Results 21 to 30 of about 8,806,112 (203)

Multiplex, rapid and sensitive isothermal detection of nucleic-acid sequence by endonuclease restriction-mediated real-time multiple cross displacement amplification [PDF]

Frontiers in Microbiology, 2016
We have devised a novel isothermal amplification technology, termed endonuclease restriction-mediated real-time multiple cross displacement amplification (ET-MCDA), which facilitated multiplex, rapid, specific and sensitive detection of nucleic-acid ...
Yi eWang, Yan eWang, Lu eZhang, Dongxin eLiu, Lijuan eLuo, Hua eLi, Xiaolong eCao, Xiaolong eCao, Kai eLiu, Jianguo eXu, Changyun eYe   +10 more
doaj   +2 more sources

Highly sensitive and rapid determination of Coxsackievirus A16 using restriction endonuclease‐mediated reverse transcription multiple cross displacement amplification [PDF]

BMC Microbiology
Background As a major etiological agent of hand, foot, and mouth disease in humans, Coxsackievirus A16 (CVA16) exerts a detrimental impact on the health of infants and toddlers.
Qi Liang, Yumei Cao, Xujian Zhang, Rui Ye, Mao Liu, Shu Zhang, Yu Wang   +6 more
doaj   +2 more sources

Rapid and ultrasensitive detection of Candida tropicalis using multiple cross-displacement amplification combined with CRISPR/Cas12a [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundCandida tropicalis (C. tropicalis), classified as a World Health Organization “critical priority” pathogen, causes life-threatening invasive infections with high mortality due to diagnostic delays.
Rui Ye, Rui Ye, Rui Ye, Mao Liu, Mao Liu, Mao Liu, Xujian Zhang, Qi Liang, Qi Liang, Qi Liang, Yumei Cao, Chuiyu Zhu, Honglan Yu, Shu Zhang, Shu Zhang, Yu Wang, Yu Wang   +16 more
doaj   +2 more sources

Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex [PDF]

BMC Microbiology, 2021
Background Tuberculosis (TB) is a serious chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC). Hence, the development of a novel, simple, rapid and sensitive method to detect MTBC is of great significance for the prevention and
Junfei Huang, Ziyu Xiao, Xinggui Yang, Xu Chen, Xiaojuan Wang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shijun Li   +9 more
doaj   +2 more sources

Development and Application of a Multiple Cross Displacement Amplification Combined With Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Candida tropicalis [PDF]

Frontiers in Microbiology, 2021
Candida tropicalis is an increasingly opportunistic pathogen that causes serious invasive candidiasis threatening a patient’s life. Traditional methods to detect C. tropicalis infection depends on time-consuming, culture-based gold-standard methods.
Yu Wang, Yu Wang, Xue Zhao, Jinzhi Cheng, Xiaomin Tang, Xu Chen, Honglan Yu, Shijun Li   +7 more
doaj   +2 more sources

Rapid detection of human adenovirus by multiple cross displacement amplification combined with nanoparticle-based biosensor platform

Sensors and Actuators Reports
Human adenoviruses (HAdV), particularly serotypes 3 and 7 (HAdV-3 and HAdV-7), are significant respiratory pathogens that contribute to high morbidity rates and severe pneumonia in infants and children.
Linglong Wan, Xiaolan Huang, Jin Fu, Fei Xiao, Nan Jia, Yu Zhang, Min Chen, Xiong Zhu, Shijun Li, Juan Zhou, Yi Wang   +10 more
doaj   +3 more sources

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Listeria monocytogenes

International Journal of Nanomedicine, 2017
Yi Wang,1 Hui Li,1,2 Yan Wang,1 Hua Li,1 Lijuan Luo,1 Jianguo Xu,1 Changyun Ye1 1State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for ...
Wang Y, Li H, Wang Y, Li H, Luo L, Xu J, Ye C   +6 more
doaj   +1 more source

The use of Multiple Displacement Amplification to increase the detection and genotyping of Trypanosoma samples immobilised on FTA filters. [PDF]

, 2007
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias.
Sweeney, L., Turner, C.M.R., Morrison, L.J., Likeufack, A.C.L., Truc, P., Tait, A., McCormack, G., MacLeod, A.   +7 more
core   +9 more sources

Multiple cross displacement amplification-a more applicable technique in detecting Pseudomonas aeruginosa of ventilator-associated pneumonia (VAP) [PDF]

Critical Care, 2020
Background Early and rapid identification of Pseudomonas aeruginosa (P. aeruginosa) in patients with suspected ventilator-associated pneumonia (VAP) provides theoretical clinical advantages in therapeutic optimization strategies.
Juxiang Wang, Huimin Chen, Xiaomin Lin, Chengyi Ji, Bin Chen   +4 more
doaj   +2 more sources

Multiple cross displacement amplification coupled with a lateral flow biosensor for ultra-rapid and highly sensitive detection of Escherichia coli in neonatal sepsis [PDF]

Microbiology Spectrum
Escherichia coli (E. coli) is a predominant pathogen causing neonatal sepsis, and rapid pathogen identification is critical for timely antimicrobial therapy.
Ruoyi Xiao, Peicen Zou, Juan Zhou, Ying Li, Ying Chen, Pan Huang, Yi Wang, Yajuan Wang   +7 more
doaj   +2 more sources