Results 321 to 330 of about 24,196 (383)
Some of the next articles are maybe not open access.
Human embryonic stem cell and embryonic germ cell lines
Trends in Biotechnology, 2000Undifferentiated human embryonic stem (ES) cells and embryonic germ (EG) cells can be cultured indefinitely and yet maintain the potential to form many or all of the differentiated cells in the body. Human ES and EG cells provide an exciting new model for understanding the differentiation and function of human tissue, offer new strategies for drug ...
J A, Thomson, J S, Odorico
openaire +2 more sources
Serum-free culture of murine primordial germ cells and embryonic germ cells
Theriogenology, 2003Fetal calf serum (FCS) has usually been used for culture of embryonic stem (ES) cell as a component of the culture medium. However, FCS contains undefined factors, which promote cell proliferation and occasionally stimulate differentiation of ES cells. Recently, a chemically-defined serum replacement, Knockout Serum Replacement (KSR), was developed to ...
Takuro, Horii +3 more
openaire +2 more sources
Human primordial germ cells and embryonic germ cells, and their use in cell therapy
Current Opinion in Biotechnology, 2005Human embryonic germ (hEG) cells derive from the transformation of primordial germ cells (PGCs) under appropriate culture conditions with embryonic fibroblast feeder cells. Although the pluripotent and proliferative capacity of hEG cells is thought to be equivalent to that of human embryonic stem (hES) cells, the difficulties of isolating and ...
Behrouz, Aflatoonian, Harry, Moore
openaire +2 more sources
Derivation of embryonic germ cells and male gametes from embryonic stem cells
Nature, 2003Egg and sperm cells (gametes) of the mouse are derived from a founder population of primordial germ cells that are set aside early in embryogenesis. Primordial germ cells arise from the proximal epiblast, a region of the early mouse embryo that also contributes to the first blood lineages of the embryonic yolk sac. Embryonic stem cells differentiate in
Niels, Geijsen +5 more
openaire +2 more sources
Gene Expression Patterns, 2007
We used cDNA array to compare the relative transcript levels of 96 cell cycle-related genes in mouse primordial germ cells (PGCs), embryonic germ (EG) cells and embryonic stem (ES) cells. Among 38 genes of the G1 phase analysed, Ccnd3 (CyclinD3), Cdkn1c (p57(kip2)), Rb1, and Tceb1l (Skip1-like) were expressed at significantly higher levels in PGCs than
Sorrentino, E +4 more
openaire +4 more sources
We used cDNA array to compare the relative transcript levels of 96 cell cycle-related genes in mouse primordial germ cells (PGCs), embryonic germ (EG) cells and embryonic stem (ES) cells. Among 38 genes of the G1 phase analysed, Ccnd3 (CyclinD3), Cdkn1c (p57(kip2)), Rb1, and Tceb1l (Skip1-like) were expressed at significantly higher levels in PGCs than
Sorrentino, E +4 more
openaire +4 more sources
Isolation and Culture of Embryonic Germ Cells
2003Publisher Summary In mammals, three types of pluripotent stem cells have been identified and isolated into culture; these are embryonic stem (ES) cells, embryonic germ (EG) cells, and embryonal carcinoma (EC) cells. These pluripotent stem cells share two important properties.
Maria P, De Miguel, Peter J, Donovan
openaire +2 more sources
Transgenesis and Nuclear Transfer Using Porcine Embryonic Germ Cells
Cloning and Stem Cells, 2007Embryonic germ (EG) cells are undifferentiated stem cells isolated from cultured primordial germ cells (PGC). Porcine EG cell lines with capacities of both in vitro and in vivo differentiation have been established. Because EG cells can be cultured indefinitely in an undifferentiated state, they may be more suitable for nuclear donor cells in nuclear ...
Kwang Sung, Ahn +5 more
openaire +2 more sources
Isolation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation
Transgenic Research, 2003The isolation of murine embryonic stem (ES) cells has been almost exclusively from the 129 mouse strain. Other mouse strains, such as CBA, have proven refractory to ES cell isolation by conventional means. We previously reported the isolation of 87.5% CBA ES lines by selective ablation of differentiating cells (McWhir et al., 1996). Here, we report the
Ed J, Gallagher +3 more
openaire +2 more sources
Characteristics of human embryonic stem cells, embryonal carcinoma cells and embryonic germ cells
2004A stem cell can replicate itself and produce cells that take on more specialized functions. The breadth of function adopted by the more differentiated daughter cells and their progeny is commonly referred to as the developmental potential or potency of the stem cell. The inner cell mass gives rise to two groups of cells.
Michael J. Shamblott +1 more
openaire +1 more source
Black carp vasa identifies embryonic and gonadal germ cells
Development Genes and Evolution, 2017Identification of molecular markers is an essential step in the study of germ cells. Vasa is an RNA helicase and a well-known germ cell marker that plays a crucial role in germ cell development. Here, we identified the Vasa homolog termed Mpvasa as the first germ cell marker in black carp (Mylopharyngodon piceus).
Ting Xue +9 more
openaire +2 more sources