Results 51 to 60 of about 4,794 (208)

A Single Hydrogen Bond Controls the Selectivity of Transglycosylation vs Hydrolysis in Family 13 Glycoside Hydrolases

, 2022
International audienceConverting glycoside hydrolases (GHs) from hydrolytic to synthetic enzymes via transglycosylation is a long-standing goal for the biosynthesis of complex carbohydrates.
Xia, Wei, Guo, Zhiyong, Sheng Chen, Wei Xia, Lingqia Su, Chen, Sheng, Rovira, Carme, Zhiyong Guo, Carme Rovira, André, Isabelle, Wu, Jing, Jing Wu, Lei Wang, Wang, Binju, Binju Wang, Wang, Lei, Su, Lingqia, Isabelle André   +17 more
core   +1 more source

Intermolecular Transglycosylation of Cyclodextrin Glycosyltansferase

Journal of the Japanese Society of Starch Science, 1979
The acceptor specificity in the intermolecular transglycosylation of cyclodextrin glycosyltransferase (CGT-ase) was investigated using 18 kinds of monosaccharides and their derivatives. It is concluded on the basis of the experimental results that the requirement for an acceptor of the intermolecular transfer reaction catalyzed by CGT-ase is the ...
KITAHATA, Sumio, OKADA, Shigetaka
openaire   +2 more sources

Safety evaluation of the food enzyme dextransucrase from the genetically modified Bacillus subtilis strain DP‐Eyp97

EFSA Journal, Volume 24, Issue 6, June 2026.
Abstract The food enzyme dextransucrase (sucrose:1,6‐α‐d‐glucan 6‐α‐d‐glucosyltransferase; EC 2.4.1.5) is produced with the genetically modified Bacillus subtilis strain DP‐Eyp97 by Genencor International B.V. The production strain met the requirements for the qualified presumption of safety. The food enzyme was considered free from viable cells of the
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Henk Van Loveren, Laurence Vernis, Cristina Andreoli, Andrea Terron, Cristina Fernández‐Fraguas, Daniele Cavanna, Simone Lunardi, Yi Liu   +18 more
wiley   +1 more source

Shaping the Infant Microbiome With Non-digestible Carbohydrates

Frontiers in Microbiology, 2019
Natural polysaccharides with health benefits are characterized by a large structural diversity and differ in building blocks, linkages, and lengths. They contribute to human health by functioning as anti-adhesives preventing pathogen adhesion, stimulate ...
Stella Verkhnyatskaya, Michela Ferrari, Paul de Vos, Marthe T. C. Walvoort   +3 more
doaj   +1 more source

N-Ribosyltransferase From Archaeoglobus veneficus: A Novel Halotolerant and Thermostable Biocatalyst for the Synthesis of Purine Ribonucleoside Analogs

Frontiers in Bioengineering and Biotechnology, 2020
Nucleoside-2′-deoxyribosyl-transferases (NDTs) catalyze a transglycosylation reaction consisting of the exchange of the 2′-deoxyribose moiety between a purine and/or pyrimidine nucleoside and a purine and/or pyrimidine base.
Javier Acosta, Jon Del Arco, Victor Pisabarro, Federico Gago, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +5 more
doaj   +1 more source

Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity

, 2008
A broad specificity beta-D-glycosidase, designated G1, was purified to homogeneity from the viscera of the China white jade snail (Achatina fulica). The enzyme was a monomeric protein with molecular weight of 115 kDa.
Zhou, Kun, Hu, Ying, Yang, Shengli, 杨凌, Ge, Guangbo, Yang, Ling, Luan, Hongwei   +6 more
core   +1 more source

Development of a liquid chromatography/mass spectrometry assay for the bacterial transglycosylation reaction through measurement of Lipid II [PDF]

, 2015
Transglycosylation is the second to last step in the production of bacterial peptidoglycan. It is catalyzed by a transglycosylation site in class A penicillin-binding proteins (PBPs) or monofunctional glycosyl transferases.
Wyseure, Tine, Breukink, Eefjan, Membrane Biochemistry and Biophysics, Tine Wyseure, Adams, Erwin, Bart Blanchaert, Paul Declerck, Eefjan Breukink, Declerck, Paul, Sub Membrane Biochemistry & Biophysics, Blanchaert, Bart, Van Schepdael, Ann, Erwin Adams, Ann Van Schepdael   +13 more
core   +2 more sources

A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis

Molecules, 2020
An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.
Mpho S. Mafa, Heinrich W. Dirr, Samkelo Malgas, Rui W. M. Krause, Konanani Rashamuse, Brett I. Pletschke   +5 more
doaj   +1 more source

A BROAD-SPECIFICITY Β-GLUCOSIDASE FROM A WILD TYPE OF YEAST ISOLATE AND ITS POTENTIAL USE IN FOOD INDUSTRY [PDF]

Journal of Food and Dairy Sciences, 2007
A novel intracellular β-glucosidase was isolated from a wild type of yeast Trichosporon sp. Production of enzyme was stimulated with highest level of production in medium containing 1 % lactose as a sole carbon source at pH 7 and 30 oC for 48 hr.
M. Salem, M. Korish
doaj   +1 more source

Alteration of Substrate Specificity and Transglucosylation Activity of GH13_31 α-Glucosidase from Bacillus sp. AHU2216 through Site-Directed Mutagenesis of Asn258 on β→α Loop 5

Molecules, 2023
α-Glucosidase catalyzes the hydrolysis of α-d-glucosides and transglucosylation. Bacillus sp. AHU2216 α-glucosidase (BspAG13_31A), belonging to the glycoside hydrolase family 13 subfamily 31, specifically cleaves α-(1→4)-glucosidic linkages and shows ...
Waraporn Auiewiriyanukul, Wataru Saburi, Tomoya Ota, Jian Yu, Koji Kato, Min Yao, Haruhide Mori   +6 more
doaj   +1 more source