Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Graphical method for convolutional codes representation [PDF]
, 2007 Graphical method for convolutional codes description is proposed giving pictorial presentation of construction principles for these codes, allowing to construct codes without determination of generator polynomial and to perform comparative analysis
Дяченко, О.Н.
core
A graphical method for testing the equality of several variances
, 1997 The problem of testing the equality of several variances arises in many areas. For testing the equality of variances, several tests are available in the literature which demonstrate only the statistical significance of the variances.
C. V. Rao, S. Hari Krishna
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Graphical analysis-based calculation method of concrete strength
AIP AdvancesThis study established a three-dimensional random spherical aggregate model of concrete. Models with various volume contents of coarse aggregate were sliced, and slice areas were statistically analyzed. To study the effects of the coarse aggregate volume
Xiaohui Wang +4 more
doaj +1 more source
Clinical validation of a graphical method for radiation therapy plan quality assessment. [PDF]
Radiat Oncol, 2020 Ventura T +7 more
europepmc +1 more source
Graceful Degradation: a Method for Designing Multiplatform Graphical User Interfaces [PDF]
, 2006 Graceful Degradation: a Method for Designing Multiplatform Graphical User Interfaces(PhD in Management Sciences, option "Information Systems") -- UCL ...
Florins, Murielle
core
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
A study on genotype-environment interaction based on GGE biplot graphical method in sunflower genotypes (Helianthus annuus L.). [PDF]
Food Sci Nutr, 2020 Ansarifard I +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source