Results 1 to 10 of about 88,561 (112)

Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) method and real-tme fluorescence technique for ...
Chunrong Sun, Nan Jia, Xiaolan Huang, Fei Xiao, Juan Zhou, Yu Zhang, Jin Fu, Zheng Xu, Dong Qu, Xiaodai Cui, Yi Wang   +10 more
doaj   +4 more sources

Real-time fluorescent multiple cross displacement amplification for rapid and sensitive Mycoplasma pneumoniae detection [PDF]

Frontiers in Cellular and Infection Microbiology
Mycoplasma pneumoniae is a significant pathogen responsible for community-acquired pneumonia, predominantly affecting children and adolescents. Here, we devised a rapid method for M.
Fei Xiao, Yu Zhang, Wenjian Xu, Jin Fu, Xiaolan Huang, Nan Jia, Chunrong Sun, Zheng Xu, Baoying Zheng, Juan Zhou, Yi Wang, Lihui Meng   +11 more
doaj   +4 more sources

Multiple Cross Displacement Amplification Combined With Real-Time Polymerase Chain Reaction Platform: A Rapid, Sensitive Method to Detect Mycobacterium tuberculosis [PDF]

Frontiers in Microbiology, 2021
In this study, we evaluated the diagnostic accuracy of multiple cross displacement amplification (MCDA) combined with real-time PCR platform in pulmonary tuberculosis (PTB) patients. Total 228 PTB patients and 141 non-TB cases were enrolled. Based on the
Wei-wei Jiao, Gui-rong Wang, Lin Sun, Jing Xiao, Jie-qiong Li, Ya-cui Wang, Shu-ting Quan, Hai-rong Huang, A-dong Shen, A-dong Shen   +9 more
doaj   +4 more sources

Visual and fluorescent detection of Moraxella catarrhalis by multiple cross displacement amplification-based assay [PDF]

Heliyon
Moraxella catarrhalis (M. catarrhalis), once considered as a harmless commensal bacterium, is now recognized as a significant pathogen associated with human mucosal infection.
Xiaolan Huang, Lei Yu, Chunrong Sun, Fei Xiao, Nan Jia, Min Chen, Yu Zhang, Jin Fu, Zheng Xu, Xiaodai Cui, Juan Zhou, Yi Wang   +11 more
doaj   +4 more sources

Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification [PDF]

Infectious Medicine
Background: Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) are significant causative agents of acute respiratory tract infections that are prevalent among school-based outbreaks across China. Rapid and accurate diagnosis is crucial for effective control
Xinbei Jia, Linglong Wan, Fei Xiao, Xiaolan Huang, Juan Zhou, Yi Wang, Jun Tai   +6 more
doaj   +4 more sources

Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of Helicobacter pylori [PDF]

Frontiers in Cellular and Infection Microbiology
Helicobacter pylori (H. pylori, HP), recognized globally as one of the most widespread bacteria, serves as primary etiological agent for numerous gastroduodenal diseases, highlighting the urgent need to develop rapid and sensitive diagnostic method for H.
Yanfei Chen, Juan Zhou, Jiao Wang, Xi He, Xiaolan Huang, Fei Xiao, Nan Jia, Yi Wang, Xuemei Zhong   +8 more
doaj   +4 more sources

Rapid and sensitive detection of Haemophilus influenzae using multiple cross displacement amplification combined with CRISPR-Cas12a-based biosensing system [PDF]

Heliyon
Haemophilus influenzae (H. influenzae, Hi) is an opportunistic bacterium that colonizes the upper respiratory tract of humans and frequently causes meningitis, pneumonia, sepsis, and other severe infections in children. Early and accurate detection of H.
Xinbei Jia, Wenjian Xu, Fei Xiao, Nan Jia, Xiaolan Huang, Yiqin Zhang, Juan Zhou, Yi Wang, Jun Tai   +8 more
doaj   +4 more sources

Development and Application of a Multiple Cross Displacement Amplification Combined With Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Candida tropicalis [PDF]

Frontiers in Microbiology, 2021
Candida tropicalis is an increasingly opportunistic pathogen that causes serious invasive candidiasis threatening a patient’s life. Traditional methods to detect C. tropicalis infection depends on time-consuming, culture-based gold-standard methods.
Yu Wang, Yu Wang, Xue Zhao, Jinzhi Cheng, Xiaomin Tang, Xu Chen, Honglan Yu, Shijun Li   +7 more
doaj   +4 more sources

Development and Clinical Validation of Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Biosensor for Detection of Mycobacterium tuberculosis: Preliminary Results [PDF]

Frontiers in Microbiology, 2019
Tuberculosis is still a major threat to global public health. Here, a novel diagnosis assay, termed as multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor (MCDA-LFB), was developed to simultaneously detect ...
Wei-Wei Jiao, Yi Wang, Gui-Rong Wang, Ya-Cui Wang, Jing Xiao, Lin Sun, Jie-Qiong Li, Shu-An Wen, Ting-Ting Zhang, Qi Ma, Hai-Rong Huang, A-Dong Shen   +11 more
doaj   +4 more sources

Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor [PDF]

BMC Microbiology, 2022
Background Burkholderia pseudomallei (B. pseudomallei), as a highly pathogenic organism, causes melioidosis, which is a disease of public health importance in many tropical developing countries.
Xiaoxia Wang, Licheng Wang, Huaxiong Zhu, Chongzhen Wang, Xiong Zhu   +4 more
doaj   +2 more sources