Results 141 to 150 of about 2,890 (187)

Fluorogenic substrates of glycogen debranching enzyme for assaying debranching activity

Analytical Biochemistry, 2005
Glycogen debranching enzyme (GDE) degrades glycogen in concert with glycogen phosphorylase. GDE has two distinct active sites for maltooligosaccharide transferase and amylo-1,6-glucosidase activities. Phosphorylase limit dextrin from glycogen is debranched by cooperation of the two activities.
Yumiko, Watanabe, Yasushi, Makino, Kaoru, Omichi   +2 more
openaire   +2 more sources

Binding of glycogen, oligosaccharides, and glucose to glycogen debranching enzyme

Biochemistry, 1988
The binding of glucose and a series of oligosaccharides to glycogen debranching enzyme was determined by the ability of the saccharides to decrease the rate of reaction of sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoate) (DTNB). At pH 7.2, the strength of binding increases with chain length from glucose to maltotriose to maltopentaose but not to ...
J, Takrama, N B, Madsen
openaire   +2 more sources

Reassessment of the catalytic mechanism of glycogen debranching enzyme

Biochemistry, 1991
The amylo-1,6-glucosidase catalytic activity of glycogen debranching enzyme allows it to hydrolyze alpha-D-glucosyl fluoride, in the absence or presence of glycogen or oligosaccharides, releasing equal amounts of fluoride and glucose at rates comparable to those seen with the natural substrates. 2-Deoxy-2-fluoro-alpha-D-glucosyl fluoride is found to be
W, Liu, N B, Madsen, C, Braun, S G, Withers   +3 more
openaire   +2 more sources

Preparation and properties of the glycogen-debranching enzyme from rabbit liver

Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Abstract The glycogen-debranching enzyme, oligo-α-1,4-glucan: α-1,4-glucan-4-glycosyl-transferase-amylo-1,6-glucosidase has been purified about 500-fold from rabbit liver. The enzyme preparation has been shown to have both transferase and glucosidase activities.
R B, Gordon, D H, Brown, B I, Brown
openaire   +2 more sources

Effects of oligosaccharide binding on glycogen debranching enzyme activity and conformation

Biochemistry, 1995
Glycogen debranching enzyme contains two catalytic activities (4-alpha-glucanotransferase and amylo-1,6-glucosidase) on its single polypeptide chain, and they are affected differently by the binding of oligosaccharides. Glucose, maltose, and maltotriose are competitive inhibitors of the amylo-1,6-glucosidase activity measured by the hydrolysis of alpha-
W, Liu, N B, Madsen, B, Fan, K A, Zucker, R H, Glew, D E, Fry   +5 more
openaire   +2 more sources

Donor Substrate Specificity of 4- -Glucanotransferase of Porcine Liver Glycogen Debranching Enzyme and Complementary Action to Glycogen Phosphorylase on Debranching

Journal of Biochemistry, 2007
Glycogen debranching enzyme (GDE) has both 4-alpha-glucanotransferase and amylo-alpha-1,6-glucosidase activities. Here, we examined 4-alpha-glucanotransferase action of porcine liver GDE on four 6(4)-O-alpha-maltooligosyl-pyridylamino(PA)-maltooctaoses, in the presence or absence of an acceptor, maltohexaose.
Yumiko, Watanabe, Yasushi, Makino, Kaoru, Omichi   +2 more
openaire   +2 more sources

New approach to prepare fluorogenic branched dextrins for assaying glycogen debranching enzyme

Glycoconjugate Journal, 2020
Glycogen debranching enzyme (GDE), together with glycogen phosphorylase (GP), is responsible for the complete degradation of glycogen. GDE has distinct catalytic sites for 4-α-glucanotransferase and amylo-α-1,6-glucosidase. For the GDE sensitive assay, we previously developed the GP limit fluorogenic branched dextrin Glcα1-4Glcα1-4Glcα1-4Glcα1-4(Glcα1 ...
Miyu Sakaguchi, Yasushi Makino, Hiroshi Matsubara   +2 more
openaire   +2 more sources

Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum

Acta Physiologica Scandinavica, 2004
AbstractAims:  Gel electrophoresis revealed a band of molecular weight ∼160 000 Da associated with the skeletal muscle sarcoplasmic reticulum (SR) vesicle preparations. This investigation sought to examine glycogen debranching enzyme associated with skeletal muscle SR.Methods:  Sarcoplasmic reticulum samples were also taken from muscle whose glycogen ...
S J, Lees, Y-T, Chen, J H, Williams
openaire   +2 more sources

Immunoblot analyses of glycogen debranching enzyme in different subtypes of glycogen storage disease type III

The Journal of Pediatrics, 1990
To determine the tissue distribution of glycogen debranching enzyme, we used immunoblot analysis with a polyclonal antibody prepared against purified porcine muscle debranching enzyme. Debranching enzyme was identified in porcine brain, kidney, cardiac muscle, skeletal muscle, liver, and spleen; and in human liver, skeletal muscle, lymphocytes ...
J H, Ding, T, de Barsy, B I, Brown, R A, Coleman, Y T, Chen   +4 more
openaire   +2 more sources

Glycogen debranching enzyme deficiency: Long‐term study of serum enzyme activities and clinical features

Journal of Inherited Metabolic Disease, 1992
SummaryIn glycogen storage disease type III (glycogen debranching enzyme (DE) deficiency), the activities of serum alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase may be strikingly elevated during childhood but are low during adult life.
R A, Coleman, H S, Winter, B, Wolf, Y T, Chen   +3 more
openaire   +2 more sources