Results 151 to 160 of about 3,588 (226)

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

open access: yesNew Phytologist, EarlyView.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng   +4 more
wiley   +1 more source

Structural Elucidation and Engineering of the (S)‐scoulerine 2‐O‐Methyltransferase Enabling Regioselective Epiberberine Biosynthesis in Coptis chinensis

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Protoberberine alkaloids are a characteristic group of natural products in Coptis plants known for their notable pharmacological activities. However, the structural similarity and the substrate promiscuity of their biosynthetic enzymes have left the precise synthetic pathways remain unclarified, posing challenges to regulate product formation.
Jun Song   +14 more
wiley   +1 more source

A Modified Cas9 Scaffold Allows Extension of the Virus‐Induced Gene Editing Technology to the Large Potyvirus Genus

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss   +3 more
wiley   +1 more source

The effects of far‐UVC irradiation on the presence and concentration of ESKAPEE pathogens on hospital surfaces: Study protocol for a multi‐site, double‐blinded randomized controlled trial in La Paz, Bolivia

open access: yesPhotochemistry and Photobiology, EarlyView.
The protocol of a multi‐site, double‐blinded, clustered randomized controlled trial (cRCT) with a binary outcome of the presence of viable presumptive ESKAPEE pathogens on surfaces in and around hospital sinks is to be implemented in two Bolivian hospitals.
Lindsay B. Saber   +14 more
wiley   +1 more source

Plasmid-linked virulence genes and high-level gentamicin resistance in <i>Enterococcus faecalis</i> from persistent endodontic infections. [PDF]

open access: yesJ Conserv Dent Endod
Castillo-Guevara A   +5 more
europepmc   +1 more source

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