Results 41 to 50 of about 16,396 (200)

Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of Mycoplasma pneumoniae [PDF]

Frontiers in Cellular and Infection Microbiology, 2019
Mycoplasma pneumoniae (M. pneumoniae) is responsible for pneumonia, and is a causative agent of other respiratory tract infections (e.g., bronchiolitis and tracheobronchitis). Herein, we established and applied a multiple cross displacement amplification
Yacui Wang, Yi Wang, Shuting Quan, Weiwei Jiao, Jieqiong Li, Lin Sun, Yonghong Wang, Xue Qi, Xingyun Wang, Adong Shen   +9 more
doaj   +2 more sources

Development and clinical evaluation of a real-time multiple cross displacement amplification assay for rapid and sensitive detection of Mycobacterium tuberculosis

Heliyon
Molecular techniques of nucleic acid testing recommended by the World Health Organization (WHO) for the Mycobacterium tuberculosis (MTB) detection were considered to have the potential access to the accurate tuberculosis (TB) notifications. In this study,
Chunrong Sun, Chaohong Wang, Fei Xiao, Nan Jia, Xiaolan Huang, Jin Fu, Yu Zhang, Juan Zhou, Guirong Wang, Yi Wang   +9 more
doaj   +3 more sources

A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application

Frontiers in Cellular and Infection Microbiology, 2023
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a ...
Nan Jia, Chaohong Wang, Xiaming Liu, Xiaolan Huang, Fei Xiao, Jin Fu, Chunrong Sun, Zheng Xu, Guirong Wang, Juan Zhou, Yi Wang   +10 more
doaj   +1 more source

Biosensor-Based Multiple Cross Displacement Amplification for the Rapid Detection of Mycobacterium leprae

, 2023
Leprosy is an ancient disease caused by Mycobacterium leprae (ML) that remains a public health problem in poverty-stricken areas worldwide. Although many ML detection techniques have been used, a rapid and sensitive tool is essential for the early ...
Shijun Li (165293), Yi Tong, Xiaoyu Wei, Xinggui Yang (11216037), Junfei Huang, Yijiang Chen, Xu Chen (432759), Xinggui Yang, Shijun Li, Xiaoyu Wei (2794636), Yi Tong (45623), Jinlan Li, Junfei Huang (9764927), Yijiang Chen (102749), Jinlan Li (7510904), Xu Chen   +15 more
core   +1 more source

Establishment and Application of Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor (MCDA-LFB) for Visual and Rapid Detection of Candida albicans in Clinical Samples. [PDF]

Front Cell Infect Microbiol, 2019
Candida albicans is an opportunistic pathogenic yeast that predominantly causes invasive candidiasis. The conventional diagnosis of C. albicans infection depends on time-consuming, culture-based gold-standard methods.
Zhao F, Niu L, Yan L, Nong J, Wang C, Wang J, Gao N, Zhu X, Wu L, Zheng F, Hu S.   +10 more
europepmc   +2 more sources

A CRISPR-Cas12a—Based platform for ultrasensitive, rapid, and highly specific detection of Mycoplasma pneumonia in clinical application

Frontiers in Bioengineering and Biotechnology, 2023
Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based ...
Nan Jia, Juan Zhou, Fei Xiao, Baoying Zheng, Xiaolan Huang, Chunrong Sun, Jin Fu, Zheng Xu, Min Chen, Yi Wang   +9 more
doaj   +1 more source

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

Frontiers in Bioengineering and Biotechnology, 2021
Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute and chronic liver diseases in humans. Genotyping plays an important role in determining clinical outcomes and response to antiviral treatment in HBV–infected ...
Xu Chen, Yan Tan, Shuoshi Wang, Xueli Wu, Rui Liu, Xinggui Yang, Yi Wang, Jun Tai, Shijun Li   +8 more
doaj   +1 more source

Table_1_A Nanoparticle-Based Biosensor Combined With Multiple Cross Displacement Amplification for the Rapid and Visual Diagnosis of Neisseria gonorrhoeae in Clinical Application.docx

, 2021
Gonorrhea is a sexually transmitted disease caused by the host-adapted human pathogen, Neisseria gonorrhoeae. The morbidity is increasing and poses a major public health concern, especially in resource-scarce regions.
Xuhong Tan (11562247), Shilei Dong (11130447), Liming Huang (110579), Qingxue Zhou (11130444), Xu Chen (432759), Yan Tan (131762)   +5 more
core   +1 more source

Highly Sensitive and Specific Detection and Serotyping of Five Prevalent Salmonella Serovars by Multiple Cross-Displacement Amplification

, 2020
Salmonella is a common cause of foodborne disease worldwide, including Australia. More than 85% of outbreaks of human salmonellosis in Australia were caused by five Salmonella serovars. Rapid, accurate, and sensitive identification of Salmonella serovars
Payne, M ; https://orcid.org/, Sintchenko, V, Wang, Q, Zhang, X, Lan, R ; https://orcid.org/   +4 more
core   +1 more source

Image_1_Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use.tif

, 2022
Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions.
Wei Yuan (64653), figshare admin frontiersin (4545187), Shilei Dong (11130447), Qingxue Zhou (11130444), Xu Chen (432759), Ronghua Wang (1834945), Yan Tan (131762)   +6 more
core   +1 more source