Results 51 to 60 of about 3,568 (132)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Mycotoxin management in the cereal supply chain: Advanced detection, detoxification, and smart packaging strategies

Journal of the Science of Food and Agriculture, EarlyView.
Abstract Mycotoxins remain a persistent threat to the safety and quality of cereal grains and other agricultural products, and their impact on human health continues to raise global concerns. In many situations, the practices traditionally used to control these toxins are no longer sufficiently effective. They can be costly, difficult to implement on a
Abolfazl Asqardokht‐Aliabadi, Dipendra Kumar Mahato, Jafar Mohammadzadeh Milani   +2 more
wiley   +1 more source

Analysis of Carbohydrates and Glycoconjugates by Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2023–2024

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The use of MALDI mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this comprehensive review is the twelfth update of the original article published in 1999 and brings coverage of the literature to the end of 2024.
David J. Harvey
wiley   +1 more source

Peptide‐based antibacterial nanoplatforms: Design principles, stimuli‐regulated behaviors, and applications

Responsive Materials, EarlyView.
Peptide‐based antibacterial nanoplatforms, encompassing self‐assembled peptides and peptide‐engineered inorganic, polymeric, and lipid nanocarriers, are systematically reviewed. The article highlights design principles and stimuli‐responsive regulation for improving peptide stability, delivery efficiency, and antibacterial performance.
Peng Tan, Chenlong Zhou, Shuaikang Yang, Zhenduo Chen, Guanghui Zhao, Qi Tang, Haoyu Yan, Feng Li, Xi Ma   +8 more
wiley   +1 more source

Microfluidic Molecular Diagnostic Chips: Functional Modules, Integration Strategies, and Emerging Applications

Small Methods, EarlyView.
This comprehensive review delivers an engineering‐centric analysis of microfluidic molecular diagnostic chips, breaking them down into foundational modules from fluid manipulation to bio‐sensing. Beyond chip design, it evaluates sample‐to‐answer integration strategies and commercial readiness levels.
Donghoon Koo, Egor Kokin, Sangyul Baik, Chanyong Park, Minji Kim, Jeongmin Kim, Ryungeun Song, Hosub Lim   +7 more
wiley   +1 more source

Two‐Dimensional Materials For Lateral Flow Assays: A Critical Review of Functional Roles and Translational Challenges

VIEW, EarlyView.
2D nanosheets, with large surface area, excellent optical/electrical properties, and high specificity, combine with lateral flow assay strips to enable rapid, readable detection, boosting the strips’ development and applications. Abstract Lateral flow assays (LFAs) are widely used for point‐of‐care diagnostics, but their detection capability remains ...
Weimin Xu, Ruping Liu, Na Gao, Jianxiao Zhu, Haowen Jiang, Shouzheng Jiao, Zhicheng Sun, Kai Shen, Gaolian Xu, Minghua Du, Ziyan Li, Yang Wang   +11 more
wiley   +1 more source

Nanobodies as next‐generation targeting platforms: From discovery technologies to translational biomedicine

VIEW, EarlyView.
Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley   +1 more source

Strategies for enhancing sensitivity in lateral flow assays

VIEW, EarlyView.
Lateral flow assays (LFAs) are essential for cost‐effective, rapid point‐of‐care diagnostics. However, conventional colourimetric LFAs often lack the sensitivity required for low‐abundance biomarkers. This review evaluates six enhancement strategies: flow modulation, sample preconcentration, advanced reporters, chemical signal amplification, structural
Aylar Eslami Saed, Jacopo Giaretta, Syamak Farajikhah, Fariba Dehghani   +3 more
wiley   +1 more source

Electrochemical aptasensors for ultrasensitive multiplexed detection: Towards a new era of precision diagnostics and environmental monitoring

VIEW, EarlyView.
Electrochemical aptasensors are emerging as powerful platforms for precision diagnostics and environmental monitoring, owing to their high selectivity, programmability and compatibility with miniaturised systems. However, their broader application is constrained by limited signal intensity and challenges in reliable multiplexed detection.
Azadeh Abdi, Ngoan Thi Thao Nguyen, Riccardo Zulli, Jacopo Giaretta, Fariba Dehghani, Syamak Farajikhah   +5 more
wiley   +1 more source

Nanoparticles in Periodontology and Implant Dentistry: From Mechanisms to Clinical Applications

Journal of Periodontal Research, EarlyView.
Nanotechnology holds significant promise in improving dental care, yet there is a need for more reliable clinical studies to validate its effectiveness and safety. ABSTRACT Recent advances in nanotechnology are reshaping the landscape of periodontology and implant dentistry, particularly through the application of nanoparticles (NPs).
Chun Xu, Yuan Chen, Mario Romandini, Håvard Jostein Haugen, Sašo Ivanovski   +4 more
wiley   +1 more source