Results 161 to 170 of about 1,170 (199)

Solid-Phase Oligosaccharide and Glycopeptide Synthesis Using Glycosynthases

The Journal of Organic Chemistry, 2002
Enzymatic approaches for the preparation of oligosaccharides are interesting alternatives to traditional chemical synthesis, the main advantage being the regio- and stereoselectivity offered without the need for protecting groups. The use of solid-phase techniques offers easy workup procedures and the prospect of automatability.
Jakob F, Tolborg, Lars, Petersen, Knud J, Jensen, Christoph, Mayer, David L, Jakeman, R Antony J, Warren, Stephen G, Withers   +6 more
openaire   +2 more sources

A novel thermophilic Glycosynthase that effects branching glycosylation

Bioorganic & Medicinal Chemistry Letters, 2000
A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported.
Trincone A, Perugino G, Rossi M, Moracci M   +3 more
openaire   +4 more sources

Unexpected regioselectivity of Humicola insolens Cel7B glycosynthase mutants

Carbohydrate Research, 2007
Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl N(I)-acetyl-2(II)-azido-beta-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a beta-(1-->4) linkage between the two disaccharides, while single mutant Cel7B E197A and
Blanchard, S., Armand, S., Couthino, P., Patkar, S., Vind, J., Samain, E., Driguez, H., Cottaz, S.   +7 more
openaire   +2 more sources

Glycosidases and Glycosynthases

2014
Chemoenzymatic synthesis of glycans as an alternative to classical chemical synthesis has great interest for the exquisite sterospecificity and improved processivity and regioselectivity of the biological catalysts and for the possibility of using reagents less toxic for the environment.
Beatrice CobucciPonzano, Marco Moracci
openaire   +3 more sources

Activity of Hyperthermophilic Glycosynthases Is Significantly Enhanced at Acidic pH

Biochemistry, 2003
We have previously shown that the hyperthermophilic glycosynthase from Sulfolobus solfataricus (Ssbeta-glyE387G) can promote the synthesis of branched oligosaccharides from activated beta-glycosides, at pH 6.5, in the presence of 2 M sodium formate as an external nucleophile.
Perugino G, Trincone A, Giordano A, van der Oost J, Kaper T, Rossi M, Moracci M   +6 more
openaire   +6 more sources

Highly Productive Autocondensation and Transglycosylation Reactions with Sulfolobus solfataricus Glycosynthase

ChemBioChem, 2005
AbstractTransglycosylation reactions (autocondensation of the substrate or transfer of the glycon donor moiety to different acceptors) with the hyperthermophilic glycosynthase from Sulfolobus solfataricus acting in dilute sodium formate buffer at pH 4.0 are reported; the use of 4‐nitrophenyl β‐glucopyranoside as both donor and acceptor in the self ...
A Trincone, A Giordano, G Perugino, M Rossi, M Moracci   +4 more
openaire   +4 more sources

Exploitation of β-glycosyl azides for the preparation of α-glycosynthases

Biocatalysis and Biotransformation, 2012
An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β-glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue.
Cobucci Ponzano B., Zorzetti C., STRAZZULLI, ANDREA, BEDINI, EMILIANO, CORSARO, MARIA MICHELA, Sulzenbacher G., Rossi M., MORACCI, Marco   +7 more
openaire   +6 more sources

Glycosynthase-Assisted Synthesis of Some Glycosylated scyllo-Inositols

Australian Journal of Chemistry, 2006
Three derivatives of scyllo-inositol, a 4-nitrophenyl ether, a benzyl ether, and an ester of 4-toluenesulfonic acid, have been treated with α-d-galactopyranosyl fluoride and a glycosynthase to give the corresponding mono-glycosylated inositols. In one instance, a similar treatment with α-d-glucopyranosyl fluoride gave a mono- and a di-glycosylated ...
Adrian Scaffidi, Robert V. Stick
openaire   +1 more source

ChemInform Abstract: Glycosynthases: Mutant Glycosidases for Oligosaccharide Synthesis.

ChemInform, 1998
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
L. F. MACKENZIE, Q. WANG, R. A. J. WARREN, S. G. WITHERS   +3 more
openaire   +1 more source

The crystal structure of an inverting glycoside hydrolase family 9 exo-β-D-glucosaminidase and the design of glycosynthase.

Biochemical Journal, 2016
Y. Honda, Sachiko Arai, Kentaro Suzuki, M. Kitaoka, S. Fushinobu   +4 more
semanticscholar   +1 more source