PCR cloning Intermediated Gibson assembly (PIG) for Constructing DNA Repair Templates in CRISPR-Cas9 Based Gene Editing. [PDF]
MicroPubl Biol, 2023 Han D, Churcher S, Nordman JT.
europepmc +1 more source
“Lithium‐Free” Anode Solid‐State Batteries Enabled by 3D Structured Miec Garnet
Advanced Energy Materials, EarlyView.“Lithium‐free” anode solid‐state batteries can maximize energy density by eliminating excess lithium metal but are limited by unstable lithium nucleation and poor lithium reversibility at solid interfaces. A mixed ionic and electronic conducting garnet scaffold promotes uniform lithium deposition within a three‐dimensional porous framework at low ...
George V. Alexander +6 more
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Oral History Interview with Kelly Gibson, May 20, 2005
, 2005 The National Museum of the Pacific War presents an oral interview with Kelly Gibson. Gibson joined the Army Air Corps in September, 1941. He was trained as a radio repairman and serviced radios in airplanes at Turner Field, Georgia.
Gibson, Kelly
core
4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts
Life Science AllianceTo streamline expression construct preparation for screening in diverse expression hosts, we present vectors and protocols using Golden Gate ligation to guide one-step Gibson assembly. Multisubunit protein complexes are central to many cellular processes,
Michael Taschner +3 more
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L‐Cysteine and N‐Acetylcysteine Supplementation Improves Clinical Outcome in a Patient With COXPD10
American Journal of Medical Genetics Part A, EarlyView.ABSTRACT MTO1 is a nuclear gene that encodes a mitochondrial protein essential for modifying mitochondrial transfer RNAs (tRNAs) and stabilizing codon‐anticodon interactions to ensure accurate and efficient mitochondrial protein synthesis and oxidative phosphorylation.
Nishitha R. Pillai +5 more
wiley +1 more source
DNA Cloning (Gibson Assembly, Transformation, Plating and Incubation) v2
, 2023 2023 NUS-Singapore iGEM Team followed this protocol to construct the plasmid of interest or to clone the plasmid of interest.
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Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors
Bio-ProtocolSynthetic trans-acting small interfering RNAs (syn-tasiRNAs) are 21-nucleotide small RNAs designed to induce highly specific and efficient gene silencing in plants.
Adriana Cisneros +3 more
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The use of Gibson Assembly for DNA cloning
, 2022 This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made.
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Gibson Assembly® Protocol (E5510) v2
, 2020 This protocol explains methods for the Gibson Assembly using the Gibson Assembly® Cloning Kit (E5510).
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DNA Cloning (Gibson Assembly, Transformation, Plating and Incubation) v1
, 2023 2023 NUS-Singapore iGEM Team followed this protocol to construct the plasmid of interest or to clone the plasmid of interest.
openaire +1 more source