Results 1 to 10 of about 16,381 (186)

Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae [PDF]

Frontiers in Cellular and Infection Microbiology, 2022
Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) method and real-tme fluorescence technique for ...
Chunrong Sun, Nan Jia, Xiaolan Huang, Fei Xiao, Juan Zhou, Yu Zhang, Jin Fu, Zheng Xu, Dong Qu, Xiaodai Cui, Yi Wang   +10 more
doaj   +6 more sources

Real-time fluorescent multiple cross displacement amplification for rapid and sensitive Mycoplasma pneumoniae detection [PDF]

Frontiers in Cellular and Infection Microbiology
Mycoplasma pneumoniae is a significant pathogen responsible for community-acquired pneumonia, predominantly affecting children and adolescents. Here, we devised a rapid method for M.
Fei Xiao, Yu Zhang, Wenjian Xu, Jin Fu, Xiaolan Huang, Nan Jia, Chunrong Sun, Zheng Xu, Baoying Zheng, Juan Zhou, Yi Wang, Lihui Meng   +11 more
doaj   +6 more sources

Rapid Detection of Aspergillus fumigatus Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow [PDF]

Frontiers in Cellular and Infection Microbiology, 2021
Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mold which can cause infection in the lungs, nose, eyes, brain, and bones in humans, especially in immunocompromised patients. However, it is difficult to diagnose A.
Luxi Jiang, Xiaomeng Li, Xiaomeng Li, Rumeng Gu, Rumeng Gu, Deguang Mu   +5 more
doaj   +7 more sources

Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of Helicobacter pylori [PDF]

Frontiers in Cellular and Infection Microbiology
Helicobacter pylori (H. pylori, HP), recognized globally as one of the most widespread bacteria, serves as primary etiological agent for numerous gastroduodenal diseases, highlighting the urgent need to develop rapid and sensitive diagnostic method for H.
Yanfei Chen, Juan Zhou, Jiao Wang, Xi He, Xiaolan Huang, Fei Xiao, Nan Jia, Yi Wang, Xuemei Zhong   +8 more
doaj   +6 more sources

Development and Application of a Multiple Cross Displacement Amplification Combined With Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Candida tropicalis [PDF]

Frontiers in Microbiology, 2021
Candida tropicalis is an increasingly opportunistic pathogen that causes serious invasive candidiasis threatening a patient’s life. Traditional methods to detect C. tropicalis infection depends on time-consuming, culture-based gold-standard methods.
Yu Wang, Yu Wang, Xue Zhao, Jinzhi Cheng, Xiaomin Tang, Xu Chen, Honglan Yu, Shijun Li   +7 more
doaj   +7 more sources

Highly Sensitive and Rapid Identification of Streptococcus agalactiae Based on Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor Assay [PDF]

Frontiers in Microbiology, 2020
Streptococccus agalactiae (S. agalactiae) is an important neonatal pathogen that is associated with mortality and morbidity. Therefore, we developed a rapid, accurate, and sensitive method based on multiple cross displacement amplification (MCDA) for the
Xueqin Cheng, Xueqin Cheng, Zhiqian Dou, Jing Yang, Yulong Gu, Dexi Liu, Ling Xie, Tao Ren, Yan Liu, Zhifang Yu, Yijun Tang, Meifang Wang   +11 more
doaj   +7 more sources

Development and Clinical Validation of Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Biosensor for Detection of Mycobacterium tuberculosis: Preliminary Results [PDF]

Frontiers in Microbiology, 2019
Tuberculosis is still a major threat to global public health. Here, a novel diagnosis assay, termed as multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor (MCDA-LFB), was developed to simultaneously detect ...
Wei-Wei Jiao, Yi Wang, Gui-Rong Wang, Ya-Cui Wang, Jing Xiao, Lin Sun, Jie-Qiong Li, Shu-An Wen, Ting-Ting Zhang, Qi Ma, Hai-Rong Huang, A-Dong Shen   +11 more
doaj   +7 more sources

Multiple Cross Displacement Amplification Combined With Real-Time Polymerase Chain Reaction Platform: A Rapid, Sensitive Method to Detect Mycobacterium tuberculosis [PDF]

Frontiers in Microbiology, 2021
In this study, we evaluated the diagnostic accuracy of multiple cross displacement amplification (MCDA) combined with real-time PCR platform in pulmonary tuberculosis (PTB) patients. Total 228 PTB patients and 141 non-TB cases were enrolled. Based on the
Wei-wei Jiao, Gui-rong Wang, Lin Sun, Jing Xiao, Jie-qiong Li, Ya-cui Wang, Shu-ting Quan, Hai-rong Huang, A-dong Shen, A-dong Shen   +9 more
doaj   +4 more sources

Ultrasensitive and highly specific detection of the Brucella genus and B. melitensis by CRISPR/Cas12b‐multiple cross displacement amplification technique [PDF]

Journal of Clinical Microbiology
Brucellosis is caused by members of the Brucella spp. and remains one of the world’s major zoonotic diseases. Brucella melitensis (B. melitensis) as the most contagious Brucella species cannot be ignored as an essential source of infection for ...
Sha Mao, Xinggui Yang, Yue Wang, Fengming Chen, Hai Jiang, Yi Wang, Yingqian Kang, Shijun Li   +7 more
doaj   +4 more sources

Multiple Cross Displacement Amplification Coupled With Gold Nanoparticles-Based Lateral Flow Biosensor for Detection of the Mobilized Colistin Resistance Gene mcr-1 [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Fast dissemination of the mobilized colistin resistance (mcr) gene mcr-1 in Enterobacteriaceae causes a huge threat to the treatment of severe infection. In the current report, a multiple cross displacement amplification (MCDA) coupled with the detection
Lin Gong, Ernan Liu, Jie Che, Juan Li, Xiaoli Liu, Huiqiong Xu, Jiansheng Liang   +6 more
doaj   +4 more sources