Visual and rapid identification of Chlamydia trachomatis and Neisseria gonorrhoeae using multiplex loop-mediated isothermal amplification and a gold nanoparticle-based lateral flow biosensor [PDF]
Frontiers in Cellular and Infection Microbiology, 2023 Sexually transmitted chlamydia and gonorrhea infections caused by the bacteria Chlamydia trachomatis and Neisseria gonorrhoeae remain a major public health concern worldwide, particularly in less developed nations.
Xu Chen +6 more
doaj +5 more sources
Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor [PDF]
Diagnostics, 2019 Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance.
Flora M. Yrad +2 more
doaj +6 more sources
Rapid and Visual Detection of SARS-CoV-2 Using Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Linked With Gold Nanoparticle-Based Lateral Flow Biosensor [PDF]
Frontiers in Cellular and Infection Microbiology, 2021 BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has caused the outbreak of coronavirus disease 2019 (COVID-19) all over the world.
Xu Chen +9 more
doaj +6 more sources
Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions.
Xu Chen +6 more
doaj +5 more sources
Multiple Single-Nucleotide Polymorphism Detection for Antimalarial Pyrimethamine Resistance via Allele-Specific PCR Coupled with Gold Nanoparticle-Based Lateral Flow Biosensor. [PDF]
Antimicrob Agents Chemother, 2021 Molecular genotyping holds tremendous potential to detect antimalarial drug resistance (ADR) related to single nucleotide polymorphisms (SNPs). However, it relies on the use of complicated procedures and expensive instruments.
Jiang T +9 more
europepmc +5 more sources
Multiple Cross Displacement Amplification Combined with Gold Nanoparticle-Based Lateral Flow Biosensor for Detection of Vibrio parahaemolyticus. [PDF]
Front Microbiol, 2016 Vibrio parahaemolyticus (V. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. In the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (MCDA-LFB) to detect the toxR gene of V. parahaemolyticus in DNA extracts from pure cultures and spiked oyster
Wang Y +6 more
europepmc +5 more sources
A sequence-specific, nanoparticle-based biosensor platform for rapid and visual identification of serum hepatitis B virus pregenomic RNA in chronic hepatitis B patients [PDF]
Microbiology SpectrumChronic hepatitis B virus (HBV) infection is a major cause of liver-related morbidity and mortality worldwide. Serum HBV pregenomic RNA (pgRNA) is a surrogate marker for the transcriptional activity of covalently closed circular DNA.
Yuanyuan Gu +6 more
doaj +3 more sources
Nanoparticle-based biosensors coupled with loop-mediated isothermal amplification for rapid, visual, and in-field identification of pathogenic Leptospira [PDF]
BMC Infectious DiseasesBackground Leptospirosis is a worldwide re-emerging zoonotic infectious disease caused by pathogenic spirochetes of the Leptospira genus. The clinical symptoms at leptospirosis onset are similar to those of other febrile illnesses, and early etiological ...
Ling Xie +4 more
doaj +3 more sources
Biosensors, 2022 Flavivirus detection in humans and mosquito reservoirs has been an important issue since it can cause a variety of illnesses and could represent a health problem in geographical zones where the vector is endemic.
Cynthia Martinez-Liu +11 more
doaj +2 more sources
Rapid and sensitive detection of Pseudomonas aeruginosa using multiple cross displacement amplification and gold nanoparticle-based lateral flow biosensor visualization [PDF]
FEMS Microbiology Letters, 2018 Pseudomonas aeruginosa causes nosocomial infections of burn patients and other immunocompromised individuals, but the conventional diagnosis of P. aeruginosa infection depends on time-consuming culture-based methods. Hence, a simple, fast, sensitive technique for detection of P. aeruginosa using multiple cross displacement amplification (MCDA) and gold
Fan, Zhao +9 more
openaire +3 more sources