Results 221 to 230 of about 3,005 (252)

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong   +5 more
wiley   +1 more source

The "cytokine storm" in infection and sepsis: win the battle but lose the war. [PDF]

open access: yesMil Med Res
Fan JB   +13 more
europepmc   +1 more source

Evolving synchronization of the Gulf Stream and Kuroshio-Oyashio Extension in a changing climate. [PDF]

open access: yesSci Adv
Joh Y   +7 more
europepmc   +1 more source

US-CoastEX: Observation-based probabilistic reanalysis of storm surge and sea level extremes for the United States. [PDF]

open access: yesSci Data
Morim J   +6 more
europepmc   +1 more source

A new murine gram-negative sepsis model with standard care satisfies Sepsis-3 and reproduces clinical pathology. [PDF]

open access: yesIntensive Care Med Exp
Bastow CR   +8 more
europepmc   +1 more source

Oceanic memory of tropical cyclones moderates the Kuroshio current. [PDF]

open access: yesNat Commun
Zhang D   +8 more
europepmc   +1 more source

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