Results 31 to 40 of about 4,794 (208)

Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

AMB Express, 2021
The glycosidases within GH5-23 cleave the glycosidic bond of β-glucosylated or rutinosylated flavonoids. Moreover, by virtue of their transglycosylation activity, glycoconjugates with glucosyl and rutinosyl moieties are accessible.
Michael Kotik, Hana Javůrková, Katerina Brodsky, Helena Pelantová   +3 more
doaj   +1 more source

Transglycosylation to Ginseng Saponins by Cyclomaltodextrin Glucanotransferases

Bioscience, Biotechnology, and Biochemistry, 2001
Ten new alpha-glucosylginsenosides were found to be synthesized from dextrin and four ginsenosides, -Rb1, -Rc, -Re, and -Rg1, by the successive actions of B. stearothermophilus cyclomaltodextrin glucanotransferase and Rhizopus glucoamylase. Seven of them were isolated in the pure state by extraction with n-butanol saturated with water, silica gel ...
KIM, Young-Hoi, LEE, Young-Gu, CHOI, Kang-Ju, UCHIDA, Kei, SUZUKI, Yukio   +4 more
openaire   +2 more sources

Introducing transglycosylation activity in a liquefying α‐amylase [PDF]

FEBS Letters, 1999
By mutating Ala‐289 by Phe or Tyr in the Bacillus stearothermophilus α‐amylase, we induced this enzyme to perform alcoholytic reactions, a function not present in the wild‐type enzyme. This residue was selected from homology analysis with neopullulanase, where the residue has been implicated in the control of transglycosylation [Kuriki et al. (1996) J.
Saab-Rincón, Gloria, del-Rı́o, Gabriel, Santamarı́a, Rosa I, López-Munguı́a, Agustı́n, Soberón, Xavier   +4 more
openaire   +2 more sources

Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase

Molecules, 2023
The enzymatic transglycosylation of steviol glycosides can improve the edulcorant quality of steviol glycosides. Cyclodextrin glucanotransferase (CGTase) is one of the most popular glucanotransferases applied in this reaction.
Ruiqin Zhang, Ruiqi Tang, Jiahua Bi, Shanshan Shen, Qin Wu, Qihe Chen, Yanjun Li   +6 more
doaj   +1 more source

Alkyltransferase Ribozyme for Site‐Specific N4‐Cytidine Alkylation

Angewandte Chemie, Volume 138, Issue 20, 11 May 2026.
CSAR is the first ribozyme that catalyzes direct alkylation of an exocyclic amino group of an RNA nucleobase. The ribozyme was isolated as a self‐labeling catalyst from an in vitro selection library and engineered into a trans‐active ribozyme that catalyzes the transfer of a benzyl group from an O6‐modifed guanine to the N4 amino group of a specific ...
Evgeniia Dorinova, Manisha B. Walunj, Claudia Höbartner   +2 more
wiley   +2 more sources

α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2

Czech Journal of Food Sciences, 2005
In this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (α-galactosidase, α-glucosidase, β-glucosidase, α-mannosidase and β-glucuronidase).
Eva Benešová, Michaela Marková, Blanka Králová   +2 more
doaj   +1 more source

Crystal structure of endo-β-N-acetylglucosaminidase HSα. [PDF]

Acta Crystallogr F Struct Biol Commun
Structural analysis of the GH85 enzyme endo‐β‐N‐acetylglucosaminidase HSα reveals a novel domain that is potentially involved in complex N‐glycan recognition.The crystal structure of endo‐β‐N‐acetylglucosaminidase HSα (Endo HSα) was determined at 1.8 Å resolution, revealing that the enzyme is composed of five distinct domains.
Kurauchi I, Okura K, Hosokawa C, Ito K, Miyahara I.   +4 more
europepmc   +2 more sources

Heterologous Expression of a Thermostable α-Glucosidase from Geobacillus sp. Strain HTA-462 by Escherichia coli and Its Potential Application for Isomaltose–Oligosaccharide Synthesis

Molecules, 2019
Isomaltose–oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using α-glucosidase. In the present study, the α-glucosidase (GSJ) from Geobacillus sp.
Fan Zhang, Weiyang Wang, Fatoumata Binta Maci Bah, Chengcheng Song, Yifa Zhou, Li Ji, Ye Yuan   +6 more
doaj   +1 more source

Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases [PDF]

, 2022
Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently ...
Wiemann, Mathias
core  

Increasing the transglycosylation activity of alpha-galactosidase from Bifidobacterium adolescentis DSM 20083 by site-directed mutagenesis

, 2006
The ¿-galactosidase (AGA) from Bifidobacterium adolescentis DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme-substrate
Schipperus, R., Vincken, J.P., van den Broek, L.A.M., Voragen, A.G.J., Hinz, S.W.A., Doeswijk-Voragen, C.H.L.   +5 more
core   +2 more sources