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Hypoxia increases plasma glutathione disulfide in rats
Lung, 1989We tested the hypothesis that hypoxia causes cellular oxidative stress by measuring plasma concentrations of glutathione disulfide (GSSG) in rats exposed to acute and subacute hypoxia. In awake, unanesthetized, catheter-implanted rats, exposure to 8% O2 for 10 min caused pulmonary vasoconstriction and increased plasma GSSG. This increase in plasma GSSG
Norbert F. Voelkel +3 more
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Protein-S-S-Glutathione Mixed Disulfides as Models of Unfolded Proteins
Biochemistry, 1994Mixed disulfides between glutathione and the reduced forms of disulfide-bonded proteins were generated and characterized to explore their suitability as models of the unfolded state of newly-synthesized secretory proteins. RNase T1 and alpha-lactalbumin were reduced and converted to mixed disulfide derivatives, named GS-RNase T1 and GS-alpha ...
RUOPPOLO, MARGHERITA, R. B. Freedman
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[70] Determination of glutathione and glutathione disulfide in biological samples
1985Publisher Summary There are a number of procedures, for example, chemical, enzymatic, and chromatographic for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in biological samples. Enzymatic and chromatographic methods for the determination of glutathione in biological samples are described in this chapter. Because GSH readily
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Synthesis and Characterization of the Selenium Analog of Glutathione Disulfide
Analytical Biochemistry, 1993We synthesized the selenium analog of glutathione disulfide by a liquid phase method and named it glutaselenone (i.e., gamma-L-glutamyl-L-selenocysteinylglycine) diselenide. The selenol of selenocysteine was protected by the p-methoxybenzyl group, which was removed by acidolysis with trifluoroacetic acid in the presence of thioanisol. The overall yield
Akira Ohtaka +5 more
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Analytical Biochemistry, 1980
Abstract The total glutathione content of biological samples is conveniently determined with an enzymatic recycling assay based on glutathione reductase ( F. Tietze, 1969 , Anal. Biochem. 27 , 502–522). In the original and several subsequent descriptions of this procedure, glutathione disulfide is selectively determined by assaying samples in which
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Abstract The total glutathione content of biological samples is conveniently determined with an enzymatic recycling assay based on glutathione reductase ( F. Tietze, 1969 , Anal. Biochem. 27 , 502–522). In the original and several subsequent descriptions of this procedure, glutathione disulfide is selectively determined by assaying samples in which
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Expression of Thermotolerance Following Microinjection of Glutathione Disulfide
Radiation Research, 1988Asynchronous Chinese hamster ovary cells were microinjected with glutathione disulfide (GSSG). Successfully injected cells were scored by coinjecting FITC-dextran with GSSG, followed by fluorescent microscopy. After microinjection, cells were incubated for 2.5 h at 37 degrees C to permit thermotolerance development and then heated at 45 degrees C for ...
Gael Sammartino +4 more
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Glutathione Disulfide Variability in Normal Human Blood
Analytical Biochemistry, 1994A prevailing opinion is that glutathione disulfide (GSSG) levels in human blood are very low, but many studies have reported variable results. Therefore, our objective was to determine valid processing conditions for GSSG measurement and apply them to normal human blood samples.
John P. Richie +2 more
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Glutathione mixed disulfides and heterogeneity of chicken hemoglobins
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 19921. Adult chicken hemoglobins Hb A and Hb D interact with glutathione disulfide, GSSG. The major hemoglobin, Hb A, forms at least two new components, termed GHb AI and GHb AII, and Hb D forms at least one, GHb DI. 2. At pH 8.0 and 5 degrees C, glutathione disulfide (GSSG) in a molar excess of 50 x took 6 days to complete the reaction, although at pH 8.6
Alcir Luiz Dafre, Evaldo Reischl
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Determining Glutathione and Glutathione Disulfide Using the Fluorescence Probe o-Phthalaldehyde
Analytical Biochemistry, 2000Because of the importance of glutathione (GSH) and glutathione disulfide (GSSG) in cellular signal transduction, gene regulation, redox regulation, and biochemical homeostasis, accurate determination of cellular glutathione levels is critical. Several procedures have been developed, but many suffer from overestimating GSSG or from cellular substances ...
Timothy P. Dalton +2 more
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ELECTROPHORESIS, 2014
This report describes modifications to a CZE method developed by Serru et al. (Clinical Chemistry 2001, 47, 1321–1324) for the simultaneous analysis of reduced glutathione (GSH) and glutathione disulfide (GSSG). Lowering the pH of the run buffer (75 mmol/L boric acid, 25 mmol/L bis‐Tris) from pH 8.4 to 7.8 markedly improved GSH peak area ...
Jeannine Ory-Ascani +2 more
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This report describes modifications to a CZE method developed by Serru et al. (Clinical Chemistry 2001, 47, 1321–1324) for the simultaneous analysis of reduced glutathione (GSH) and glutathione disulfide (GSSG). Lowering the pH of the run buffer (75 mmol/L boric acid, 25 mmol/L bis‐Tris) from pH 8.4 to 7.8 markedly improved GSH peak area ...
Jeannine Ory-Ascani +2 more
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