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Electroformation of giant unilamellar vesicles in saline solution.

Colloids and Surfaces B: Biointerfaces, 2016
Giant unilamellar vesicle (GUV) formation on indium tin oxide (ITO) electrodes in saline solution and from charged lipids has proven to be difficult in the past. Yet the best cell membrane models contain charged lipids and require physiological conditions. We present a way to overcome this problem by using plasma cleaned ITO electrodes.
Qingchuan Li   +4 more
semanticscholar   +5 more sources

Advances in giant unilamellar vesicle preparation techniques and applications.

Advances in Colloid and Interface Science, 2023
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly ...
Karthika S Nair, Harsha Bajaj
semanticscholar   +3 more sources

Protein Reconstitution Inside Giant Unilamellar Vesicles.

Annual Review of Biophysics, 2021
Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light
Thomas Litschel, P. Schwille
semanticscholar   +5 more sources

Giant Unilamellar Vesicle Microarrays for Cell Function Study.

Analytical Chemistry, 2018
Giant unilamellar vesicles (GUVs) are widely used as artificial cell models which contribute to elucidate fundamental questions on origin of life and cell functions.
C. Zhu   +3 more
semanticscholar   +4 more sources

Electroformation of Giant Unilamellar Vesicles: Investigating Vesicle Fusion versus Bulge Merging.

Langmuir, 2016
Partially ordered stacks of phospholipid bilayers on a flat substrate can be obtained by the evaporation of a spread droplet of phospholipid-in-chloroform solution. When exposed to an aqueous buffer, numerous micrometric buds populate the bilayers, grow in size over minutes, and eventually detach, forming the so-called liposomes or vesicles.
Y. Micheletto   +3 more
semanticscholar   +3 more sources

Acoustical deformability of giant unilamellar vesicles

The Journal of the Acoustical Society of America, 2017
An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian, G. Silva, B. Drinkwater
semanticscholar   +2 more sources

Bacterial Outer-Membrane-Mimicking Giant Unilamellar Vesicle Model for Detecting Antimicrobial Permeability.

Langmuir, 2023
The construction of bacterial outer membrane models with native lipids like lipopolysaccharide (LPS) is a barrier to understanding antimicrobial permeability at the membrane interface.
Samir Nandi   +2 more
semanticscholar   +1 more source

Microdomain evolution on giant unilamellar vesicles

Biomechanics and Modeling in Mechanobiology, 2012
A chemo-mechanical model is used to capture the formation and evolution of microdomains on the deforming surface of giant unilamellar vesicles. The model is intended for the regime of vesicle dynamics characterized by a distinct difference in time scales between shape change and species transport.
Anand, Embar, John, Dolbow, Eliot, Fried
openaire   +2 more sources

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