Results 171 to 180 of about 3,671 (252)

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

Plant Biotechnology Journal, EarlyView.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee, Corinne Best, Evelyn V. Wachowski, Pal Maliga   +3 more
wiley   +1 more source

Integrative and conjugative elements carrying high-level gentamicin resistance genes in <i>Streptococcus dysgalactiae</i> subsp. <i>equisimilis</i> from horses. [PDF]

Microb Genom
Rochegüe T, Hughes S, Saras E, Gillet B, Drapeau A, Glaser P, Madec JY, Haenni M, Lupo A.   +8 more
europepmc   +1 more source

The effects of far‐UVC irradiation on the presence and concentration of ESKAPEE pathogens on hospital surfaces: Study protocol for a multi‐site, double‐blinded randomized controlled trial in La Paz, Bolivia

Photochemistry and Photobiology, EarlyView.
The protocol of a multi‐site, double‐blinded, clustered randomized controlled trial (cRCT) with a binary outcome of the presence of viable presumptive ESKAPEE pathogens on surfaces in and around hospital sinks is to be implemented in two Bolivian hospitals.
Lindsay B. Saber, Melani Rojas, Darcy M. Anderson, Deverick J. Anderson, Holger Claus, Ryan Cronk, Karl G. Linden, Megan E. J. Lott, Lewis J. Radonovich Jr., Bobby G. Warren, Richard D. Williamson, Richard L. Vincent, Sergio Gutiérrez‐Cortez, Carla Calderón Toledo, Joe Brown   +14 more
wiley   +1 more source

Unveiling the molecular mechanisms of burn injury through integrated single-cell and bulk transcriptomic analysis. [PDF]

PLoS One
Zhu X, Huang N, Pan Y, Le X, Cui S, Li J, Fan Y.   +6 more
europepmc   +1 more source

Ultrasound-stimulated BTO-BSA-gen inhibits implant-associated infections by suppressing bacterial adhesion and potentiating aminoglycosides. [PDF]

J Nanobiotechnology
Ding J, He R, Tao S, Wei XE, Yuan X, Cao L.   +5 more
europepmc   +1 more source

Engineered surface strategies to manage dental implant‐related infections

Periodontology 2000, EarlyView.
Abstract When exposed to the oral environment, dental implants, like natural surfaces, become substrates for microbial adhesion and accumulation, often leading to implant‐related infections—one of the main causes of implant failure. These failures impose significant costs on patients, clinicians, and healthcare systems.
João Gabriel S. Souza, Bruna E. Nagay, Rodrigo Martins, Martinna Bertolini, Jamil A. Shibli, Conrado Aparicio, Magda Feres, Valentim A. R. Barão   +7 more
wiley   +1 more source

Beyond the Standard Antibiogram: Unveiling the Roles of pH and Salinity in Modulating Gentamicin Efficacy Against Escherichia coli in Urinary Tract Infections. [PDF]

Microbiologyopen
Rai A, Saoud MEE, Tabchouche N, Bendif H, Garzoli S, Mouni L.   +5 more
europepmc   +1 more source

Antibiotic‐mediated immune modulation in periodontitis

Periodontology 2000, EarlyView.
Abstract Periodontitis is a chronic inflammatory disease affecting the supporting structures of the teeth. Although initiated by dysbiotic microbial communities, its progression is largely driven by the host's uncontrolled inflammatory response. While antibiotics have conventionally been employed in periodontitis therapy for their antimicrobial ...
Lina J. Suárez, R. M. Arce, Camila Pinheiro Furquim, Cristiane Gonçalves, Alpdogan Kantarci, Magda Feres, Nidia C. Castro dos Santos   +6 more
wiley   +1 more source

Methylobacterium populi Peritonitis Associated with Continuous Ambulatory Peritoneal Dialysis. [PDF]

Am J Trop Med Hyg
Hashimoto T, Uchida H, Shibata H, Hiramatsu K.   +3 more
europepmc   +1 more source

Techniques for subretinal injections in animals

Veterinary Ophthalmology, Volume 28, Issue 2, Page 506-518, March 2025.
Abstract Subretinal injections are not commonly performed during clinical treatment of animals but are frequently used in laboratory animal models to assess therapeutic efficacy and safety of gene and cell therapy products. Veterinary ophthalmologists are often employed to perform the injections in the laboratory animal setting, due to knowledge of ...
Ryan F. Boyd, Simon M. Petersen‐Jones
wiley   +1 more source