Results 171 to 180 of about 456,399 (317)

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Patient‐Derived 3D Heart‐On‐a‐Chip Model of Dilated Cardiomyopathy With Embedded Bead‐Based Mapping of Tissue Contractility

Advanced Healthcare Materials, EarlyView.
A heart‐on‐a‐chip model of dilated cardiomyopathy is developed from patient‐derived induced pluripotent stem cells. The model recapitulates key disease phenotypes and enables functional assessment through integrated bead‐based tracking and pillar deflection measurements.
Ali Mousavi, Ludovic Mouttet, Shihao Cui, Yasaman Hekmatnia, Mehran Mottahedi, Ida Derish, Naimeh Rafatian, Mark Aurousseau, Gregor Andelfinger, Renzo Cecere, Houman Savoji   +10 more
wiley   +1 more source

A direct-from-serum method for calculating a type 1 diabetes genetic risk score. [PDF]

J Transl Med
Spurrier B, Oram RA, Thomas NJ, Locke JM.   +3 more
europepmc   +1 more source

Genotyping-By-Sequencing for diverse applications in biology

, 2016
Genotyping-by-Sequencing (GBS) is a method used to develop rapid and cost-effective high-density genetic marker data for diverse applications in biology.
Shannon Clarke (3504989), Andrew Griffiths (3440687), Rachael Ashby (15393782), Marty Faville (5072459), John McEwan (45371), Tracey Van Stijn (10380614), Rayna Anderson (3504983), Mingshu Cao (5072465), Rudiger Brauning (47247), Ken Dodds (3501542), Rachel Tan (4605598), Jeanne Jacobs (4091890), Alan McCulloch (517237)   +12 more
core  

Overcoming Photodynamic Therapy Resistance: GSH‐Responsive AIE Photosensitizer‐Hydroxycamptothecin Nanoliposomes for Extrahepatic Cholangiocarcinoma

Advanced Healthcare Materials, EarlyView.
A GSH‐responsive AIE nanoplatform (TSH NPs) with RGD‐mediated tumor targeting co‐delivers a photosensitizer (TPA) and HCPT for extrahepatic cholangiocarcinoma. The system synergistically enhances PDT and chemotherapy by suppressing HIF‐1α/VEGF‐driven hypoxic adaptation, effectively overcoming PDT resistance.
Yong Qu, Tao Peng, Yating Zhang, Juanmei Cao, Birong Wang, Chuxing Chai, Zhiyuan Gao, Yiting Xu, Yifan Jin, Yuqing Wang, Zhuoxia Li, Xu Liu, Tianqi Zhao, Yufan Chen, Weimin Lv, Di Lu, Changzheng Huang, Dan Ding, Jinxiang Zhang, Min Li   +19 more
wiley   +1 more source

Bronchoalveolar lavage fluid <i>EGFR</i> genotyping during bronchoscopic biopsy: concordance with tissue and additional molecular diagnostic yield in lung cancer. [PDF]

Transl Lung Cancer Res
Kim DK, Hur JY, Kim HJ, Kim IA, Lee JE, Chung C, Kang DH, Kim SY, Hong G, Park D, Lee KY.   +10 more
europepmc   +1 more source

Elastomeric 3D‐Printed Microenvironments Enable Nanonewton Force Measurements in Healthy and Diseased Human Pluripotent Stem Cell‐Derived Neuroepithelial Cells

Advanced Materials, EarlyView.
We present elastomeric three‐dimensional (3D) microstructures fabricated via two‐photon polymerization (2PP) and post‐processed through wet etching, for quantifying nanonewton (nN)‐scale forces applied by healthy and diseased neural cells. The mechanically characterized free‐standing beam architectures enable measurement of traction forces of ...
Pieter F. J. van Altena, Lucia Castillo Ransanz, Ruben H. Guis, Giulia Bergamaschi, Nanne J. Paauw, Urs Staufer, Vivi M. Heine, Angelo Accardo   +7 more
wiley   +1 more source

Upscaling Genotyping by Amplicon Sequencing With GBAS-GUI. [PDF]

Mol Ecol Resour
Sonnenberg S, Vijayan T, Rupprecht C, Krenn YP, Gruber M, Dorfer H, Kwikiriza G, Meimberg H, Curto M.   +8 more
europepmc   +1 more source

An Automated Allele-calling System for High-throughput

, 2003
Microsatellite markers are widely used for genetic analysis in biomedical re-search, agriculture, population and evolutionary biology, as well as for forensics and diagnostics.
Microsatellite Genotyping, Pratyaksha Jagad Wirapati   +1 more
core  

Microfluidic Droplet Plate Preserving Single Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Morphology and Function

Advanced Materials Technologies, EarlyView.
We introduce a novel method combining microfluidics, optogenetics, and human induced pluripotent stem cell (iPSC) technology to encapsulate single iPSC‐derived cardiomyocytes (iPSC‐CMs) in an optimal environment for functional characterization. This method enables direct genotype‐phenotype correlation for genetic studies and high‐throughput screening ...
Xiao‐Ting Wang, Rachel P. White, Yashasvi Tharani, Michael A. Finocchiaro, Leona M. Ripple, Maksymilian Prondzynski, Tania Konry, Vassilios J. Bezzerides   +7 more
wiley   +1 more source