Predicting the substrate specificity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit [PDF]
The volatile compounds that constitute the fruit aroma of ripe tomato (Solanum lycopersicum) are often sequestered in glycosylated form. A homology-based screen was used to identify the gene SlUGT5, which is a member of UDP-glycosyltransferase 72 family ...
Alba+41 more
core +1 more source
Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana
Summary Transient expression in Nicotiana benthamiana offers a powerful and versatile platform for rapid production of complex specialized metabolites. Steroidal glycoalkaloids (SGAs) and steroidal saponins produced by members of the Solanaceae family are known for their diverse structures and activities including antimicrobial, anticancer, antiviral ...
Sachin A. Gharat+6 more
wiley +1 more source
UDP-glucosyltransferase activity of housefly microsomal fraction [PDF]
Housefly UDP-glucosyltransferase activity towards p-nitrophenol was demonstrated in a system in vitro. The activity is localized in the microsomal fraction, requires UDP-glucose, is slightly stimulated by Mg2+ and is activated optimally over a wide range of detergent concentration.
Y Repetto, A Morello
openaire +3 more sources
Mutations in UDP-Glucose:Sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds [PDF]
In higher plants, the most abundant sterol derivatives are steryl glycosides (SGs) and acyl SGs. Arabidopsis (Arabidopsis thaliana) contains two genes, UGT80A2 and UGT80B1, that encode UDP-Glc:sterol glycosyltransferases, enzymes that catalyze the ...
Auer, Manfred+14 more
core +5 more sources
Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa
Summary Saponins constitute a class of characteristic compounds within quinoa grains. In this study, metabolomic analysis identified 20 triterpene saponins, the majority of which were exclusively localized within the pericarp. Furthermore, three crucial glycosyltransferases were identified in quinoa.
Yiming Zhang+14 more
wiley +1 more source
Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling. [PDF]
Information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community.
He, Xue-song+6 more
core
Diverse biological effects of glycosyltransferase genes from Tartary buckwheat [PDF]
Background: Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs)
Chen, Hui+12 more
core +2 more sources
A visual monitoring DNA‐free multi‐gene editing system excised via LoxP::FRT/FLP in poplar
Summary The CRISPR‐Cas9 system has emerged as a potent molecular scalpel for precise genome editing, and profoundly revolutionized plant genetics and breeding, facilitating the development of innovative and improved plant varieties. Typically, the CRISPR‐Cas9 gene‐editing construct is delivered into target organisms via Agrobacterium tumefaciens ...
Ruqian Wu+11 more
wiley +1 more source
Efficient screening methods for glucosyltransferase genes in Lactobacillus strains [PDF]
Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus. Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in α-glucan synthesis from sucrose, was detected in ...
Dijkhuizen, L+3 more
core +2 more sources
Summary Dihydrochalcones are secondary metabolites with manifold dietary and pharmaceutical properties, but with a yet unclear function in plants. The abundance of dihydrochalcones, particularly phloridzin, makes Malus ssp. unique in the plant kingdom. The gene(s)/enzyme(s) for the key reaction in phloridzin biosynthesis, the reduction in the C3‐bridge
Susan Schröpfer+14 more
wiley +1 more source