Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Ultrasound-Assisted Deep Eutectic Solvent-Based Green Extraction of Flavonoids from Honeysuckle: Optimization and Mechanistic Insights into α-Amylase Inhibition. [PDF]
Zong H +8 more
europepmc +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Upcycling of Citrus Waste by Natural Deep Eutectic Solvents: Green Extraction of Bioactive Compounds with Antioxidant and Regenerative Properties on Human Keratinocytes. [PDF]
Silla A +10 more
europepmc +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Green Extraction of Volatile Terpenes from <i>Artemisia annua</i> L. [PDF]
Mandić M +5 more
europepmc +1 more source
Emerging Trends in Green Extraction Techniques, Chemical Modifications, and Drug Delivery Systems for Resveratrol. [PDF]
Trombino S +3 more
europepmc +1 more source
Exploring alternative solvents to n-hexane for green extraction of lipid from camellia oil cakes. [PDF]
Lin Y, Wang Y, Li Y.
europepmc +1 more source
<i>Bacillus</i> Pectinases as Key Biocatalysts for a Circular Bioeconomy: From Green Extraction to Process Optimization and Industrial Scale-Up. [PDF]
Kaissar FZ +8 more
europepmc +1 more source

