements within the genome (Vergin et al., 1998), and detecting insertions, deletions, and rearrangements in ...
Heather Ebling, William F. Donahue
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Construction of an ordered genomic library of Mycoplasma genitalium
Gene, 1994As a first step towards sequencing the chromosome of the suspected human pathogen Mycoplasma genitalium, we attempted to clone its entire genome in a set of ordered cosmids. Cosmid libraries were established by partial digestion of M. genitalium genomic DNA with Sau3AI or EcoRI.
Clyde A. Hutchison +8 more
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Construction of a Pseudomonas aeruginosa Genomic DNA Library
2014Although the completion and annotation of the entire genomic DNA sequence of Pseudomonas aeruginosa PAO1 strain has been carried out, an important number of genes are still of unknown function and many genetic elements involved in various regulatory pathways like small RNA are still unrevealed. One of the strategies to identify gene function or genetic
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Construction of a Genomic Library in Lambda Phage
1994In most respects, preparation of maize genomic libraries in lambda phage is similar to preparing any other genomic library. We follow the procedures for cloning with lambda phage vectors detailed by Sambrook et al. (1989) with the variations described below. We focus here on those aspects that may require special attention when using maize DNA.
Clifford F. Weil, Thomas E. Bureau
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Genomic DNA Libraries, Construction and Applications [PDF]
, 2006 By virtue of the powerful technology developed in molecular biology, it is possible to isolate any DNA fragment in the genome of an organism and, after reverse transcription, any transcribed gene in the form of a complementary DNA. The isolation (cloning) procedure involves the insertion of the DNA fragment into a vector, capable of replication in a ...
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Amplification of Genomic Libraries
Cold Spring Harbor Protocols, 2006David W. Russell, Joseph Sambrook
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[29] Preparation of a genomic cosmid library
1987Publisher Summary This chapter focuses on the methodology used in laboratory for the construction of cosmid libraries using the vector pSV13. The preparation of a successful library depends upon the preparation of high quality high-molecular-weight genomic DNA and appropriate 40 kb partial fragments from this DNA, appropriate preparation of the ...
Robert Fleischmann +2 more
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The construction of genomic library
Cell Biology International Reports, 1990openaire +2 more sources
Construction of a genomic library
2001J. M. Crampton, P. A. Bates, T. Knapp
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