Results 131 to 140 of about 1,353,787 (301)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Optimization and Visualization of Industrial Production Knowledge Graph Based on BERT and RGCN

Journal of Engineering, Project, and Production Management
To improve the accuracy and efficiency of existing methods for optimizing and visualizing multivariate data knowledge graphs, a new model is proposed.
Shucheng Li
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Software system for simulation of study of queuing system with heterogeneous servers and independent queues

Vestnik Samarskogo Gosudarstvennogo Tehničeskogo Universiteta. Seriâ: Fiziko-Matematičeskie Nauki, 2013
Software package for simulation of study and automated visualization of the state orgraph of queuing system with heterogeneous servers and independent limited queues is described. The base of the algorithm is the method of description of such queues with
Andrey P Kotenko, Maksim B Bukarenko
doaj  

GraphXML - An XML-Based Graph Description Format

, 2001
GraphXML is a graph description language in XML that can be used as an interchange format for graph drawing and visualization packages. The generality and rich features of XML make it possible to define an interchange format that not only supports the ...
Herman, Ivan, Marshall, M. Scott, Ivan Herman, M. Scott Marshall   +3 more
core   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Land Use Thematic Maps Recommendation Based on Pan-Map Visualization Dimension Theory

Land
In the era of information and communication technology (ICT), the advancement of science and technology has led to a trend of diversification in map representation.
Yebin Chen, Zhicheng Shi, Yaxing Li, Dezhi Han, Minmin Li, Zhigang Zhao   +5 more
doaj   +1 more source

Graph visualization using force algorithms [PDF]

, 2016
This article describes theory and realization of force-based graph visualization. There is an overview of aesthetic criteria of visualization. Article contains examples of visualization using force-based algorithms and describes the need to use different
Демешко, М. В., Дёмин, Антон Юрьевич   +1 more
core  

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source