Results 91 to 100 of about 958,404 (258)

In Situ Synthesized Glycerol‐Releasing Nanocarriers Unlock Superlubricity in Oil‐Based Lubricants

Macromolecular Materials and Engineering
Superlubricity, a state where friction is virtually non‐existent, mitigates uneconomical energy losses caused by mechanical motion. Glycerol exhibits excellent superlubrication properties, especially in combination with inert coatings.
Sarah S. Lembke, Waraporn Wichaita, Beate Müller, Heloise Therien‐Aubin, Katharina Landfester   +4 more
doaj   +1 more source

Measurement of serum total glycerides and free glycerol by high-performance liquid chromatography1

Journal of Lipid Research, 2006
Serum levels of total glycerides and free glycerol are important indices of lipid metabolism and cardiovascular disease risk. Convenient enzymatic methods of measurement have been available, but they are susceptible to interference.
Hongxia Li, Jun Dong, Wenxiang Chen, Shu Wang, Hanbang Guo, Yong Man, Peisheng Mo, Jianzhai Li   +7 more
doaj   +1 more source

Radiation effects on bacterial cells [PDF]

, 1968
Study reveals the physicochemical and biochemical mechanisms which alter or modify the effects of high-energy radiation on living cells. An in-depth discussion is presented emphasizing the importance of optimizing bacterial treatment with ...
Powers, E. L.
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Enhancing Bioconversion of Crude Glycerol into Butanol and 1,3-Propanediol After Pretreatment by Coupling Fermentation and In Situ Recovery: Effect of Initial pH Control

Fermentation
The sharp rise in the worldwide production of biodiesel has created an excess in the crude glycerol market, so it is essential to develop new added-value alternatives for crude glycerol.
Alejandro Ortega, Alejo Valles, Miguel Capilla, Carmen Gabaldón, Francisco Javier Álvarez-Hornos, Paula Marzal   +5 more
doaj   +1 more source

VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol

Journal of Lipid Research, 1998
We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of
Scott Q. Siler, Richard A. Neese, Elizabeth J. Parks, Marc K. Hellerstein   +3 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across Archaea

Communications Biology
Glycerol is highly abundant in natural ecosystems and serves as both an important carbon source for microorganisms as well as a promising feedstock for industrial applications.
Christian Schmerling, Carsten Schroeder, Xiaoxiao Zhou, Jan Bost, Bianca Waßmer, Sabrina Ninck, Tobias Busche, Lidia Montero, Farnusch Kaschani, Oliver J. Schmitz, Jörn Kalinowski, Markus Kaiser, Sonja-Verena Albers, Christopher Bräsen, Bettina Siebers   +14 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source