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Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification [PDF]

open access: yesInfectious Medicine
Background: Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) are significant causative agents of acute respiratory tract infections that are prevalent among school-based outbreaks across China. Rapid and accurate diagnosis is crucial for effective control
Xinbei Jia   +6 more
doaj   +4 more sources

Visual and fluorescent detection of Moraxella catarrhalis by multiple cross displacement amplification-based assay [PDF]

open access: yesHeliyon
Moraxella catarrhalis (M. catarrhalis), once considered as a harmless commensal bacterium, is now recognized as a significant pathogen associated with human mucosal infection.
Xiaolan Huang   +11 more
doaj   +4 more sources

Rapid and sensitive detection of Haemophilus influenzae using multiple cross displacement amplification combined with CRISPR-Cas12a-based biosensing system [PDF]

open access: yesHeliyon
Haemophilus influenzae (H. influenzae, Hi) is an opportunistic bacterium that colonizes the upper respiratory tract of humans and frequently causes meningitis, pneumonia, sepsis, and other severe infections in children. Early and accurate detection of H.
Xinbei Jia   +8 more
doaj   +4 more sources

Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of Helicobacter pylori [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology
Helicobacter pylori (H. pylori, HP), recognized globally as one of the most widespread bacteria, serves as primary etiological agent for numerous gastroduodenal diseases, highlighting the urgent need to develop rapid and sensitive diagnostic method for H.
Yanfei Chen   +8 more
doaj   +4 more sources

Development and clinical evaluation of a real-time multiple cross displacement amplification assay for rapid and sensitive detection of Mycobacterium tuberculosis [PDF]

open access: yesHeliyon
Molecular techniques of nucleic acid testing recommended by the World Health Organization (WHO) for the Mycobacterium tuberculosis (MTB) detection were considered to have the potential access to the accurate tuberculosis (TB) notifications. In this study,
Chunrong Sun   +9 more
doaj   +4 more sources

Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) method and real-tme fluorescence technique for ...
Chunrong Sun   +10 more
doaj   +2 more sources

Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor [PDF]

open access: yesBMC Microbiology, 2022
Background Burkholderia pseudomallei (B. pseudomallei), as a highly pathogenic organism, causes melioidosis, which is a disease of public health importance in many tropical developing countries.
Xiaoxia Wang   +4 more
doaj   +2 more sources

Single Multiple Cross Displacement Amplification for Rapid and Real-Time Detection of Porcine Circovirus 3 [PDF]

open access: yesFrontiers in Veterinary Science, 2021
Since 2016, a novel porcine circovirus, PCV3, has been infecting pigs, causing significant economic losses to the pig industry. In recent years, the infection rate of PCV3 has been increasing, and thus rapid and accurate detection methods for PCV3 are ...
Zhibiao Bian   +39 more
doaj   +2 more sources

Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification [PDF]

open access: yesBMC Microbiology, 2023
Background Mycobacterium leprae (ML) is the pathogen that causes leprosy, which has a long history and still exists today. ML is an intracellular mycobacterium that dominantly induces leprosy by causing permanent damage to the skin, nerves, limbs and ...
Junfei Huang   +7 more
doaj   +2 more sources

Rapid Detection of Aspergillus fumigatus Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow [PDF]

open access: yesFrontiers in Cellular and Infection Microbiology, 2021
Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mold which can cause infection in the lungs, nose, eyes, brain, and bones in humans, especially in immunocompromised patients. However, it is difficult to diagnose A.
Luxi Jiang   +5 more
doaj   +2 more sources

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