Results 41 to 50 of about 5,064 (210)

Modulation of the Activity and Regioselectivity of a Glycosidase: Development of a Convenient Tool for the Synthesis of Specific Disaccharides

Molecules, 2021
The synthesis of disaccharides, particularly those containing hexofuranoside rings, requires a large number of steps by classical chemical means. The use of glycosidases can be an alternative to limit the number of steps, as they catalyze the formation ...
Yari Cabezas-Pérusse, Franck Daligault, Vincent Ferrières, Olivier Tasseau, Sylvain Tranchimand   +4 more
doaj   +1 more source

TLC analysis of FOS from transglycosylation reactions.

, 2014
The transglycosylation reactions were catalyzed by native and recombinant BfrAs incubated with 33% sucrose at optimal reaction condition for 5 h. The FOS were separated by Bio-Gel P2 column.
Dongxue Wang (621725), Zhongwu Guo (673969), Lili Lu (408133), Jiawei Liu (553742), Min Xiao (320004), Lan Jin (464006), Li Xu (108702), Deyong Song (403402)   +7 more
core   +1 more source

Increasing the transglycosylation activity of alpha-galactosidase from Bifidobacterium adolescentis DSM 20083 by site-directed mutagenesis

, 2006
The ¿-galactosidase (AGA) from Bifidobacterium adolescentis DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme-substrate
Schipperus, R., Vincken, J.P., van den Broek, L.A.M., Voragen, A.G.J., Hinz, S.W.A., Doeswijk-Voragen, C.H.L.   +5 more
core   +2 more sources

The optimisation of the transglycosylation reaction.

, 2014
The effects of (A) M3 concentration, (B) enzyme concentration, (C) time and (D) temperature on the proportion of hydrolysis or transglycosylation are shown.
Samson Pachelles (629700), Nor Hasmaliana Abdul Manas (629699), Rosli Md. Illias (601938), Nor Muhammad Mahadi (355153)   +3 more
core   +1 more source

Transglycosylation activity of Gt-Mamy.

, 2013
(a) Transglycosylation of hydrolytic products of G4, G5 and acarbose onto G1/G2 (Lanes 1,2,3,4,5,6,7,8,9,10,11 indicate standards, Ac, G1+G5+E, G1+G4+E, G1+Ac+E, G2+G5+E, G2+G4+E, G2+Ac+E, G1+G4+EΔ, G1+G5+EΔ, G1+Ac+EΔ, respectively); (b ...
Deepika Mehta (460758), Tulasi Satyanarayana (278390)   +1 more
core   +1 more source

A Single Hydrogen Bond Controls the Selectivity of Transglycosylation vs Hydrolysis in Family 13 Glycoside Hydrolases

, 2022
International audienceConverting glycoside hydrolases (GHs) from hydrolytic to synthetic enzymes via transglycosylation is a long-standing goal for the biosynthesis of complex carbohydrates.
Xia, Wei, Guo, Zhiyong, Sheng Chen, Wei Xia, Lingqia Su, Chen, Sheng, Rovira, Carme, Zhiyong Guo, Carme Rovira, André, Isabelle, Wu, Jing, Jing Wu, Lei Wang, Wang, Binju, Binju Wang, Wang, Lei, Su, Lingqia, Isabelle André   +17 more
core   +1 more source

Characterization of a Novel Ginsenoside MT1 Produced by an Enzymatic Transrhamnosylation of Protopanaxatriol-Type Ginsenosides Re

Biomolecules, 2020
Background: Ginsenosides, triterpene saponins of Panax species, are considered the main active ingredients responsible for various pharmacological activities.
Byeong-Min Jeon, Jong-In Baek, Min-Sung Kim, Sun-Chang Kim, Chang-hao Cui   +4 more
doaj   +1 more source

Enzymatic preparation of oligosaccharides by transglycosylation: A comparative study of glucosidases [PDF]

, 2015
Five glucosidases were studied with respect to their ability to catalyse the transglycosylation of maltose or cellobiose. Experiments were carried out at high substrate concentrations to increase the ratio between transglycosylation and hydrolysis.
Adlercreutz, Patrick, Adlercreutz, Patrick,, Mangas, Juan, Mangas-Sánchez, Juan   +3 more
core   +1 more source

Intermolecular Transglycosylation of Cyclodextrin Glycosyltansferase

Journal of the Japanese Society of Starch Science, 1979
The acceptor specificity in the intermolecular transglycosylation of cyclodextrin glycosyltransferase (CGT-ase) was investigated using 18 kinds of monosaccharides and their derivatives. It is concluded on the basis of the experimental results that the requirement for an acceptor of the intermolecular transfer reaction catalyzed by CGT-ase is the ...
KITAHATA, Sumio, OKADA, Shigetaka
openaire   +2 more sources

Analysis of Carbohydrates and Glycoconjugates by Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2023–2024

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The use of MALDI mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this comprehensive review is the twelfth update of the original article published in 1999 and brings coverage of the literature to the end of 2024.
David J. Harvey
wiley   +1 more source