Results 41 to 50 of about 16,381 (186)

Highly Sensitive and Specific Detection and Serotyping of Five Prevalent Salmonella Serovars by Multiple Cross-Displacement Amplification [PDF]

The Journal of Molecular Diagnostics, 2020
Salmonella is a common cause of foodborne disease worldwide, including Australia. More than 85% of outbreaks of human salmonellosis in Australia were caused by five Salmonella serovars. Rapid, accurate, and sensitive identification of Salmonella serovars is vital for diagnosis and public health surveillance.
Zhang, X, Payne, M, Wang, Q, Sintchenko, V, Lan, R   +4 more
openaire   +3 more sources

Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect Neisseria meningitidis. [PDF]

Infect Drug Resist, 2019
Neisseria meningitidis is a leading pathogen of meningococcal disease in humans worldwide. Multiple cross displacement mplification (MCDA) combined with nanoparticles-based lateral flow biosensor (MCDA-LFB) has been reported for the rapid detection of several bacterial pathogens in recent years.
Li S, Liu C, Liu Y, Ma Q, Wang Y, Wang Y.   +5 more
europepmc   +5 more sources

Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification [PDF]

Scientific Reports, 2015
AbstractWe have devised a novel amplification strategy based on isothermal strand-displacement polymerization reaction, which was termed multiple cross displacement amplification (MCDA). The approach employed a set of ten specially designed primers spanning ten distinct regions of target sequence and was preceded at a constant temperature (61–65 °C ...
Yi Wang, Yan Wang, Ai-Jing Ma, Dong-Xun Li, Li-Juan Luo, Dong-Xin Liu, Dong Jin, Kai Liu, Chang-Yun Ye   +8 more
openaire   +2 more sources

A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application

Frontiers in Cellular and Infection Microbiology, 2023
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a ...
Nan Jia, Chaohong Wang, Xiaming Liu, Xiaolan Huang, Fei Xiao, Jin Fu, Chunrong Sun, Zheng Xu, Guirong Wang, Juan Zhou, Yi Wang   +10 more
doaj   +1 more source

Highly sensitive and specific diagnosis of COVID-19 by reverse transcription multiple cross-displacement amplification-labelled nanoparticles biosensor. [PDF]

Eur Respir J, 2020
BackgroundThe ongoing outbreak of the novel human coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (also known as 2019-nCoV) has become a global health concern. Rapid and easy-to-use diagnostic techniques are urgently needed to diagnose SARS-CoV-2 infection.MethodsWe devised a reverse transcription multiple cross-displacement ...
Li S, Jiang W, Huang J, Liu Y, Ren L, Zhuang L, Zheng Q, Wang M, Yang R, Zeng Y, Wang Y.   +10 more
europepmc   +4 more sources

A CRISPR-Cas12a—Based platform for ultrasensitive, rapid, and highly specific detection of Mycoplasma pneumonia in clinical application

Frontiers in Bioengineering and Biotechnology, 2023
Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based ...
Nan Jia, Juan Zhou, Fei Xiao, Baoying Zheng, Xiaolan Huang, Chunrong Sun, Jin Fu, Zheng Xu, Min Chen, Yi Wang   +9 more
doaj   +1 more source

Development and comparison of a novel multiple cross displacement amplification (MCDA) assay with other nucleic acid amplification methods for SARS-CoV-2 detection [PDF]

Scientific Reports, 2020
Abstract The development of alternative isothermal amplification assays including multiple cross displacement amplification (MCDA) may address speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection.
Wai Luu LD, Payne M, Zhang X, Luo L, Lan R.   +4 more
europepmc   +6 more sources

Multiple Cross Displacement Amplification Combined with Gold Nanoparticle-Based Lateral Flow Biosensor for Detection of Vibrio parahaemolyticus. [PDF]

Front Microbiol, 2016
Vibrio parahaemolyticus (V. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. In the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (MCDA-LFB) to detect the toxR gene of V. parahaemolyticus in DNA extracts from pure cultures and spiked oyster
Wang Y, Li H, Li D, Li K, Wang Y, Xu J, Ye C.   +6 more
europepmc   +4 more sources

Multiplex, rapid and sensitive isothermal detection of nucleic-acid sequence by endonuclease restriction-mediated real-time multiple cross displacement amplification

Frontiers in Microbiology, 2016
We have devised a novel isothermal amplification technology, termed endonuclease restriction-mediated real-time multiple cross displacement amplification (ET-MCDA), which facilitated multiplex, rapid, specific and sensitive detection of nucleic-acid ...
Yi eWang, Yan eWang, Lu eZhang, Dongxin eLiu, Lijuan eLuo, Hua eLi, Xiaolong eCao, Xiaolong eCao, Kai eLiu, Jianguo eXu, Changyun eYe   +10 more
doaj   +1 more source

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

Frontiers in Bioengineering and Biotechnology, 2021
Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute and chronic liver diseases in humans. Genotyping plays an important role in determining clinical outcomes and response to antiviral treatment in HBV–infected ...
Xu Chen, Yan Tan, Shuoshi Wang, Xueli Wu, Rui Liu, Xinggui Yang, Yi Wang, Jun Tai, Shijun Li   +8 more
doaj   +1 more source