Results 1 to 10 of about 5,064 (210)

Comprehensive study on transglycosylation of CGTase from various sources [PDF]

Heliyon, 2021
Transglycosylation is the in-vivo or in-vitro process of transferring glycosyl groups from a donor to an acceptor, which is usually performed by enzymatic reactions because of their simplicity, low steric hindrance, high region-specificity, low ...
Babak Rasti, Chin Hui Lim, Mansoor Abdul Hamid   +2 more
exaly   +6 more sources

Improvement of the Transglycosylation Efficiency of a Lacto-N-Biosidase from Bifidobacterium bifidum by Protein Engineering

Applied Sciences, 2021
The lacto-N-biosidase LnbB from Bifidobacterium bifidum JCM 1254 was engineered to improve its negligible transglycosylation efficiency with the purpose of enzymatically synthesizing lacto-N-tetraose (LNT; Gal-β1,3-GlcNAc-β1,3-Gal-β1,4-Glc) in one ...
Marlene Vuillemin, Jesper Holck, Martin Matwiejuk, Eduardo S. Moreno Prieto, Jan Muschiol, Dora Molnar-Gabor, Anne S. Meyer, Birgitte Zeuner   +7 more
doaj   +2 more sources

Transglycosylation: A mechanism for RNA modification (and editing?) [PDF]

Bioorganic Chemistry, 2005
The vast majority of the ca. 100 chemically distinct modified nucleosides in RNA appear to arise via the chemical transformation of a genetically encoded nucleoside. Two notable exceptions are queuosine and pseudouridine, which are incorporated into tRNA via transglycosylation.
George Garcia
exaly   +3 more sources

Engineering the GH1 β-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose. [PDF]

PLoS ONE, 2017
The activity of the GH1 β-glucosidase from Humicola insolens (Bglhi) against p-nitrophenyl-β-D-glucopyranoside (pNP-Glc) and cellobiose is enhanced 2-fold by glucose and/or xylose.
Luana Parras Meleiro, José Carlos Santos Salgado, Raquel Fonseca Maldonado, Sibeli Carli, Luiz Alberto Beraldo Moraes, Richard John Ward, João Atílio Jorge, Rosa Prazeres Melo Furriel   +7 more
doaj   +1 more source

Manipulation of an α-glucosidase in the industrial glucoamylase-producing Aspergillus niger strain O1 to decrease non-fermentable sugars production and increase glucoamylase activity

Frontiers in Microbiology, 2022
Dextrose equivalent of glucose from starch hydrolysis is a critical index for starch-hydrolysis industry. Improving glucose yield and decreasing the non]-fermentable sugars which caused by transglycosylation activity of the enzymes during the starch ...
Wenzhu Guo, Wenzhu Guo, Dandan Liu, Dandan Liu, Dandan Liu, Jingen Li, Jingen Li, Wenliang Sun, Wenliang Sun, Tao Sun, Tao Sun, Xingji Wang, Kefen Wang, Qian Liu, Qian Liu, Chaoguang Tian, Chaoguang Tian   +16 more
doaj   +1 more source

The characterisation of an alkali-stable maltogenic amylase from Bacillus lehensis G1 and improved malto-oligosaccharide production by hydrolysis suppression. [PDF]

PLoS ONE, 2014
A maltogenic amylase (MAG1) from alkaliphilic Bacillus lehensis G1 was cloned, expressed in Escherichia coli, purified and characterised for its hydrolysis and transglycosylation properties. The enzyme exhibited high stability at pH values from 7.0 to 10.
Nor Hasmaliana Abdul Manas, Samson Pachelles, Nor Muhammad Mahadi, Rosli Md Illias   +3 more
doaj   +1 more source

Structural Insight into a Yeast Maltase—The BaAG2 from Blastobotrys adeninivorans with Transglycosylating Activity

Journal of Fungi, 2021
An early-diverged yeast, Blastobotrys (Arxula) adeninivorans (Ba), has biotechnological potential due to nutritional versatility, temperature tolerance, and production of technologically applicable enzymes. We have biochemically characterized from the Ba
Karin Ernits, Christian Kjeldsen, Karina Persson, Eliis Grigor, Tiina Alamäe, Triinu Visnapuu   +5 more
doaj   +1 more source

Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification. [PDF]

PLoS ONE, 2018
The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties of antibodies. By remodeling N-glycan to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled or ...
Mitsuhiro Iwamoto, Yukiko Sekiguchi, Kensuke Nakamura, Yoshirou Kawaguchi, Takeshi Honda, Jun Hasegawa   +5 more
doaj   +1 more source

Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities

Molecules, 2023
Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN.
Reeba Thomas, Tamo Fukamizo, Wipa Suginta   +2 more
doaj   +1 more source

Enzymatic transglycosylation for glycoconjugate synthesis [PDF]

Current Opinion in Chemical Biology, 2009
Remarkable advances have been made in recent years in exploiting the transglycosylation activity of glycosidases and glycosynthase mutants for oligosaccharide and glycoconjugate synthesis. New glycosynthases were generated from retaining glycosidases, inverting glycosidases, and those that proceed in a mechanism of substrate-assisted catalysis ...
Lai-Xi, Wang, Wei, Huang
openaire   +2 more sources