Results 21 to 30 of about 16,381 (186)

Multiple cross displacement amplification-a more applicable technique in detecting Pseudomonas aeruginosa of ventilator-associated pneumonia (VAP) [PDF]

Critical Care, 2020
Background Early and rapid identification of Pseudomonas aeruginosa (P. aeruginosa) in patients with suspected ventilator-associated pneumonia (VAP) provides theoretical clinical advantages in therapeutic optimization strategies.
Juxiang Wang, Huimin Chen, Xiaomin Lin, Chengyi Ji, Bin Chen   +4 more
doaj   +8 more sources

Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification [PDF]

Infectious Medicine
Background: Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) are significant causative agents of acute respiratory tract infections that are prevalent among school-based outbreaks across China. Rapid and accurate diagnosis is crucial for effective control
Xinbei Jia, Linglong Wan, Fei Xiao, Xiaolan Huang, Juan Zhou, Yi Wang, Jun Tai   +6 more
doaj   +4 more sources

Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis [PDF]

BMC Microbiology, 2020
Background Bordetella pertussis is the causative agent of pertussis, a respiratory tract infectious disease. Efficient techniques for detection of B. pertussis isolates are important for clinical diagnosis. Multiple cross displacement amplification (MCDA)
Shijun Li, Chunting Liu, Ying Liu, Qing Ma, Yue Wang, Yi Wang   +5 more
doaj   +5 more sources

Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex [PDF]

BMC Microbiology, 2021
Background Tuberculosis (TB) is a serious chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC). Hence, the development of a novel, simple, rapid and sensitive method to detect MTBC is of great significance for the prevention and
Junfei Huang, Ziyu Xiao, Xinggui Yang, Xu Chen, Xiaojuan Wang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shijun Li   +9 more
doaj   +6 more sources

Rapid and sensitive detection of Haemophilus influenzae using multiple cross displacement amplification combined with CRISPR-Cas12a-based biosensing system [PDF]

Heliyon
Haemophilus influenzae (H. influenzae, Hi) is an opportunistic bacterium that colonizes the upper respiratory tract of humans and frequently causes meningitis, pneumonia, sepsis, and other severe infections in children. Early and accurate detection of H.
Xinbei Jia, Wenjian Xu, Fei Xiao, Nan Jia, Xiaolan Huang, Yiqin Zhang, Juan Zhou, Yi Wang, Jun Tai   +8 more
doaj   +4 more sources

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of Shigella spp. [PDF]

Frontiers in Microbiology, 2016
Shigella spp., the etiological agent of shigellosis or bacillary dysentery, are responsible for considerable morbidity and mortality in excess of a million deaths globally per year.
Yi Wang, Yan Wang, Jianguo Xu, Changyun Ye   +3 more
doaj   +5 more sources

Visual and rapid detection of Acinetobacter baumannii by a multiple cross displacement amplification combined with nanoparticles-based biosensor assay [PDF]

AMB Express, 2019
The traditional microbiological methods used for detecting Acinetobacter baumannii were usually time-consuming and labor-intensive. Thus, we sought to establish a novel rapid detecting method for target pathogen.
Xueqin Cheng, Jing Yang, Meifang Wang, Peng Wu, Qiong Du, Jinjuan He, Yijun Tang   +6 more
doaj   +5 more sources

Development and clinical evaluation of a real-time multiple cross displacement amplification assay for rapid and sensitive detection of Mycobacterium tuberculosis [PDF]

Heliyon
Molecular techniques of nucleic acid testing recommended by the World Health Organization (WHO) for the Mycobacterium tuberculosis (MTB) detection were considered to have the potential access to the accurate tuberculosis (TB) notifications. In this study,
Chunrong Sun, Chaohong Wang, Fei Xiao, Nan Jia, Xiaolan Huang, Jin Fu, Yu Zhang, Juan Zhou, Guirong Wang, Yi Wang   +9 more
doaj   +4 more sources

Isothermal amplification and rapid detection of Klebsiella pneumoniae based on the multiple cross displacement amplification (MCDA) and gold nanoparticle lateral flow biosensor (LFB). [PDF]

PLoS ONE, 2018
Klebsiella pneumoniae (K. pneumoniae) is a frequent pathogen causing nosocomial infections and outbreaks. We developed a multiple cross displacement amplification (MCDA) assay for the detection of K.
Lina Niu, Fan Zhao, Jinlong Chen, Jinqing Nong, Chunmei Wang, Jing Wang, Naishu Gao, Xiaoxue Zhu, Lei Wu, Shoukui Hu   +9 more
doaj   +5 more sources

Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes [PDF]

BMC Microbiology
Background S. pyogenes, is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and streptococcal toxic shock syndrome (STSS), often resulting in high mortality rates.
Zhiqian Dou, Ling Xie, Meiling Gao, Dexi Liu   +3 more
doaj   +4 more sources