Results 31 to 40 of about 16,381 (186)

A modified multiple cross displacement amplification linked with a gold nanoparticle biosensor for the detection of Epstein-Barr virus in clinical applications. [PDF]

Front Microbiol, 2023
Epstein-Barr virus (EBV), a double-stranded DNA virus belonging to the family Herpesviridae, infects more than 95% of healthy adults by attacking the host immune system. Here, a novel detection protocol, utilizing the modified multiple cross displacement amplification (MCDA) technique combined with a gold nanoparticles-based lateral flow biosensors ...
Zeng X, Yang X, Yang L, Yi X, Chen X, Huang J, Wang Y, Li S.   +7 more
europepmc   +5 more sources

Multiple Cross Displacement Amplification Combined with Real-Time Fluorescence Monitoring for Efficient, Specific, and Sensitive Neisseria meningitidis Detection [PDF]

ACS Omega
Invasive meningococcal disease, caused by Neisseria meningitidis (N. meningitidis), is a critical global health issue, necessitating swift and precise diagnostics for effective management and control. Here, we introduce a novel diagnostic assay, NM-RT-MCDA, that combines multiple cross displacement amplification (MCDA) with real-time fluorescence ...
Rui Huang, DaWei Huang, Chunrong Sun, Juan Zhou, Nan Jia, Fei Xiao, Xiaolan Huang, Shijun Li, Yi Wang   +8 more
doaj   +4 more sources

Development and comparison of novel multiple cross displacement amplification (MCDA) assays with other nucleic acid amplification methods for SARS-CoV-2 detection [PDF]

Scientific Reports, 2021
The development of alternative isothermal amplification assays including multiple cross displacement amplification (MCDA) may address speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection.
Laurence Don Wai Luu, Michael Payne, Xiaomei Zhang, Lijuan Luo, Ruiting Lan   +4 more
doaj   +2 more sources

Rapid detection of human adenovirus by multiple cross displacement amplification combined with nanoparticle-based biosensor platform

Sensors and Actuators Reports
Human adenoviruses (HAdV), particularly serotypes 3 and 7 (HAdV-3 and HAdV-7), are significant respiratory pathogens that contribute to high morbidity rates and severe pneumonia in infants and children.
Linglong Wan, Xiaolan Huang, Jin Fu, Fei Xiao, Nan Jia, Yu Zhang, Min Chen, Xiong Zhu, Shijun Li, Juan Zhou, Yi Wang   +10 more
doaj   +2 more sources

Ultra-rapid and sensitive detection of African swine fever virus using multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor [PDF]

Frontiers in Microbiology
African swine fever (ASF) is a devastating disease that can kill almost all infected pigs, causing great damage to the pig industry and destabilizing the global economy.
Sha Mao, Sha Mao, Renjun Zhang, Xinggui Yang, Junfei Huang, Yingqian Kang, Yi Wang, Hong Chen, Shijun Li, Shijun Li   +9 more
doaj   +2 more sources

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Listeria monocytogenes

International Journal of Nanomedicine, 2017
Yi Wang,1 Hui Li,1,2 Yan Wang,1 Hua Li,1 Lijuan Luo,1 Jianguo Xu,1 Changyun Ye1 1State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for ...
Wang Y, Li H, Wang Y, Li H, Luo L, Xu J, Ye C   +6 more
doaj   +4 more sources

Rapid Detection of Brucella spp. and Elimination of Carryover Using Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Brucella spp.is capable of causing disease in a range of animal hosts, and human brucellosis is regarded as a life-threating disease. A novel isothermal amplification technique, termed multiple cross displacement amplification (MCDA), was employed for ...
Shijun Li, Ying Liu, Yue Wang, Ming Wang, Chunting Liu, Yi Wang   +5 more
doaj   +2 more sources

Development of CRISPR/Cas12b-Based Multiple Cross Displacement Amplification Technique for the Detection of <i>Mycobacterium tuberculosis</i> Complex in Clinical Settings. [PDF]

Microbiol Spectr, 2023
The Mycobacterium tuberculosis complex pathogen is a crucial infectious agent of tuberculosis. Hence, improving the capability of MTC detection is one of the most urgently required strategies for preventing and controlling TB.
Yang X, Huang J, Chen Y, Ying X, Tan Q, Chen X, Zeng X, Lei S, Wang Y, Li S.   +9 more
europepmc   +3 more sources

A Nanoparticle-Based Biosensor Combined With Multiple Cross Displacement Amplification for the Rapid and Visual Diagnosis of Neisseria gonorrhoeae in Clinical Application. [PDF]

Front Microbiol, 2021
Gonorrhea is a sexually transmitted disease caused by the host-adapted human pathogen, Neisseria gonorrhoeae. The morbidity is increasing and poses a major public health concern, especially in resource-scarce regions. Therefore, a rapid, visual, sensitive, specific, cost-saving, and simple assay for N.
Chen X, Huang L, Zhou Q, Tan Y, Tan X, Dong S.   +5 more
europepmc   +5 more sources

A Novel Detection of Enterococcus faecalis Using Multiple Cross Displacement Amplification Linked with Gold Nanoparticle Lateral Flow Biosensor

Infection and Drug Resistance, 2019
Xu Chen,1,2 Kai Ma,3 Xu Yi,1 Ziyu Xiao,4 Lijuan Xiong,3 Yu Wang,5 Shijun Li2 1Central Laboratory of the Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550003, People’s Republic of China; 2Laboratory
Chen X, Ma K, Yi X, Xiao Z, Xiong L, Wang Y, Li S   +6 more
doaj   +4 more sources