Results 71 to 80 of about 557,910 (260)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across Archaea

Communications Biology
Glycerol is highly abundant in natural ecosystems and serves as both an important carbon source for microorganisms as well as a promising feedstock for industrial applications.
Christian Schmerling, Carsten Schroeder, Xiaoxiao Zhou, Jan Bost, Bianca Waßmer, Sabrina Ninck, Tobias Busche, Lidia Montero, Farnusch Kaschani, Oliver J. Schmitz, Jörn Kalinowski, Markus Kaiser, Sonja-Verena Albers, Christopher Bräsen, Bettina Siebers   +14 more
doaj   +1 more source

Penggunaan H-Zeolit dan Tawas dalam Pemurnian Crude Glycerol dengan Proses Adsorpsi dan Koagulasi

Jurnal Kimia Valensi, 2017
Production of biodiesel from used cooking oil byproducts such as crude glycerol with low purity. The crude glycerol containing compounds impurities such as free fatty acids, alcohol, soap, catalyst and water. Compound adsorption of impurities can be done
Isalmi Aziz, Nur Hijjah Bayani Fadhilah, Hendrawati Hendrawati   +2 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Selective breeding of Saccharomyces cerevisiae to increase glycerol levels in wine

OENO One, 1999
Glycerol in wines is at levels of 1 to 10 g/l and improves their body. To breed wine yeast for increased glycerol production, we crossed yeast isolated from natural fermentations with a commercial strain.
Bernard A. Prior, Clelia Baccari, Robert K. Mortimer   +2 more
doaj   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol

Journal of Lipid Research, 1998
We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of
Scott Q. Siler, Richard A. Neese, Elizabeth J. Parks, Marc K. Hellerstein   +3 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

KUALITAS SPERMATOZOA SWIM UP KAMBING PERANAKAN ETAWAH HASIL PEMBEKUAN MENGGUNAKAN METODE VITRIFIKASI DENGAN PERSENTASE GLISEROL YANG BERBEDA

Jurnal Ternak Tropika, 2016
The objective of this research was to examine the effect of glycerol addition in different percentage to quality of swim up spermatozoa from Etawah Grade buck that frozen with vitrification method. This research was carried out at Frozen Semen Laboratory,
Saka Wahyu Hikamawan, Gatot Cipatadi, Sri Wahjuningsih   +2 more
doaj   +1 more source