Results 31 to 40 of about 88,611 (123)

Development and clinical evaluation of a real-time multiple cross displacement amplification assay for rapid and sensitive detection of Mycobacterium tuberculosis

Heliyon
Molecular techniques of nucleic acid testing recommended by the World Health Organization (WHO) for the Mycobacterium tuberculosis (MTB) detection were considered to have the potential access to the accurate tuberculosis (TB) notifications. In this study,
Chunrong Sun, Chaohong Wang, Fei Xiao, Nan Jia, Xiaolan Huang, Jin Fu, Yu Zhang, Juan Zhou, Guirong Wang, Yi Wang   +9 more
doaj   +5 more sources

Development and comparison of novel multiple cross displacement amplification (MCDA) assays with other nucleic acid amplification methods for SARS-CoV-2 detection [PDF]

Scientific Reports, 2021
The development of alternative isothermal amplification assays including multiple cross displacement amplification (MCDA) may address speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection.
Laurence Don Wai Luu, Michael Payne, Xiaomei Zhang, Lijuan Luo, Ruiting Lan   +4 more
doaj   +2 more sources

Ultrasensitive and highly specific detection of the Brucella genus and B. melitensis by CRISPR/Cas12b‐multiple cross displacement amplification technique [PDF]

Journal of Clinical Microbiology
Brucellosis is caused by members of the Brucella spp. and remains one of the world’s major zoonotic diseases. Brucella melitensis (B. melitensis) as the most contagious Brucella species cannot be ignored as an essential source of infection for ...
Sha Mao, Xinggui Yang, Yue Wang, Fengming Chen, Hai Jiang, Yi Wang, Yingqian Kang, Shijun Li   +7 more
doaj   +2 more sources

Ultra-rapid and sensitive detection of African swine fever virus using multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor [PDF]

Frontiers in Microbiology
African swine fever (ASF) is a devastating disease that can kill almost all infected pigs, causing great damage to the pig industry and destabilizing the global economy.
Sha Mao, Sha Mao, Renjun Zhang, Xinggui Yang, Junfei Huang, Yingqian Kang, Yi Wang, Hong Chen, Shijun Li, Shijun Li   +9 more
doaj   +2 more sources

Rapid, highly specific and sensitive detection of human adenovirus using multiple cross displacement amplification coupled with CRISPR-Cas12a-based detection

Talanta Open
Human adenoviruses (HAdV), particularly serotypes 3 and 7, are significant pathogens that cause respiratory tract infection. Here, we developed a novel HAdV diagnostic assay, termed HAdV-MCDA-CRISPR, by combining multiple cross displacement amplification
Linglong Wan, Juan Zhou, Lei Yu, Xiaolan Huang, Jin Fu, Fei Xiao, Nan Jia, Yu Zhang, Min Chen, Xu Chen, Zhaomin Feng, Yi Wang, Lihui Meng   +12 more
doaj   +3 more sources

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Shigella spp. [PDF]

Frontiers in Microbiology, 2016
Shigella spp., the etiological agent of shigellosis or bacillary dysentery, are responsible for considerable morbidity and mortality in excess of a million deaths globally per year.
Yi Wang, Yan Wang, Jianguo Xu, Changyun Ye   +3 more
doaj   +3 more sources

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Listeria monocytogenes

International Journal of Nanomedicine, 2017
Yi Wang,1 Hui Li,1,2 Yan Wang,1 Hua Li,1 Lijuan Luo,1 Jianguo Xu,1 Changyun Ye1 1State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for ...
Wang Y, Li H, Wang Y, Li H, Luo L, Xu J, Ye C   +6 more
doaj   +4 more sources

Rapid Detection of Brucella spp. and Elimination of Carryover Using Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Brucella spp.is capable of causing disease in a range of animal hosts, and human brucellosis is regarded as a life-threating disease. A novel isothermal amplification technique, termed multiple cross displacement amplification (MCDA), was employed for ...
Shijun Li, Ying Liu, Yue Wang, Ming Wang, Chunting Liu, Yi Wang   +5 more
doaj   +2 more sources

Multiple Cross Displacement Amplification Coupled With Gold Nanoparticles-Based Lateral Flow Biosensor for Detection of the Mobilized Colistin Resistance Gene mcr-1 [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Fast dissemination of the mobilized colistin resistance (mcr) gene mcr-1 in Enterobacteriaceae causes a huge threat to the treatment of severe infection. In the current report, a multiple cross displacement amplification (MCDA) coupled with the detection
Lin Gong, Ernan Liu, Jie Che, Juan Li, Xiaoli Liu, Huiqiong Xu, Jiansheng Liang   +6 more
doaj   +2 more sources

Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of Mycoplasma pneumoniae [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Mycoplasma pneumoniae (M. pneumoniae) is responsible for pneumonia, and is a causative agent of other respiratory tract infections (e.g., bronchiolitis and tracheobronchitis). Herein, we established and applied a multiple cross displacement amplification
Yacui Wang, Yi Wang, Shuting Quan, Weiwei Jiao, Jieqiong Li, Lin Sun, Yonghong Wang, Xue Qi, Xingyun Wang, Adong Shen   +9 more
doaj   +2 more sources