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Talanta, 2021
In the present study we report the simultaneous determination of glutathione (GSH) and glutathione disulfide (GSSG) by an automated flow method based on the concept of zone fluidics. GSH is quantified selectively in a first run by reaction with o-phthalaldehyde at a mildly basic pH = 8, without interference from GSSG. The latter was also found to react
Apostolia, Tsiasioti +1 more
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In the present study we report the simultaneous determination of glutathione (GSH) and glutathione disulfide (GSSG) by an automated flow method based on the concept of zone fluidics. GSH is quantified selectively in a first run by reaction with o-phthalaldehyde at a mildly basic pH = 8, without interference from GSSG. The latter was also found to react
Apostolia, Tsiasioti +1 more
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Determining Glutathione and Glutathione Disulfide Using the Fluorescence Probe o-Phthalaldehyde
Analytical Biochemistry, 2000Because of the importance of glutathione (GSH) and glutathione disulfide (GSSG) in cellular signal transduction, gene regulation, redox regulation, and biochemical homeostasis, accurate determination of cellular glutathione levels is critical. Several procedures have been developed, but many suffer from overestimating GSSG or from cellular substances ...
A P, Senft, T P, Dalton, H G, Shertzer
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Analytical Biochemistry, 1980
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives has been described.
D J, Reed +5 more
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A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives has been described.
D J, Reed +5 more
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New Photoactivatable Analogues of Glutathione Disulfide
Synthesis, 2006New photoactivatable analogues of glutathione disulfide (GSSG) bearing new benzophenone-like photophores were synthesized by using an improved coupling reaction.
Gilbert Kirsch +2 more
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Coenzyme A Glutathione Disulfide
Circulation Research, 1995Abstract The adrenal gland is involved in the regulation of vascular tone by secretion of vasoactive agents such as catecholamines, neuropeptide Y, or endogenous ouabain. A further potent vasoconstrictor is isolated from bovine adrenal glands and is identified by chromatography, mass spectrometry, UV spectroscopy, and enzymatic
Hartmut Schlüter +10 more
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Clearance of Glutathione Disulfide from Rat Mesenteric Vasculature
Toxicology and Applied Pharmacology, 1994Organs of the digestive tract, including pancreas, small intestine, and colon, have mechanisms to modulate plasma thiol-disulfide balance. Because plasma glutathione disulfide (GSSG) concentration may be elevated from < 1 microM in control rats to over 25 microM during oxidative stress, we examined whether GSSG was cleared from rat mesenteric ...
L J, Dahm, D P, Jones
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Glutathionylation of proteins by glutathione disulfide S-oxide
Biochemical Pharmacology, 2002Aqueous solution of S-nitrosoglutathione (GSNO) underwent spontaneous chemical transformation that generated several glutathione derivatives including glutathione sulfonic acid (GSO3H), glutathione disulfide S-oxide (GS(O)SG), glutathione disulfide S-dioxide, and glutathione disulfide.
Kuo-Ping, Huang, Freesia L, Huang
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Protein disulfide isomerase mediates glutathione depletion-induced cytotoxicity
Biochemical and Biophysical Research Communications, 2016Glutathione depletion is a distinct cause underlying many forms of pathogenesis associated with oxidative stress and cytotoxicity. Earlier studies showed that glutamate-induced glutathione depletion in immortalized murine HT22 hippocampal neuronal cells leads to accumulation of reactive oxygen species (ROS) and ultimately cell death, but the precise ...
Kazushi, Okada +2 more
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Expression of Thermotolerance Following Microinjection of Glutathione Disulfide
Radiation Research, 1988Asynchronous Chinese hamster ovary cells were microinjected with glutathione disulfide (GSSG). Successfully injected cells were scored by coinjecting FITC-dextran with GSSG, followed by fluorescent microscopy. After microinjection, cells were incubated for 2.5 h at 37 degrees C to permit thermotolerance development and then heated at 45 degrees C for ...
C K, Lumpkin +4 more
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Glutathione mixed disulfides and heterogeneity of chicken hemoglobins
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 19921. Adult chicken hemoglobins Hb A and Hb D interact with glutathione disulfide, GSSG. The major hemoglobin, Hb A, forms at least two new components, termed GHb AI and GHb AII, and Hb D forms at least one, GHb DI. 2. At pH 8.0 and 5 degrees C, glutathione disulfide (GSSG) in a molar excess of 50 x took 6 days to complete the reaction, although at pH 8.6
E, Reischl, A L, Dafré
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