Results 181 to 190 of about 1,453,330 (269)
Protocol for Gibson Assembly (PrimeStar + In-Fusion) v1
Procedure for cloning using Gibson Assembly using PrimeStar and In-Fusion ...
openaire +1 more source
The sorting nexin Snx21 promotes flotillin‐mediated endocytosis
The FEBS Journal, EarlyView.In this study, we describe a novel function of the previously uncharacterized Snx21 protein as a specific positive regulator of flotillin‐mediated endocytic trafficking. Snx21 is recruited to endosomal membranes via binding to PI(3)P, and it colocalizes with flotillins on the surface of late endosomes.
Tamás Maruzs +9 more
wiley +1 more source
Either of the two tea sets that Elizabeth chooses will do very well, though Nelly prefers the broad gold band to flowered border -- she is anxious to bestow the Sevres China set on Dr. Henry Daingerfield for his devotion to the family during their recent
Lewis, Eleanor Parke Custis, 1779-1852
core
Gibson Assembly of PCR-Produced DNA Fragments
, 2019 In molecular biology, the polymerase chain reaction (PCR) is a tool that allows for the selective amplification of any specific target DNA sequence and is widely used in the construction of recombinant DNA molecules.
Bassler, Brandon A.
core
An efficient method to clone TAL effector genes from Xanthomonas oryzae using Gibson assembly. [PDF]
Mol Plant Pathol, 2019 Li C +5 more
europepmc +1 more source
The FEBS Journal, EarlyView.θ‐CA3 from a marine diatom has an N‐terminal transmembrane helix and two homologous catalytic domains. The enzyme functions as a dimer, with active sites formed near the dimer interface. A bicarbonate ion binds on the symmetry axis of the dimer and forms hydrogen bonds with both monomers, thereby activating θ‐CA3 and tuning CO2 hydration activity ...
Hiroto Negoro +5 more
wiley +1 more source
Letter: Eleanor Parke Custis Lewis, Woodlawn, to Elizabeth Bordley Gibson, Philadelphia, 1823 May 7
Nelly recommends a remedy for Elizabeth's headaches -- she recommends Seidlitz powders and [American] Poplar leaves, which have worked on her own -- woodbine, or Carolina Jessamine, are the same plant, it is in full bloom -- she inquires whether Lorenzo ...
Lewis, Eleanor Parke Custis, 1779-1852
core
Protocol for Gibson Assembly (PrimeStar + In-Fusion) v2
Procedure for cloning using Gibson Assembly using PrimeStar and In-Fusion kits
openaire +1 more source
Fungal gene knockout with Agrobacterium tumefaciens using Gibson assembly v1
, 2019 This method uses Agrobacterium tumefaciens to transfer a piece of TDNA containing a resistance gene (hph for hygromycin in this particular case) flanked by homologous regions of the gene you want to knock out into the fungal spore. The process is broken down into following steps: Assembly of the knockout construct via Gibson assembly Transformation ...
openaire +1 more source
The FEBS Journal, EarlyView.Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo +6 more
wiley +1 more source